Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,126,647	+C	intergenic (‑101/+48)	tdcG‑II ← / ← gcvP‑I	L‑serine dehydratase/glycine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,126,645	1	.	C	88.5%	159.1 / 15.2	52	intergenic (‑99/+50)	tdcG‑II/gcvP‑I	L‑serine dehydratase/glycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (6/0);  new base C (19/27);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 8.70e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAA‑CCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGG  >  NC_002947/1126499‑1126781                                                                                                                                                            |                                                                                                                                             ggAGGGGCTGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCGCGAAT‑GCGGTGGTATAGCCACCATAG‑CATTCG‑C‑GGG‑T‑aaa                                                                                                                                               >  5:374886/1‑147 (MQ=255)  gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAA‑c                                                                                                                                             >  7:281361/1‑147 (MQ=255)      ggtcgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAA‑cccgc                                                                                                                                         <  1:331795/144‑4 (MQ=255)       gccgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAA‑cccgct                                                                                                                                        <  2:263912/147‑5 (MQ=255)           atgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTa                                                                                                                                     <  8:281361/147‑1 (MQ=255)            tgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATAGCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTa                                                                                                                                     <  1:278391/147‑1 (MQ=255)             gccgatCTTCAACCGCTCGAAGACGCTCAGTGACATGGCGCCCTCCTCCCAGGTTGTTGGGTGCACATTGACCCCTCTAGGCGC‑GGG‑ATTACACGCGAAT‑GCGGAGCTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTaca                                                                                                                                   <  1:299939/147‑1 (MQ=25)                 atCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTACCGCGAAT‑TCGGAGGTTTAGGCACCATAG‑CCTTAG‑C‑GGG‑G‑AAACCCGCGCCTACCCag‑a                                                                                                                              >  7:74106/1‑147 (MQ=255)                     tGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTACCGCCCAA‑TCGGAGGGATAGGCACCAACG‑CAATAG‑C‑CGG‑TAAAA‑CCCCTCCCACACAA‑AG‑ccc                                                                                                                         >  4:356202/1‑147 (MQ=255)                       aaCAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTACCCCCAAT‑GCGGAGGGATATCCCCCAACG‑CCTTCG‑CGGGG‑T‑AAC‑CCCCGCCTACACCG‑AG‑ccccc                                                                                                                       >  2:122289/1‑145 (MQ=255)                       aaCAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACag‑a                                                                                                                              <  5:282555/141‑1 (MQ=255)                             tCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCa                                                                                                                 <  6:351750/147‑1 (MQ=255)                                 aGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTAGCC‑GGG‑A‑AAA‑CCGCTCCGAAAAAG‑AG‑ACCGCCACCCaac                                                                                                               >  7:81794/1‑144 (MQ=38)                                  gACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTCCCCCGAAT‑TCGGAGGTATAGCCACCAACG‑CATTAG‑C‑GGG‑T‑AAACCCGCGCCGACACAG‑AG‑CCCGCCACCCCA‑GCC‑a                                                                                                          >  2:264492/1‑147 (MQ=255)                                          gTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGc                                                                                                    <  1:82794/145‑1 (MQ=255)                                                        tcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGG‑TTTACCCCCAAAG‑GGGGGGGT‑TAGCCACCCACGCCATAGC‑C‑GGG‑T‑AAA‑CCCCACCGAAACAA‑AGCCCCCCCCCCCAA‑CCC‑AGG‑GGGGGGGGGGGCGGTCAgg                                                                                    >  2:322616/1‑147 (MQ=255)                                                        tcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTTAACCCCAAAT‑‑AGGGGGTATATCCACCAAAG‑CAATAT‑CGGGG‑T‑AAA‑CCCCGACAAAAAAC‑AA‑CCCCCCCCCCAA‑ACC‑ACGTGGGGGGGGGGGCTaaaaag                                                                                    >  8:107770/1‑142 (MQ=255)                                                            gCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCCCGGAT‑TGGGCGGGATAGGCAACATAG‑CATTAG‑C‑GGGTT‑AAC‑CCGCGCCTACACAC‑AG‑ACCGCCCCCCAA‑ACC‑CGGGGGCGGGGAGGGGTATCAGGCGTc                                                                                >  2:32598/1‑147 (MQ=255)                                                              cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTATCCGCGAAT‑GCGTAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                <  4:259434/145‑1 (MQ=255)                                                              cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCTCCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                <  1:322616/145‑1 (MQ=255)                                                              cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                <  7:107770/145‑1 (MQ=255)                                                              cAGGTTGTGGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGT‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTcc                                                                               <  4:129632/146‑1 (MQ=255)                                                                 tttgttgGGTGGACATGGACCCCTGTCGGGGC‑GGT‑TTTCCGCGCTATG‑GGGGTGGAATAGCCACCATCG‑CATTCG‑G‑GGT‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt                                                                           <  1:345433/146‑1 (MQ=255)                                                                          tGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTAG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcga                                                                  >  2:54864/1‑147 (MQ=255)                                                                                              cgc‑gGG‑TTTCCCCGCGAAA‑GCCGACGTATAGCCACCACCG‑CCCTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAC‑AG‑CCCGCCACCCAA‑GCC‑AGGCGGCGGGGAGGGCTATCAGGCGTCCTGAAAGCTCTCGATCGACGGGCAGGCGCACAcc                                              <  6:207298/147‑1 (MQ=255)                                                                                               gc‑gGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCa                                             >  8:215163/1‑147 (MQ=255)                                                                                                   ggttttACCCGGTATT‑GCGTAGGTATCGCCACAATCG‑CTTTCG‑C‑GGT‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAgg                                           <  5:222699/144‑1 (MQ=255)                                                                                                   gg‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGt                                          <  3:101085/147‑1 (MQ=255)                                                                                                      tttACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAAGGGCAGGCGCACACCAGGTTg                                        >  4:142369/1‑147 (MQ=255)                                                                                                       ttACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc                                       <  1:264492/147‑1 (MQ=255)                                                                                                         aCCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg                                      <  2:50419/146‑1 (MQ=255)                                                                                                           ccGCGAAT‑GCGGAGGTATAGCCACCATCG‑CTTTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt                                    <  4:91828/146‑1 (MQ=255)                                                                                                           ccGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc                                   >  3:43088/1‑147 (MQ=255)                                                                                                           ccGCGAAA‑GCGGAGGTATACCCCCCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCCCCACCCAA‑GCC‑AGGTGGCGGGGAGGGTTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc                                   <  1:103948/147‑1 (MQ=255)                                                                                                            cggtaaT‑GCGGTGGAATACCCACAATCC‑CATTCG‑G‑GGT‑T‑AAACCCGCCCCAACAAAGCCG‑CCCGCCACCCAACGCCTAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCgg                                     <  1:122289/143‑1 (MQ=255)                                                                                                            cgcgAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg                                  >  5:300698/1‑147 (MQ=255)                                                                                                            cgcgAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCg                                  >  4:144812/1‑147 (MQ=255)                                                                                                             gcgAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                 >  7:84753/1‑147 (MQ=255)                                                                                                                       gAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGt                        >  2:99239/1‑147 (MQ=255)                                                                                                                       gAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGt                        >  7:164487/1‑147 (MQ=255)                                                                                                                        aGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt                       <  3:356202/147‑1 (MQ=255)                                                                                                                        aGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt                       >  7:144340/1‑147 (MQ=255)                                                                                                                        aGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt                       >  8:150322/1‑147 (MQ=255)                                                                                                                         ggTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTg                      <  1:99239/147‑1 (MQ=255)                                                                                                                           tataGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGt                     >  1:123096/1‑146 (MQ=255)                                                                                                                              atcttccaTCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GAC‑AGGTGGCGGGGAGGGCTATCAGGCTTCCTGATAGCTCTCGATCGACTGGCAGGCGCACACCAGTTTGCTGTCTCCGAACACGTTGTCGa                  <  8:285578/141‑1 (MQ=255)                                                                                                                              aGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc                 >  7:41515/1‑147 (MQ=255)                                                                                                                              aGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc                 >  3:318224/1‑147 (MQ=255)                                                                                                                                 caccaTCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg              >  4:341301/1‑147 (MQ=255)                                                                                                                                 caccaTCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg              >  2:203823/1‑147 (MQ=255)                                                                                                                                 caccaTCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg              <  1:203823/147‑1 (MQ=255)                                                                                                                                 caccaTCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCg              <  8:74106/147‑1 (MQ=255)                                                                                                                                      tCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa         <  2:123096/147‑1 (MQ=255)                                                                                                                                      tCG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa         >  6:190265/1‑147 (MQ=255)                                                                                                                                       cG‑CATTCG‑C‑GGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc        <  1:54864/147‑1 (MQ=255)                                                                                                                                               g‑c‑gGG‑T‑AAACCCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcgg  <  8:81794/146‑1 (MQ=255)                                                                                                                                                            |                                                                                                                                             GGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCG‑CATTCG‑C‑GGG‑T‑AAA‑CCGCTCCTACACAG‑AG‑CCCGCCACCCAA‑GCC‑AGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGG  >  NC_002947/1126499‑1126781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑