Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,348,8910GA21.9% 61.0 / 10.9 34P366P (CCG→CCA) PP_2065multidrug RND transporter
Reads supporting (aligned to +/- strand):  ref base G (11/14);  new base A (7/0);  total (19/15)
Fisher's exact test for biased strand distribution p-value = 1.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCTATGAAACCGCACGCTTCATCGATGCGTCGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACGTGCACCGGCATATC  >  NC_002947/2348749‑2349032
                                                                                                                                              |                                                                                                                                             
ccTATGAAACCGCACGCTTCATCGATGCGTCGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTg                                                                                                                                            <  2:86393/146‑1 (MQ=255)
   aTGAAACCGCACGCTTCATCGATGCGTCGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGTTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGa                                                                                                                                        >  1:196178/1‑147 (MQ=255)
                  tCATCGATGCGTCGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCTCGGTGGTGGCCATTCCTCTGTAGATTCTGGGGGCTGCgg                                                                                                                         >  4:72487/1‑147 (MQ=255)
                   cATCGATGCGTCGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTCTGCCTGGGTTCGCTGCGCAGCGTGATGATCGCGGTGGTGGCCAATACGCTGTCGATGCTGTGGGCTGCggg                                                                                                                        >  3:257652/1‑147 (MQ=255)
                      cGATGCGTCGATGCATGAAGTGGTGCGCACCTTGGTTGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCt                                                                                                                     <  8:248902/147‑1 (MQ=255)
                              cGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGGCGATGCTGGGGGCTGCGGGCCtgatgttga                                                                                                             <  5:380660/147‑1 (MQ=255)
                                    aTGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGttt                                                                                                        <  7:289096/146‑1 (MQ=255)
                                     tGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGAAGATCTCGGTGGTCGCCATTACAATATAAAAAATAGGGGCTGCGGGCCTGATGTTGATGTTTgg                                                                                                      >  6:223333/1‑147 (MQ=255)
                                     tGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGCTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGAAGATCTCGGTGGTAGCCATAACAATATAAATAATACGGGCTGCGGGCCTGATGTTGATGTTTgg                                                                                                      >  8:288811/1‑147 (MQ=255)
                                          tgctgcGCACCTTGGTGGAGGCGTTGTTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCa                                                                                                 <  2:196178/147‑1 (MQ=255)
                                              gcgcTCCTTGGTGGAGGCGAGGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGGTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCt                                                                                             <  3:319059/147‑1 (MQ=255)
                                                 cACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGATGATCTCGGTGGTCGCCATTACAATAAATATAATTGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCaa                                                                                          >  2:301824/1‑147 (MQ=255)
                                                         tggAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGGTTCAGCCTCAACCTGCTGa                                                                                  <  3:72487/147‑1 (MQ=255)
                                                              gCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCTCTGCGCAGCGTGAAGATCTCGGTGGTAGCCATAACAATAAAAAAAAAAAGTAATGAGGGCCTGATGTTGATGTTTGGTTTCAGACTCAGCCTGCTGACCTTg                                                                             >  4:107828/1‑147 (MQ=255)
                                                              gCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGAAGATCTCGGTGGTCGCCATAACAATATAAAAAATAGGGGATGCGGGACTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGCCCTTg                                                                             >  4:99580/1‑147 (MQ=255)
                                                                 aTGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTg                                                                          <  4:120282/147‑1 (MQ=255)
                                                                               gtTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGAAGATCTCGGTGGTCGCCATAACAATAAAAAAAAAATAGGATGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGATgg                                                             >  6:15627/1‑146 (MQ=255)
                                                                                ttGGTGATCTGGCTGTGCCTTGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGcc                                                           <  2:300349/147‑1 (MQ=255)
                                                                                    tGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCg                                                       >  1:141237/1‑147 (MQ=255)
                                                                                    tGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCg                                                       >  5:294821/1‑147 (MQ=255)
                                                                                     gATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCgg                                                      <  7:90575/147‑1 (MQ=255)
                                                                                            cTGTGCCTGTGTTCGCTGCGCAGGGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGTGGGCCTGATGTTGATGTTTGGTTTCAGc                                                                                               <  5:74296/99‑1 (MQ=255)
                                                                                            cTGTGCCTGGGTTCGCTGCGCAGCGTGAAGATCTCGGTGGTCGCCATTACAATAAAAAAAATAGTGGCTGTGGGCCTGATGTTGATGTTTGGTTTCAGc                                                                                               >  6:74296/1‑99 (MQ=255)
                                                                                             tgtgCCTGGGTTCGCTGCGCAGCTTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCtggtgg                                              <  5:223333/147‑1 (MQ=255)
                                                                                                                    gTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTgtggtggtg                       <  2:141237/147‑1 (MQ=255)
                                                                                                                    gTGCTGATCGCGGTGCTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGACTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGACATCGGCCTGGTGGTGTACGATGCCATTgtggtggt                        >  5:273920/1‑146 (MQ=255)
                                                                                                                       cTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGa                     >  6:133158/1‑146 (MQ=255)
                                                                                                                          aTCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAAc                 >  3:65532/1‑147 (MQ=255)
                                                                                                                           tCGCGGTGGTGGCCATTCCTCTGTCGATTCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCGATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACg                <  7:288811/147‑1 (MQ=255)
                                                                                                                           tCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACg                >  4:194388/1‑147 (MQ=255)
                                                                                                                                   gtggCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACGTGCACCgg        <  6:294821/147‑1 (MQ=255)
                                                                                                                                        cATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACGTGCACCGGCatat   >  5:6333/1‑147 (MQ=255)
                                                                                                                                         aTTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACGTGCACCGGCATATc  >  7:96844/1‑147 (MQ=255)
                                                                                                                                          ttCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACGTGCACCGGCatat   >  5:284289/1‑145 (MQ=255)
                                                                                                                                              |                                                                                                                                             
CCTATGAAACCGCACGCTTCATCGATGCGTCGATCCATGAAGTGCTGCGCACCTTGGTGGAGGCGATGCTGATCGTGGTGTTGGTGATCTGGCTGTGCCTGGGTTCGCTGCGCAGCGTGCTGATCGCGGTGGTGGCCATTCCGCTGTCGATGCTGGGGGCTGCGGGCCTGATGTTGATGTTTGGTTTCAGCCTCAACCTGCTGACCTTGCTGGCGATGGTGCTGGCCATCGGCCTGGTGGTGGACGATGCCATTGTGGTGGTGGAAAACGTGCACCGGCATATC  >  NC_002947/2348749‑2349032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑