Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,722,068	C→T	Q197* (CAG→TAG)	PP_2382 →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,722,068	0	C	T	100.0%	82.0 / NA	25	Q197* (CAG→TAG)	PP_2382	putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/10);  total (15/10)

CGCCGCCTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAACAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTC  >  NC_002947/2721923‑2722184                                                                                                                                                  |                                                                                                                     cgccgcCTCGATCCTCGCGCCGCTGGTCTTTTCGGCCTGTCTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAg                                                                                                                    >  2:66411/1‑148 (MQ=255)  gccgccTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAg                                                                                                                    >  3:73251/1‑147 (MQ=255)    cgccTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCa                                                                                                                 >  6:92447/1‑148 (MQ=255)     gccTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCAt                                                                                                                >  3:69462/1‑148 (MQ=255)     gccTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCAt                                                                                                                >  5:28906/1‑148 (MQ=255)           aTCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGAt                                                                                                           <  8:115273/147‑1 (MQ=255)                           tCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCa                                                                                           <  7:140279/147‑1 (MQ=255)                            cTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAgg                                                                                         <  1:11936/148‑1 (MQ=255)                                        tgtgtTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGcggcgg                                                                               >  3:14786/1‑146 (MQ=255)                                          tgtTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAg                                                                           >  3:145317/1‑148 (MQ=255)                                                       tcGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGt                                                                >  7:116143/1‑146 (MQ=255)                                                          agaAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAgg                                                           <  6:71413/148‑1 (MQ=255)                                                          agaAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAgg                                                           <  4:73251/148‑1 (MQ=255)                                                                 aCTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCa                                                    >  3:112966/1‑148 (MQ=255)                                                                       ttGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATgcgc                                              >  1:76210/1‑148 (MQ=255)                                                                                       gCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCAt                              >  4:149506/1‑148 (MQ=255)                                                                                         cTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGGAGTGTCCAATAGGCATTCGATGGGGGCGCCATAATCATGGCTGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCAtt                             <  7:144019/147‑1 (MQ=255)                                                                                          ttATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTgg                           >  7:15052/1‑148 (MQ=255)                                                                                                        aTTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACActct             >  4:20436/1‑148 (MQ=255)                                                                                                         tttGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCtg            >  5:148360/1‑148 (MQ=255)                                                                                                              aTCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGa       >  2:53768/1‑148 (MQ=255)                                                                                                                cGCTTCCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATCCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCTGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGAt      <  4:69462/147‑1 (MQ=255)                                                                                                                   tACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTc  <  5:92447/148‑1 (MQ=255)                                                                                                                   tACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTc  <  2:92276/148‑1 (MQ=255)                                                                                                                   tACCTTGACGCCGGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTc  <  4:132802/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                     CGCCGCCTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAACAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTC  >  NC_002947/2721923‑2722184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑