Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	143.4 / NA	41	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/18);  total (23/18)

AGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCG  >  NC_002947/4740680‑4740957                                                                                                                                         |                                                                                                                                               aGCCCGTACAAGATCGGTCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                     <  8:317178/147‑1 (MQ=255) aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGcc                                                                                                                                       <  6:80109/145‑1 (MQ=255) aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                     <  3:178464/147‑1 (MQ=255)    ccGTACAAGATCGGCCGTCCGCGCCAGCTATACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGAGCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCct                                                                                                                                  >  8:172372/1‑147 (MQ=255)           aGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGGAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                                           <  7:199680/147‑1 (MQ=255)               cGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑t                                                                                                                      <  5:1611/147‑1 (MQ=255)                 gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTg                                                                                                                    >  6:110365/1‑147 (MQ=255)                        gcgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAACTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCcgcg                                                                                                              <  4:295183/146‑1 (MQ=255)                         cgcCAGCTGTACACCGGAGATCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGATCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGaa                                                                                                            <  8:257101/147‑1 (MQ=255)                                tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCg                                                                                                     >  2:88314/1‑147 (MQ=255)                                tGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCC‑TTTCCCGCGAAGCGGCCg                                                                                                     >  7:153729/1‑147 (MQ=255)                                 gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCCGCC‑GTGCCCGCGAAGACGCCgg                                                                                                    >  5:221586/1‑147 (MQ=255)                                   acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGAGCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGca                                                                                                  <  7:172372/147‑1 (MQ=255)                                        ggCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGac                                                                                             >  6:323583/1‑147 (MQ=255)                                           gagcgtaaGCGCGACATGGTTGCCCTCAACGCCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCTAAGAGGCCGGCACAGAcacc                                                                                          <  2:240325/141‑1 (MQ=255)                                             gCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTGGCGGCC‑TGTGCCGCGAAGAGGCCGGGACAGccacca                                                                                         >  4:10235/1‑146 (MQ=255)                                                        aCATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCA‑TTGCCCCCGAAGAGGCCGGCACAGACACCACAAAACCTAAgg                                                                             >  7:285379/1‑146 (MQ=255)                                                                       aacaCCCCAAGTAAGCTCCAGTAGCCGAACCCAACAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGCCCTGTGCCGGCCTCTTCGCGCCC‑TTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                                <  8:92763/142‑1 (MQ=255)                                                                       aaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                                <  5:168232/145‑1 (MQ=255)                                                                       aaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATAGCCGCCATGGCCCGCGAA‑CGGCCGCCCCACACACCACAAAAACAGAATTTTTATACGCCCCCt                                                              >  8:312385/1‑147 (MQ=255)                                                                             gCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCa                                                        >  4:138033/1‑147 (MQ=255)                                                                             gCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTATACGCCCCCACACGCa                                                        >  4:155874/1‑147 (MQ=255)                                                                               aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAACAGGCCGGCACAGACACAACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                       >  1:45023/1‑146 (MQ=255)                                                                                 ttAAGCTGCATTAGCCGACCCCAACACGGCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCTATGAGGCCGGc                                                                                                   <  2:286973/99‑1 (MQ=25)                                                                                 gTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCC‑TTGCCCGCGAAGAGGCCGGc                                                                                                   >  1:286973/1‑100 (MQ=255)                                                                                     gcgccAGTTGCCGAACGCAATATGGGGGCCCTGGGGCAGCC‑TTTTTTATTTGTCCTGTGCGGGCCTTTTCGCGGCT‑TTGCCCGCTATGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                                <  6:221586/143‑1 (MQ=255)                                                                                      ctcaaGTACCCTAACGAAAGAAGGCTGCCCTCGGGCAG‑CATTTTTTATTTGCCCTGGGCCGCCCTCTTCGCGCCC‑TTGCCCGCTACGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                               <  3:21291/143‑1 (MQ=255)                                                                                      cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCCCGAAGAGGCCGGCACAGACCCCACCAAACCTCAGTTTTTCTCCGCCCGCGCACGCAGCCCTTTCa                                               >  5:314369/1‑147 (MQ=255)                                                                                       tgaaGTAGCCGAAGCCAAGACGGCTGCCCTCGGCCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGAGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                              <  3:322874/144‑1 (MQ=255)                                                                                       gtcAGTACGCGAACGGAAGAAGCCTGCCCTCGTGCTGCCTTTTTTT‑TTTGTCCTGTGCCGGCCGCTTCGCGGCC‑TTGCCCGCGATTAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                              <  7:70127/145‑1 (MQ=255)                                                                                         cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                             >  3:265768/1‑146 (MQ=255)                                                                                         cAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                            <  3:236011/147‑1 (MQ=255)                                                                                                        agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCAt                             >  3:281478/1‑147 (MQ=255)                                                                                                           agGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca                          >  1:256314/1‑147 (MQ=255)                                                                                                             gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                        >  2:62234/1‑147 (MQ=255)                                                                                                                  ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGaa                   >  6:354193/1‑147 (MQ=255)                                                                                                                     agggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑ATGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt                >  2:41382/2‑147 (MQ=255)                                                                                                                       ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt                <  5:110365/145‑1 (MQ=255)                                                                                                                              cttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTTCCTGCGAAGCGGCCGGCACAGACACCACAAAACCTCAGGTTTTCTACGCCCGCGCACGCAGCCCGTTAAGGGTATTTAACGGCGCATCCACCACGAACATTTTTGcca        >  4:181561/2‑147 (MQ=255)                                                                                                                              cttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCCCCACGAACGTGTTGGcca        >  8:94144/2‑147 (MQ=255)                                                                                                                                    tATTTGTCCTGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCg  >  8:83629/1‑147 (MQ=255)                                                                                                                                         |                                                                                                                                               AGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCC‑TTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCATCG  >  NC_002947/4740680‑4740957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑