Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,202,220	G→A	intergenic (+77/‑104)	PP_1946 → / → PP_1947	short‑chain dehydrogenase/reductase family oxidoreductase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,202,220	0	G	A	100.0%	100.7 / NA	31	intergenic (+77/‑104)	PP_1946/PP_1947	short‑chain dehydrogenase/reductase family oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

CATTCGATGTCAGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAGTTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGATTTGGTTAAGGTGC  >  NC_002947/2202075‑2202351                                                                                                                                                  |                                                                                                                                    cATTCGATGTCAGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAAtt                                                                                                                                   >  3:71360/1‑148 (MQ=255)   ttCGATGTCAGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAAtttt                                                                                                                                 >  7:89448/1‑148 (MQ=255)      gATGTCAGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTAt                                                                                                                               <  4:79921/147‑1 (MQ=255)            aGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATTGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCt                                                                                                                        <  2:147901/148‑1 (MQ=255)            aGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATc                                                                                                                         >  1:97562/1‑147 (MQ=255)            aGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATc                                                                                                                         >  3:10136/1‑147 (MQ=255)                gACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGaaa                                                                                                                    >  2:167737/1‑148 (MQ=255)                  cGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAAtt                                                                                                                  <  2:84241/148‑1 (MQ=255)                                   cTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGAttttt                                                                                                  <  1:13978/147‑1 (MQ=255)                                       ggTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCt                                                                                              <  1:41191/147‑1 (MQ=255)                                              aCTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGtat                                                                                      <  1:167737/148‑1 (MQ=255)                                                  gAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTACTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACATGTGGGtatttat                                                                                  >  6:140584/1‑148 (MQ=255)                                                   aaTGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGtatttatt                                                                                 <  3:152341/148‑1 (MQ=255)                                                   aaTGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGtattta                                                                                   <  2:49590/146‑1 (MQ=255)                                                      ggATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTGATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCa                                                                              <  6:23459/148‑1 (MQ=255)                                                          gatCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTATCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCt                                                                          >  3:47246/1‑148 (MQ=255)                                                                      aTCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTa                                                              >  8:151747/1‑148 (MQ=255)                                                                            gTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACttttt                                                        <  3:85555/148‑1 (MQ=255)                                                                                   tGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGAGTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACt                                                  >  4:111037/1‑147 (MQ=255)                                                                                               aTGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGga                                      <  4:71360/147‑1 (MQ=255)                                                                                               aTGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGga                                      <  2:153107/147‑1 (MQ=255)                                                                                                       gcCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAAATTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCaa                              <  4:24001/147‑1 (MQ=255)                                                                                                         cTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAAtt                            >  7:93545/1‑147 (MQ=255)                                                                                                                  gCCATTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAAt                     >  7:107074/1‑145 (MQ=255)                                                                                                                     aTTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGAt               <  3:18012/148‑1 (MQ=255)                                                                                                                     aTTGCTTGGTAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGAt               >  7:138205/1‑148 (MQ=255)                                                                                                                      ttGCTTGGTAAGCCTAGGCTCACTATAAATTTTATTTGATCTGAAATTTGGTTTAGTGATTTTTACCTGTGGGTATTTATTGCACGCTGACAGCCGCCTACTTTTTGCCACAGCAATAAATGGAGAGTTCAATTGAAGAATCAAGAtt              >  4:78074/1‑148 (MQ=255)                                                                                                                              tAAGGCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGATTTGGTTAAg      >  2:24683/1‑148 (MQ=255)                                                                                                                               aaGGCTAGGCTCACTATAAAGTATATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTTGGTATTTATTGCACGCTGGCTGCCGCCTACTTTTTGCCACTGAAATAAATGGAGAGTTCAATTGAACAATCACGATTTGGTTAAg      <  8:62199/147‑1 (MQ=255)                                                                                                                                  gCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGATTTGGTTAAGGTGc  >  8:141481/1‑148 (MQ=255)                                                                                                                                  gCTAGGCGCACTATAAATTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGACACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGATTTGGTTAAGGTGc  >  8:131928/1‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                    CATTCGATGTCAGTCGACGGTGGCGTGGCAGTGACTGCGGTTGGTACTCGAATGGATGATCTCTTTTAAATCCATGTAGCGTTGCTGGTTATGAATGGATGCGCCTAGTTCTCGCCATTGCTTGGTAAGGCTAGGCGCACTATAAGTTTTATTTGATCTGAAATTTGGTTTTGTGATTTTTACCTGTGGGTATTTATTGCACGCTGTCTGCCGCCTACTTTTTGCCACTGCAATAAATGGAGAGTTCAATTGAACAATCAAGATTTGGTTAAGGTGC  >  NC_002947/2202075‑2202351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑