| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 75.0% | 20.6 / ‑4.3 | 12 | G3494R (GGG→AGG) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (5/4); minor base C (0/3); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921315‑2921600 | cacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGAAGACAGCATGCATGGCGGCAACGGCAATGACACCCTGCAGCGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACACTGATCagg > 8:25439/1‑148 (MQ=255) acGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGCCGTGGGTACCGACACCCTGATCaggg > 4:57423/1‑148 (MQ=255) cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCg > 2:31027/1‑147 (MQ=255) tGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCg > 4:54223/1‑146 (MQ=255) cTGGCGATGACAGCCGGGAGGGCGGCAACGGCAATGCCACCCTGCCGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCATGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAc < 7:106800/148‑1 (MQ=255) gACAGCCTGGAGGGCGGCAACGTCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGGACCGACACCCTGCTCCGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGtggtgg < 1:94527/148‑1 (MQ=255) ccTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGtgggtggg > 2:85662/1‑148 (MQ=255) cggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACAcc > 8:69835/1‑148 (MQ=255) gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGACCCGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa < 7:13492/148‑1 (MQ=255) cGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt < 3:54223/148‑1 (MQ=255) gccGTGGGTACCGACACCCTGCTCAGGGGGGTCGGCAACTACTTCCTCTATGGCGGGGGAGGTAACGATACGGTGGTGGGGGTGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg < 2:26740/146‑1 (MQ=255) gACGTGGGTACCGACCCCCTGATCCGGGGTGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg < 3:48539/148‑1 (MQ=255) ccTGATCAGGGGGGGCGGCTACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGTGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc < 2:57166/148‑1 (MQ=255) ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat > 4:82257/1‑147 (MQ=255) | CACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921315‑2921600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |