Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,212,521	C→T	intergenic (‑55/+133)	PP_1060 ← / ← PP_1061	glutamate synthase large subunit/ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,212,521	0	C	T	100.0%	109.2 / NA	34	intergenic (‑55/+133)	PP_1060/PP_1061	glutamate synthase large subunit/ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/11);  total (23/11)

CCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATT  >  NC_002947/1212377‑1212663                                                                                                                                                 |                                                                                                                                               ccATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACtata                                                                                                                                             <  5:105850/148‑1 (MQ=255)   aTTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAg                                                                                                                                            >  7:141778/1‑147 (MQ=255)   aTTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGCGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAg                                                                                                                                            >  3:148056/1‑147 (MQ=255)                    gggAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACt                                                                                                                           >  2:17892/1‑147 (MQ=255)                      gAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACACCGACTATAGCCAAGATGCCGGGCACTGc                                                                                                                         >  4:114950/1‑147 (MQ=255)                             gCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCaaaataaa                                                                                                                 >  1:61471/1‑148 (MQ=255)                               cAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCaaaataaaat                                                                                                               >  2:150605/1‑148 (MQ=255)                                 gggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGGCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCg                                                                                                             <  1:17662/148‑1 (MQ=255)                                  ggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCgg                                                                                                            <  4:93834/148‑1 (MQ=255)                                  ggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCgg                                                                                                            <  5:138939/148‑1 (MQ=255)                                   gTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCggg                                                                                                           <  2:180859/148‑1 (MQ=255)                                       aCAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAg                                                                                                       >  5:53057/1‑148 (MQ=255)                                           cacacaGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGa                                                                                                    >  3:45932/1‑147 (MQ=255)                                             cacaGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGaaaa                                                                                                 >  6:86044/1‑148 (MQ=255)                                                  gcggcgAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCAtt                                                                                            <  6:53057/148‑1 (MQ=255)                                                     gcgAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGTGTCCTTGCGTTGGCGCTGTCAGGCTCAGGGCCTGGGGGAAAACCCCGACTATAGCCAAGTTGCCGTGCACTGCAAAATAATCTCGGGGTAGCGAAAATCATtctc                                                                                         <  8:80247/148‑1 (MQ=255)                                                          gaagGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGc                                                                                     >  7:94725/1‑147 (MQ=255)                                                          gaagGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGc                                                                                     <  8:141778/147‑1 (MQ=255)                                                               cATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGa                                                                               >  7:47843/1‑148 (MQ=255)                                                                 tAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGc                                                                             >  8:13922/1‑148 (MQ=255)                                                                     gACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGt                                                                         >  8:89301/1‑148 (MQ=255)                                                                                  cAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGaaa                                                             <  6:97359/147‑1 (MQ=255)                                                                                             aGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCctg                                                 >  1:22397/1‑148 (MQ=255)                                                                                                 tCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTgg                                             <  4:82098/148‑1 (MQ=255)                                                                                                    ttGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCa                                          >  1:4022/1‑148 (MQ=255)                                                                                                            gcgcGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCAc                                  >  2:11141/1‑148 (MQ=255)                                                                                                                    aGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTg                          >  5:36042/1‑148 (MQ=255)                                                                                                                        tCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGAGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCCCCCGCCGTGCAcc                      >  2:3178/1‑148 (MQ=255)                                                                                                                          aGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGt                    >  2:172261/1‑148 (MQ=255)                                                                                                                                     gaaaaCCCCTACTATAGCCTAGTTGCCGTGCACTGCAAAATAAATTCGGGGTAGCGTAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTGCACCCGCCGTGCACCGTTGCCGCCGCTa         <  4:148056/146‑1 (MQ=21)                                                                                                                                     gCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGTTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTa         >  6:150279/1‑148 (MQ=255)                                                                                                                                     gCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTa         <  1:150605/148‑1 (MQ=255)                                                                                                                                           cccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCGCCCGCCGTGCACCGTTGCCGCCGCTACCGTAt   >  1:82219/1‑148 (MQ=255)                                                                                                                                            ccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTAtt  >  1:104379/1‑148 (MQ=255)                                                                                                                                            ccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTAtt  >  8:12087/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                               CCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATT  >  NC_002947/1212377‑1212663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑