Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,718,040	T→A	M202K (ATG→AAG)	fabB →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,718,040	0	T	A	100.0%	56.3 / NA	19	M202K (ATG→AAG)	fabB	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/7);  total (12/7)

ACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGAACTGGAACACGCCCTGGCCCG  >  NC_002947/4717896‑4718175                                                                                                                                                 |                                                                                                                                        actactCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAgg                                                                                                                                       <  5:109709/147‑1 (MQ=255) actactCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAgg                                                                                                                                       <  5:11912/147‑1 (MQ=255)  ctactCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGgc                                                                                                                                     >  7:72927/1‑148 (MQ=255)      tCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCCGTCGTTCCTGTTCGATGCCAAGGGCGccc                                                                                                                                 >  4:129025/1‑148 (MQ=255)        gATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTg                                                                                                                               >  5:70235/1‑148 (MQ=255)          gctcGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTc                                                                                                                             <  6:24417/147‑1 (MQ=255)                         ccACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACg                                                                                                               >  4:7178/1‑147 (MQ=255)                              tCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAAccc                                                                                                         >  1:6272/1‑148 (MQ=255)                              tCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAAccc                                                                                                         >  5:110653/1‑148 (MQ=255)                                                  cccACTGGATCTGCTCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAGGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAACCACCGGAACTGGCCTCGCGCGcc                                                                                     >  8:49526/3‑148 (MQ=255)                                                                                          ttCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCtggcggtg                                             >  8:91695/1‑148 (MQ=255)                                                                                                  tGGCGGTGAAGCAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGGCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCAtg                                     <  8:134194/148‑1 (MQ=255)                                                                                                     cGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCAtggtc                                  <  8:72927/148‑1 (MQ=255)                                                                                                        tGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCAtggtcgtg                               <  2:6272/148‑1 (MQ=255)                                                                                                                       gAGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGAACt                    >  2:79286/1‑144 (MQ=255)                                                                                                                        aGCCAGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGAACTg                   <  1:79286/144‑1 (MQ=255)                                                                                                                            aGTCGTTCCTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGAACTGGAACACGc           >  7:48790/1‑148 (MQ=255)                                                                                                                                   ccTGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGAACTGGAACACGCCCTGGcc    >  8:153460/1‑148 (MQ=255)                                                                                                                                     tGTTCGATGCCAAGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGACCTGGAACCCGCCCTGGCCcg  >  5:20479/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                        ACTACTCGATCTCGTCGGCCTGCGCCACCTCGGCTCACTGCATCGGTACCGCACTGGAGCAGATCCAGTGGGGCAAGCAGGACATCGTCTTCGCCGGTGGCGGTGAAGAAGAGCACTGGAGCCAGTCGTTCCTGTTCGATGCCATGGGCGCCCTGTCGACCAAGCGCAACGAAACCCCGGAACTGGCCTCGCGCGCCTACGACGCTGACCGTGATGGCTTCGTCATCGCTGGCGGTGGCGGCATGGTCGTGGTCGAGGAACTGGAACACGCCCTGGCCCG  >  NC_002947/4717896‑4718175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑