Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,581,266	G→T	A44D (GCC→GAC)	ttgB ←	efflux pump membrane protein TtgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,581,266	0	G	T	100.0%	94.2 / NA	30	A44D (GCC→GAC)	ttgB	efflux pump membrane protein TtgB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/11);  total (19/11)

GCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTTTACTC  >  NC_002947/1581122‑1581405                                                                                                                                                 |                                                                                                                                            gCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACggcgatgtcga                                                                                                                                          >  2:152474/1‑148 (MQ=255)          gTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTggcg                                                                                                                                 >  8:152182/1‑148 (MQ=255)                 tgcCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGAt                                                                                                                          <  7:26957/148‑1 (MQ=255)                     gTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTg                                                                                                                      >  7:133203/1‑148 (MQ=255)                       cGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTggg                                                                                                                    <  3:13969/148‑1 (MQ=255)                                tttCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGt                                                                                                            >  7:150012/1‑147 (MQ=255)                                          cacaTAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATTTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGc                                                                                                 >  7:42851/1‑148 (MQ=255)                                              tAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCa                                                                                             >  8:99040/1‑148 (MQ=255)                                              tAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGACACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCa                                                                                             >  4:36040/1‑148 (MQ=255)                                                 cGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGa                                                                                          >  1:14973/1‑148 (MQ=255)                                                 cGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGa                                                                                          <  2:13155/148‑1 (MQ=255)                                                  gCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGAt                                                                                         >  5:156993/1‑148 (MQ=255)                                                     ggTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGa                                                                                      <  6:156993/148‑1 (MQ=255)                                                           cGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAgg                                                                                 <  5:55805/147‑1 (MQ=255)                                                            gATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGcc                                                                               >  2:28380/1‑148 (MQ=255)                                                            gATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGcc                                                                               >  1:25569/1‑148 (MQ=255)                                                             aTACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGccc                                                                              <  5:162383/148‑1 (MQ=255)                                                                 cGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGAGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGa                                                                           >  8:153738/1‑147 (MQ=255)                                                                  gTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGCTGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGa                                                                           >  7:519/1‑146 (MQ=255)                                                                        ctgctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATg                                                                   >  2:150560/1‑148 (MQ=255)                                                                           ctgctCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATc                                                                >  3:75747/1‑148 (MQ=255)                                                                               tCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCAc                                                              >  3:144658/1‑146 (MQ=255)                                                                                 gATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAg                                                          >  2:101088/1‑148 (MQ=255)                                                                                      ccTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGa                                                      >  6:9603/1‑147 (MQ=255)                                                                                            ccacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCa                                               >  8:4445/1‑148 (MQ=255)                                                                                             cacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAg                                              >  2:133892/1‑148 (MQ=255)                                                                                             cacGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAg                                              <  7:99040/148‑1 (MQ=255)                                                                                                  tgtCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGACACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGaa                                         <  3:36040/148‑1 (MQ=255)                                                                                                          aCGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCgg                                   <  1:152474/146‑1 (MQ=255)                                                                                                                                        aCGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGGACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTTTACt   <  8:133203/148‑1 (MQ=255)                                                                                                                                         cGGCGATGTCGATGGCTGGCGGCGCGATACTGGGGTACTGGGTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTGCGACATGGTTTACTc  <  5:81213/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                            GCGGTAATGGTCATGCTGCCGTCGGAGTTACTTTCCGACGACACATAACGCAGGTTGTCGATACCGTTGAGCTGCTGCTCGATCACCTGCACCACGGTGTCCTGCACGGTTTGCGCCGAGGCGCCCGGGTAGGTCACGGCGATGGCGATGGCTGGCGGCGCGATACTGGGGTACTGGTTGATCGGCAGCTTCAGGATGGACAAGGCCCCGACCAGCATGATCACCAAGGCGATCACCCAGGCGAAGATCGGGCGATCGATAAAGAACTTCGACATGGTTTACTC  >  NC_002947/1581122‑1581405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑