Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,932,640	+C	intergenic (‑107/+43)	rluA ← / ← minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,932,638	1	.	C	94.3%	115.7 / 0.4	35	intergenic (‑105/+45)	rluA/minE	bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (17/16);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT  >  NC_002947/1932494‑1932779                                                                                                                                                   |                                                                                                                                               cggcgTCTTCGACGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑c                                                                                                                                               <  2:293342/146‑1 (MQ=255) cggcgTCTTCGAAGAGGAGCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAAAAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑c‑c                                                                                                                                             <  2:282137/147‑1 (MQ=255)   gcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑c‑ccg                                                                                                                                           <  5:314724/147‑3 (MQ=255)               agGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCa                                                                                                                                <  4:256090/147‑1 (MQ=255)                                 aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGt                                                                                                               <  8:123062/146‑1 (MQ=255)                                  gCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTTCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTCCCCAGTAGGAACGGGCTTTG‑C‑CGAGAATGGCTGCAAGGCAGCCGCAAATAGTCg                                                                                                             >  7:204583/1‑147 (MQ=38)                                    ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa                                                                                                            >  7:248084/1‑146 (MQ=255)                                      cATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTa                                                                                                         >  7:309631/1‑147 (MQ=255)                                           cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgcg                                                                                                     <  8:248084/146‑1 (MQ=255)                                            aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGGGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa                                                                                                   <  3:243608/147‑1 (MQ=255)                                               tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa                                                                                                <  6:171996/147‑1 (MQ=255)                                               tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGATCa                                                                                                >  6:285763/1‑147 (MQ=255)                                                ctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATAAGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAg                                                                                               >  8:175941/1‑147 (MQ=255)                                                    cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCg                                                                                             <  2:223251/145‑1 (MQ=255)                                                     gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGGCCCCTGTAGGCGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc                                                                                          <  5:127801/147‑1 (MQ=39)                                                                   ccgggggggCCGCGTGGGGGCCCTTGGGGGCCACCCCCGCTCAAACAAAGTATAGGCGCCCCGTGTTGGCGGGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt                                                                            <  8:309631/142‑1 (MQ=255)                                                                   aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑CGCGCGAAGGGCTGCAAGGCAGCCCCAACTTGTCGACTACGCGGCTCAGCGATCCGGCAGGGTGATGt                                                                            >  5:273150/1‑147 (MQ=255)                                                                      cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa                                                                         >  6:175594/1‑147 (MQ=255)                                                                          ggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGAAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc                                                                     <  2:223206/147‑1 (MQ=255)                                                                           gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCGAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCt                                                                      >  7:207522/1‑145 (MQ=255)                                                                              gCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGa                                                                 <  2:226616/147‑1 (MQ=255)                                                                                tttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAAt                                                               <  8:202012/147‑1 (MQ=255)                                                                                                    aGCCCGCTCCTACAAAGGATATGCGGCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt                                           <  8:191394/147‑1 (MQ=255)                                                                                                       ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa                                        >  2:181251/1‑147 (MQ=255)                                                                                                       ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGAAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCa                                        >  6:179380/1‑147 (MQ=255)                                                                                                              tACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa                                  <  7:175941/146‑1 (MQ=255)                                                                                                               aCAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata                                >  8:248348/1‑147 (MQ=255)                                                                                                                 acaGGAAATGCGTCCCCTGTCGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTTATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata                                <  5:359099/143‑1 (MQ=39)                                                                                                                           cGTCCCCTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg                    >  6:214191/1‑147 (MQ=255)                                                                                                                              ccccTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg                 >  2:95609/1‑147 (MQ=255)                                                                                                                               cccTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc                <  5:175594/147‑1 (MQ=255)                                                                                                                                 cTGTAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg              >  7:116506/1‑147 (MQ=255)                                                                                                                                    tAGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg           >  2:363910/1‑147 (MQ=255)                                                                                                                                     aGGAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt          >  5:31967/1‑147 (MQ=255)                                                                                                                                       gAGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc        >  6:103939/1‑147 (MQ=255)                                                                                                                                        aGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGTATCGAGCAGCTGCCCTTGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca       >  4:298536/1‑147 (MQ=255)                                                                                                                                        aGCGGGC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca       <  5:103939/147‑1 (MQ=255)                                                                                                                                             gC‑TTGCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat  >  2:356656/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                               CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TTG‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT  >  NC_002947/1932494‑1932779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑