Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	100.0 / NA	33	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/17);  total (16/17)

AAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGG  >  NC_002947/1499365‑1499647                                                                                                                                                |                                                                                                                                               aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTGCCGCGCAACAGGCGCCGCGGGGGATGGCCCCGGCTTTGGCGGTGTTAGCGGGCAcg‑cccgcg                                                                                                                                          >  5:310360/1‑145 (MQ=255)    cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTcc                                                                                                                                         >  5:286123/1‑148 (MQ=25)       gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGCATGGCACCGGCGTTTCCGGTGTTAGCGGGCACGCCCGCTCCccca                                                                                                                                     >  8:202334/1‑149 (MQ=25)                       tagCCTCACCGAAGCCTTGGCACCACAGCAGTAGTGTGGCTGCCAAAGGGGGGGAGCGGGCGGGCCGGCTAAACTGGCGCCGCGGTGATTGGCACGGGCTTTGCGGTTGTTCGCGGGCAGGCCCGCTCCCACAGACTGATT‑GCCATTaa                                                                                                                    <  8:219231/148‑1 (MQ=255)                         gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGc                                                                                                                  <  1:72325/149‑1 (MQ=21)                          ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCCCGCCCGCGCCCCCAGACTGATT‑GCCATTAAGCg                                                                                                                 >  6:296498/1‑149 (MQ=21)                           cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCACGCCCGCGACCACAGACTGCTT‑GCCATTAAGCgg                                                                                                                >  8:374819/1‑149 (MQ=21)                                 gaagaattGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCGTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTAt                                                                                                          >  5:279494/7‑149 (MQ=21)                                         ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACa                                                                                                   <  6:286123/148‑1 (MQ=21)                                              cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTAGCCATTAAGCGGGCCTATCGCTACAGTGAc                                                                                              <  1:239111/149‑1 (MQ=21)                                                           aGGCTGCCAATCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                                  <  5:296498/147‑1 (MQ=21)                                                           aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                                  <  3:273347/147‑1 (MQ=21)                                                           aGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                                  <  4:305993/147‑1 (MQ=21)                                                                   aaaCGGGGGGCGCGGGCGGGCCCGCGAAACCGGCGCCGCGGGCCAGGGCCCCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc                                                                        <  7:166965/149‑1 (MQ=25)                                                                              gcgggcggCCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTCCCGGTGTTCGCGGCCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                             <  5:33343/149‑1 (MQ=25)                                                                              gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt                                                             <  7:202334/149‑1 (MQ=25)                                                                                gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa                                                           <  6:298114/149‑1 (MQ=37)                                                                                        ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt                                                     >  4:311801/1‑147 (MQ=37)                                                                                        ccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa                                                   <  3:311801/149‑1 (MQ=37)                                                                                          gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg                                                  >  1:164629/1‑148 (MQ=37)                                                                                            gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                                                >  2:155892/1‑148 (MQ=37)                                                                                            gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa                                                <  1:155892/148‑1 (MQ=37)                                                                                            gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc                                               >  6:215383/1‑149 (MQ=37)                                                                                            gAAACAGGCGACGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGTCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc                                               <  6:310360/149‑1 (MQ=37)                                                                                              aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc                                             >  2:87917/1‑149 (MQ=37)                                                                                                 aGGCGCCGCGGTGCATGGCACCGTCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt                                          <  3:259509/149‑1 (MQ=37)                                                                                                   gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                         >  8:189695/1‑148 (MQ=37)                                                                                                   gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                         >  1:332148/1‑148 (MQ=37)                                                                                                   gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc                                         >  8:284041/1‑148 (MQ=37)                                                                                                           gTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc                                <  7:374819/149‑1 (MQ=38)                                                                                                                              ccGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt             >  6:277804/1‑149 (MQ=255)                                                                                                                              ccGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActtctt             >  8:5140/1‑149 (MQ=255)                                                                                                                                       gcGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgc    >  5:329380/2‑149 (MQ=255)                                                                                                                                         gggCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcgg  <  8:313475/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               AAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGG  >  NC_002947/1499365‑1499647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑