Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,947,807 C→A V223L (GTG→TTG)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,947,8070CA100.0% 144.7 / NA 43V223L (GTG→TTG) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/19);  total (24/19)

CCAGTCGAGTGATCGAGTCCAGCAGGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCACGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCGGCTTTCGGCGGGGCAACGATCAG  >  NC_002947/5947663‑5947951
                                                                                                                                                |                                                                                                                                                
ccAGTCGAGTGATCGAGTCCAGCAGGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAGCCACTTCGCCACGCAAgga                                                                                                                                               >  2:53912/1‑147 (MQ=255)
      gAGTGATCGAGTCCAGCAGGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCATCCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCACCCACTTCGCCACGCAAGGTGCGCt                                                                                                                                          >  7:57073/1‑147 (MQ=255)
          gATCGAGTCCAGCAGGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCAtt                                                                                                                                     >  7:17173/1‑148 (MQ=255)
            tCGAGTCCAGCAGGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTc                                                                                                                                   >  4:76139/1‑148 (MQ=255)
                    agcagGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCActt                                                                                                                           >  7:34733/1‑148 (MQ=255)
                    agcagGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCActt                                                                                                                           >  6:34294/1‑148 (MQ=255)
                              ccACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTTGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCCACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACGTCTTCCGGgcg                                                                                                                 >  4:24743/1‑148 (MQ=255)
                                 caTCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCtc                                                                                                              <  3:133822/148‑1 (MQ=255)
                                  aTCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTc                                                                                                                                   >  2:516/1‑126 (MQ=255)
                                   tctttcttGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCg                                                                                                                                  <  1:516/126‑1 (MQ=255)
                                            tgCTCGACCAGGCTCTTGGCCTTCTCGATCGCCATCTCGGCACCCTGCACGTGGCGGGTTGGCGGCTCGTCGCAGGTTGAAGCAACCACTTCGCCCCGCAAGGTGCGCTGCAGTTCGGTCACTTCTTCCGGGCGCTCGTCGATcagca                                                                                                   >  6:47381/1‑148 (MQ=255)
                                            tgCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATcagca                                                                                                   >  2:111104/1‑148 (MQ=255)
                                            tgCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATcagc                                                                                                    <  4:45107/147‑1 (MQ=255)
                                            tgCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATcagc                                                                                                    <  3:77773/147‑1 (MQ=255)
                                            tgCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATcagc                                                                                                    <  3:80387/147‑1 (MQ=255)
                                             gCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATcagca                                                                                                   >  8:78071/1‑147 (MQ=255)
                                                         gcTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTg                                                                                      >  7:97352/1‑148 (MQ=255)
                                                           ttGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGc                                                                                    >  7:155368/1‑148 (MQ=255)
                                                             ggCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCg                                                                                                                     >  4:30367/1‑113 (MQ=255)
                                                              gCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCgg                                                                                                                    <  3:30367/113‑1 (MQ=255)
                                                                cTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCa                                                                               <  5:57882/148‑1 (MQ=255)
                                                                 ttCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAg                                                                              >  8:79545/1‑148 (MQ=255)
                                                                   ctcGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAggg                                                                            >  6:74704/1‑148 (MQ=255)
                                                                    tcGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAggg                                                                            <  6:147465/147‑1 (MQ=255)
                                                                        tCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGggtgtt                                                                       >  8:116389/1‑148 (MQ=255)
                                                                             aTCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACggg                                                                  >  8:153782/1‑148 (MQ=255)
                                                                                   gCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGa                                                               <  8:72574/145‑1 (MQ=255)
                                                                                     aaCCTGCACGTTGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCTCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTg                                                          <  3:87004/148‑1 (MQ=255)
                                                                                        cTGCACGTGGCGGGTTGGTGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGcc                                                       >  8:138944/1‑148 (MQ=255)
                                                                                                ggCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGtt                                               <  8:17173/148‑1 (MQ=255)
                                                                                                ggCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACCTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGtt                                               <  4:148177/148‑1 (MQ=255)
                                                                                                   gggTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTg                                            <  1:111104/148‑1 (MQ=255)
                                                                                                    ggTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCt                                             >  3:53097/1‑146 (MQ=255)
                                                                                                              tcgtcgAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCg                                 <  8:97352/148‑1 (MQ=255)
                                                                                                                 tcgAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGt                                <  1:164628/146‑1 (MQ=255)
                                                                                                                 tcgAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTc                               >  1:86458/1‑147 (MQ=255)
                                                                                                                  cgAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCtt                             <  5:140898/148‑1 (MQ=255)
                                                                                                                   gAAGGTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTa                            >  3:81740/1‑148 (MQ=255)
                                                                                                                       gTGGAAGCAACCACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCgg                        <  2:86458/148‑1 (MQ=255)
                                                                                                                            aGCCACCACTGCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCGCGTCGATCAGCAGGACGATCAGGGGGCACGCACGGTTGTTATGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCGGCTTTc                   <  6:123668/148‑1 (MQ=255)
                                                                                                                                 ccACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCGGCTTTcggcgg              <  1:14183/148‑1 (MQ=255)
                                                                                                                                  cACTTCGCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCGGCTTTCGGCggg             >  1:113212/1‑148 (MQ=255)
                                                                                                                                        gCCACGCAAGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCGGCTTTCGGCGGGGCAACg       >  3:70539/1‑148 (MQ=255)
                                                                                                                                             gcaagGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGAGCAGGTGGCACTCAGGGTTGTGACGGGTGATGTTGGCCGCAATGGTCTGCAGCATGATCGTCTTACCGGCTTTCGGCGGGGCAACGATCAg  <  3:24931/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CCAGTCGAGTGATCGAGTCCAGCAGGATAACCACATCTTTCTTGTGCTCGACCAGGCGCTTGGCCTTCTCGATCACCATCTCGGCAACCTGCACGTGGCGGGTTGGCGGCTCGTCGAAGGTGGAAGCAACCACTTCGCCACGCACGGTGCGCTGCATTTCGGTCACTTCTTCCGGGCGCTCGTCGATCAGCAGGACGATCAGGTGGCACTCAGGGTTGTTACGGGTGATGTTGGCCGCAATGTTCTGCAGCATGATCGTCTTACCGGCTTTCGGCGGGGCAACGATCAG  >  NC_002947/5947663‑5947951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑