Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,419,549	+C	intergenic (‑38/+98)	PP_1242 ← / ← PP_1244	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,419,548	1	.	C	100.0%	136.1 / NA	41	intergenic (‑37/+99)	PP_1242/PP_1244	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (16/25);  total (16/25)

CCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTA  >  NC_002947/1419400‑1419675                                                                                                                                                       |                                                                                                                                ccTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg                                                                                                                                  >  7:258288/1‑149 (MQ=255) ccTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg                                                                                                                                  >  4:203018/1‑149 (MQ=255)  cTGTCAGGTTATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTGTTCGGCTTGGTAGAAGAAGGTCTTCCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTTGGCTTT‑GTTTTGTTTCCCTGTATTCTGGGTTGCCTGggctgg‑c                                                                                                                                <  6:23033/149‑1 (MQ=255)    ggCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑cc                                                                                                                               >  3:76326/1‑147 (MQ=255)          ggATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTcgc                                                                                                                         <  2:28799/149‑1 (MQ=255)             tGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCggg                                                                                                                      <  4:329752/149‑1 (MQ=255)                       gATGTGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCt                                                                                                            >  3:381963/1‑149 (MQ=255)                       gATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGc                                                                                                             <  7:381197/148‑1 (MQ=255)                       gATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCt                                                                                                            >  8:166876/1‑149 (MQ=255)                        ctGCGGAACCGGGGGTGTGTGTTGTTTTCGCCTTGGTAGATGCAGGTCTTCCCGACTGTGGTGGGTTCTGTTGTGTTGTAGGTGGGCATT‑GTTTTTTTTCCCTGGATTCTGGGTTGCCTGGTCTGGCCTCTTCGCGTGCACGCCCGCTc                                                                                                           <  8:258288/148‑1 (MQ=255)                          gCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGcccc                                                                                                          >  7:4504/1‑148 (MQ=255)                            ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                <  3:104283/123‑1 (MQ=255)                            ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                >  4:104283/1‑123 (MQ=255)                              aaCAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAg                                                                                                     <  2:120933/149‑1 (MQ=255)                               aCAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAgg                                                                                                    <  4:159147/149‑1 (MQ=255)                                   cGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                <  5:258419/149‑1 (MQ=255)                                   cGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                <  1:308607/149‑1 (MQ=255)                                             ttgttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCg                                                                                      >  7:216108/1‑149 (MQ=255)                                               gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACACGTACTGTTCAGTGGGt                                                                                     >  5:326182/1‑148 (MQ=39)                                                ttttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                                   >  5:304036/1‑149 (MQ=255)                                                ttttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                                   >  7:241761/1‑149 (MQ=255)                                                 tttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTTTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAc                                                                                  >  8:311560/1‑149 (MQ=255)                                                            tAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGa                                                                       <  8:2705/149‑1 (MQ=255)                                                              gAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAc                                                                      <  3:203018/148‑1 (MQ=255)                                                                 gCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTAGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCt                                                                   >  1:94767/1‑149 (MQ=255)                                                                    ggTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCCCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTgg                                                               <  8:4504/149‑1 (MQ=255)                                                                      tCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTgg                                                               >  8:342211/1‑147 (MQ=255)                                                                      tCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGa                                                             <  7:342211/149‑1 (MQ=255)                                                                          gCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACgg                                                                                >  1:43318/1‑126 (MQ=255)                                                                          gCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACgg                                                                                <  2:43318/126‑1 (MQ=255)                                                                           ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg                                                        >  5:43209/1‑149 (MQ=255)                                                                            cGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGTAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                       <  2:387568/149‑1 (MQ=255)                                                                            cGACTGTGGTGGGTTCTGTTGTGTTGGCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                       <  2:132709/149‑1 (MQ=255)                                                                              aCTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTTTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGt                                                     <  7:311560/149‑1 (MQ=255)                                                                              aCTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGt                                                     <  6:43209/149‑1 (MQ=255)                                                                                    gtggGTTCTGTTGTGTTGTCGGTGGGCGTT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcg                                                <  6:378429/148‑1 (MQ=255)                                                                                    gtggGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcgc                                               <  8:241761/149‑1 (MQ=255)                                                                                    gtggGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcgc                                               <  8:259994/149‑1 (MQ=255)                                                                                     tggGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCcgcg                                              <  2:94767/149‑1 (MQ=255)                                                                                            tgttgttttgTCGGTGGTCTTT‑GTTTTGTTTCCGTGGTTTCGGGGTTGCCTGGGCTGGCCTCTTCGGGGGCACGCCCGCTCCCACAGGTAGTGTGCAGGGCGTGACGGGTGTGGTGACCCTGTGGGTGGGGGCGTGCCCGCGAAGAGGc                                       <  6:304036/149‑1 (MQ=25)                                                                                              ttgtgttgtCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAaga                                          >  7:125753/1‑144 (MQ=255)                                                                                              ttgtgttgtCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAaga                                          <  8:125753/144‑1 (MQ=255)                                                                                                          tGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGGGTGACGGGTGTGGTGACCGTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATg                         >  7:181924/1‑149 (MQ=37)                                                                                                                           tCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                       >  5:375030/1‑103 (MQ=255)                                                                                                                           tCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                       <  6:375030/103‑1 (MQ=255)                                                                                                                                 ttgtgtGGGTTCCCGGGGCGGCCCTCTGCGCGGCCCCGCCGGCCCCCACTGGTACTGGGCTGGGGGGGCGGGCGGGGGTGACCTTGTGGGCGGGGGGGGGCCCGCTAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTggg    <  4:381963/143‑1 (MQ=255)                                                                                                                                   ttCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTa  >  1:59011/1‑148 (MQ=255)                                                                                                                                                       |                                                                                                                                CCTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATT‑GTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTA  >  NC_002947/1419400‑1419675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑