Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	100.0%	122.9 / NA	38	D70V (GAT→GTT)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (20/18);  total (20/18)

ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335980‑336263                                                                                                                                                  |                                                                                                                                             aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttac                                                                                                                                          <  5:358555/149‑4 (MQ=255)       cccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                     >  3:286005/1‑149 (MQ=255)        ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                     <  4:313950/148‑1 (MQ=255)          tCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGa                                                                                                                                   >  2:127322/1‑148 (MQ=255)             aTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATg                                                                                                                               <  5:92090/149‑1 (MQ=255)                  cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAACCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCaa                                                                                                                          <  1:127322/149‑1 (MQ=255)                       cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGCCCATTAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgg                                                                                                                     >  5:117608/1‑148 (MQ=255)                       cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACGTGGCCAAGgcgc                                                                                                                     <  4:108931/149‑1 (MQ=255)                       cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGAGATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                     >  7:204861/1‑149 (MQ=255)                        gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGCGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCt                                                                                                                    <  8:186512/149‑1 (MQ=255)                        gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                     <  4:339977/132‑1 (MQ=255)                        gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGc                                                                                                                                     >  3:339977/1‑132 (MQ=255)                           gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCtgat                                                                                                                 <  3:229418/149‑1 (MQ=255)                                    gTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                        <  2:309466/116‑6 (MQ=255)                                    gTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑ttacct                                                                                                                                        >  1:309466/1‑111 (MQ=255)                                       cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                       >  3:99376/1‑147 (MQ=255)                                                                    gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑t                                                                       >  8:196607/1‑149 (MQ=255)                                                                               gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAACCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCa                                                            >  2:337040/1‑149 (MQ=255)                                                                                 tACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCAtt                                                          >  3:375493/1‑149 (MQ=255)                                                                                   cATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTACTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTtg                                                        >  3:220646/1‑149 (MQ=255)                                                                                      cAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGACAAGGGTAAGGCGCATTAGGCA‑TTGACGAGGTCATTTGTgg                                                     >  7:363346/1‑149 (MQ=255)                                                                                        aGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGa                                                   <  4:375493/149‑1 (MQ=255)                                                                                           aCCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCg                                                >  5:24443/1‑149 (MQ=255)                                                                                                gTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCtt                                           <  8:302465/149‑1 (MQ=255)                                                                                                gTGCGCGAGGGCGATATGGAGGACGACCATAAATTACTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCtt                                           >  7:302465/1‑149 (MQ=255)                                                                                                  gcgcGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         >  1:71791/1‑149 (MQ=255)                                                                                                    gcgATGGCGTTATGGAGGACGACCATAATTTCCTGGTGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATTTCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt                                       <  6:24443/149‑1 (MQ=255)                                                                                                      gAGGGCGATAGGGAGGAAGACCATAAATTCCTGCGGTCAGCCGATTACCTGGGCGACATGGGCAAGGCGCTGATGAACGATATCGAGATCGGCAAGGGTAAGGGGCATTAGGCA‑TTGACGAGGTCATTTGTGGGAGCGGCGTTGCGTcg                                     <  2:127474/149‑1 (MQ=255)                                                                                                       aGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcgc                                    <  8:204861/149‑1 (MQ=255)                                                                                                              tatGGAGGACGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATggg                             >  2:310772/1‑149 (MQ=255)                                                                                                                tGGAGGACGACCATAAATTACTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCt                           <  4:220646/149‑1 (MQ=255)                                                                                                                   aggaCGACCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGAGTCGCGAATGGGCTGc                         >  1:106212/1‑148 (MQ=255)                                                                                                                     gacgacCATAAATTCCTGGGGGCAGCCGATTACCTGTGCGAGATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCaaa                      <  5:343848/149‑1 (MQ=255)                                                                                                                     gacgacCATAAATTCCTGGGGGCAACCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCaaa                      <  1:337040/149‑1 (MQ=255)                                                                                                                           cATAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcagc                 <  1:310772/149‑1 (MQ=255)                                                                                                                             tAAATTCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGcccc              >  5:209246/1‑149 (MQ=255)                                                                                                                                  tCCTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGa         >  8:10255/1‑149 (MQ=255)                                                                                                                                    cTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAtt       >  7:209725/1‑149 (MQ=255)                                                                                                                                    cTGGGGGCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAtt       >  4:345235/1‑149 (MQ=255)                                                                                                                                       gcggCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGATATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCa    <  7:371409/147‑1 (MQ=255)                                                                                                                                         ggCAGCCGATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  4:286005/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                             ACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCA‑TTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335980‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑