| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 91.7% | 93.1 / ‑5.3 | 36 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base A (14/19); total (16/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 100.0% | 105.5 / NA | 36 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/20); total (16/20) | |||||||||||
GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGG‑CTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATC > NC_002947/1499344‑1499617 || ggCCGCAGTTCGACAACGGTGAATCCCGTGATGCGGCCTTTCAGAGACTGAACGAAGCCTTGGCACCACTGCAGGACTGAGGCTGCCATACGGTGGGAGCGGTCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCg < 6:52520/149‑1 (MQ=38) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCgg > 7:219231/1‑149 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCgg < 4:252813/149‑1 (MQ=255) gCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGGCCGCGGACCAGGCGCCGCCGGGCATGGCCCCGGCCTTTGCCg > 8:166965/1‑149 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGttcgc < 2:118956/149‑4 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGttcgcgg < 7:375805/148‑6 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTTGCCGCGCAACAGGCGCCGCGGGGGATGGCCCCGG‑CTTTGGCGGTGTTAGCGGGCAcg‑cccgcg > 5:310360/1‑145 (MQ=255) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTcc > 5:286123/1‑148 (MQ=25) gTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGGGCATGGCACCGG‑CGTTTCCGGTGTTAGCGGGCACGCCCGCTCCccca > 8:202334/1‑149 (MQ=25) tagCCTCACCGAAGCCTTGGCACCACAGCAGTAGTGTGGCTGCCAAAGGGGGGGAGCGGGCGGGCCGGCTAAACTGGCGCCGCGGTGATTGGCACGGG‑CTTTGCGGTTGTTCGCGGGCAGGCCCGCTCCCACAGACTGATT‑GCCATTaa < 8:219231/148‑1 (MQ=255) gCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGc < 1:72325/149‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCCCGCCCGCGCCCCCAGACTGATT‑GCCATTAAGCg > 6:296498/1‑149 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTAGCGGGCACGCCCGCGACCACAGACTGCTT‑GCCATTAAGCgg > 8:374819/1‑149 (MQ=21) gaagaattGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CGTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTAt > 5:279494/7‑149 (MQ=21) ggCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACa < 6:286123/148‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATTAGCCATTAAGCGGGCCTATCGCTACAGTGAc < 1:239111/149‑1 (MQ=21) aGGCTGCCAATCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 5:296498/147‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 3:273347/147‑1 (MQ=21) aGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 4:305993/147‑1 (MQ=21) aaaCGGGGGGCGCGGGCGGGCCCGCGAAACCGGCGCCGCGGGCCAGGGCCCCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 7:166965/149‑1 (MQ=25) gcgggcggCCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTCCCGGTGTTCGCGGCCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 5:33343/149‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 7:202334/149‑1 (MQ=25) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa < 6:298114/149‑1 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt > 4:311801/1‑147 (MQ=37) ccGCGAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 3:311801/149‑1 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAg > 1:164629/1‑148 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa < 1:155892/148‑1 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGaa > 2:155892/1‑148 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc > 6:215383/1‑149 (MQ=37) gAAACAGGCGACGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGTCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc < 6:310360/149‑1 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 2:87917/1‑149 (MQ=37) aGGCGCCGCGGTGCATGGCACCGT‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt < 3:259509/149‑1 (MQ=37) gcgcCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 8:189695/1‑148 (MQ=37) gcgcCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 1:332148/1‑148 (MQ=37) gcgcCGCGGTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 8:284041/1‑148 (MQ=37) gTGCATGGCACCGG‑CTTTGCCGGTGTTCGCGGGCACGCCCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc < 7:374819/149‑1 (MQ=38) || GGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGG‑CTTTGCCGGTGTT‑TCGGGCACG‑CCGCTCCCACAGACTGATT‑GCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATC > NC_002947/1499344‑1499617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |