Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	92.6 / NA	26	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  NC_002947/499070‑499330                                                                                                                                      |                                                                                                                                 aCCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGa                                                                                                                     <  7:30036/147‑1 (MQ=255)    cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCGTGCCCGCGAACg                                                                                                                  >  4:159939/1‑147 (MQ=255)    cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACg                                                                                                                  >  6:385507/1‑147 (MQ=255)        aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcc                                                                                                              <  5:153375/147‑1 (MQ=255)           cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCCGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGGCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg‑g                                                                                                          >  6:243067/1‑147 (MQ=255)             cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctg‑gcc                                                                                                        >  8:24346/1‑147 (MQ=255)                    tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGa                                                                                                  <  6:288470/146‑1 (MQ=255)                    tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGAt                                                                                                 >  2:56215/1‑147 (MQ=255)                    tGCAGAAGTTGAAAGTGAACTCGATGGCGTAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTCGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGa                                                                                                  <  2:250174/146‑1 (MQ=255)                      cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCGGAtct                                                                                               >  5:351319/1‑147 (MQ=255)                       aGAAGTTGAAAGTGAACTCGCTGGCCGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGGCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGAtctc                                                                                              <  5:243067/147‑1 (MQ=255)                         aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctg‑gcctg                                                                                                      <  3:2523/137‑1 (MQ=255)                         aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGgcctg‑gcctg                                                                                                      >  4:2523/1‑137 (MQ=255)                         aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTa                                                                                            <  1:333775/147‑1 (MQ=255)                            ttGAAAGTGAACTCGCTGGCGGAGCTGGTGTAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCGCTATgg                                                                                         >  2:310456/1‑147 (MQ=255)                            atGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCCTGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATgg                                                                                         <  4:45939/146‑1 (MQ=255)                                aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTg                                                                                      <  5:241476/146‑1 (MQ=255)                                aaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTg                                                                                      <  1:56215/146‑1 (MQ=255)                                      aCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTGAGCCgg                                                                                <  5:385507/146‑1 (MQ=255)                                          gCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAATCCCGCGGACGGGCCAG‑GGCAGGTGATCTATATTGCTTAGGCGGCctc                                                                            >  5:201387/1‑146 (MQ=255)                                           cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCTTCCCGCGAACGGGGCGG‑GGCGGCGGATATGTATTGGTGTGCCGGccgc                                                                            >  3:180284/1‑143 (MQ=255)                                               cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTcgcg                                                                      <  6:236791/147‑1 (MQ=255)                                                                            aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCCCGCACGGGCCTG‑GCCAGCGGTTCTCTATTGGTGAGCCCGGCACTTAGCGGGCCAGCCCGCGCCCCCAGGGTTAGTGGt                                         >  5:1986/1‑147 (MQ=255)                                                                                            gACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTAGTGGTTGGCTCGCAAGGGCAt                         >  2:234362/1‑147 (MQ=255)                                                                                             aCATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCAtt                        >  6:188615/1‑147 (MQ=255)                                                                                                                     gAAGCCTTTGTGGGTGCGGGCATGCCCGCGAACGGGCCTGTTACCCATTATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:131181/146‑1 (MQ=37)                                                                                                                                      |                                                                                                                                 ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTG‑GCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  NC_002947/499070‑499330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑