Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	307,866	+C	intergenic (+881/‑988)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	239.5 / NA	70	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (37/33);  total (37/33)

GGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCG  >  NC_002947/307731‑308001                                                                                                                                         |                                                                                                                                           ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCg                                                                                                                               >  6:363531/1‑149 (MQ=255)        aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑c                                                                                                                                           <  8:3872/129‑1 (MQ=255)        aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑c                                                                                                                                           >  7:3872/1‑129 (MQ=255)               tgctgcCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCAc                                                                                                                 >  6:408267/1‑149 (MQ=255)                   gccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGtt                                                                                                             >  8:38578/1‑149 (MQ=255)                    ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                            <  7:49773/149‑1 (MQ=255)                    ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                            >  4:158412/1‑149 (MQ=255)                       gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                         <  6:389356/149‑1 (MQ=255)                           ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCg                                                                                                       <  7:38578/147‑1 (MQ=255)                              aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc                                                                                                  >  7:33251/1‑149 (MQ=255)                                  aCTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                               <  8:212599/148‑1 (MQ=255)                                   cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                             <  3:158412/149‑1 (MQ=255)                                    tGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTAGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                             >  1:115066/1‑149 (MQ=255)                                      ccTATGCACTGCGCGGTCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑t                                                                                          <  2:271085/148‑1 (MQ=255)                                      ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATTGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                            <  8:44940/147‑1 (MQ=255)                                      ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACgg                                                                                                               <  3:276510/128‑1 (MQ=255)                                      ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACgg                                                                                                               >  4:276510/1‑128 (MQ=255)                                      ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACACTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑tt                                                                                         >  4:144041/1‑149 (MQ=255)                                       cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTc                                                                                        <  1:276816/149‑1 (MQ=255)                                       cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTc                                                                                        <  5:363531/149‑1 (MQ=255)                                          tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAc                                                                                     <  3:144041/149‑1 (MQ=255)                                          tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAc                                                                                     <  3:171118/149‑1 (MQ=255)                                            cACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑At                                                                                  >  5:242728/1‑149 (MQ=255)                                             aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                            <  6:15354/140‑1 (MQ=255)                                             aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                            >  5:15354/1‑140 (MQ=255)                                                 cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGaa                                                                              <  5:408267/148‑1 (MQ=255)                                                          cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGgctgc                                                                     >  2:199408/1‑148 (MQ=255)                                                                   agaagGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGt                                                           <  2:115066/149‑1 (MQ=255)                                                                       ggCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCAt                                                       >  1:146685/1‑149 (MQ=255)                                                                         cGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc                                                                                                  >  2:49426/1‑106 (MQ=255)                                                                         cGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc                                                                                                  <  1:49426/106‑1 (MQ=255)                                                                                aCCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTc                                              >  4:153547/1‑149 (MQ=255)                                                                                  cTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                             >  7:232264/1‑148 (MQ=255)                                                                                  cTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                             >  7:238490/1‑148 (MQ=255)                                                                                   ttGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                   >  2:252748/1‑141 (MQ=255)                                                                                   ttGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCGGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAACAATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                   <  1:252748/142‑1 (MQ=255)                                                                                   ttGTTCCTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                             >  4:288061/1‑147 (MQ=255)                                                                                      ttcctcGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga                                         <  3:288061/148‑1 (MQ=255)                                                                                         ttcGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                     >  2:383215/1‑149 (MQ=255)                                                                                           cGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                   <  3:102256/133‑1 (MQ=255)                                                                                           cGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                   >  4:102256/1‑133 (MQ=255)                                                                                           cGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga                                      <  4:200714/146‑1 (MQ=255)                                                                                              ccT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc                                >  7:41824/1‑149 (MQ=255)                                                                                                t‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                              >  8:244326/1‑149 (MQ=255)                                                                                                   tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                     <  5:129734/140‑1 (MQ=255)                                                                                                   tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                     >  6:129734/1‑140 (MQ=255)                                                                                                   tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGt                            >  2:150953/1‑149 (MQ=255)                                                                                                          cTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTgg                     <  8:33251/149‑1 (MQ=255)                                                                                                                   cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc            <  8:41824/149‑1 (MQ=255)                                                                                                                      cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCg                                                                                                 >  6:244888/1‑63 (MQ=255)                                                                                                                      cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCg                                                                                                 <  5:244888/63‑1 (MQ=255)                                                                                                                      cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg         >  5:217755/1‑149 (MQ=255)                                                                                                                       ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGa                                  >  2:306238/1‑123 (MQ=255)                                                                                                                       ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGa                                  <  1:306238/123‑1 (MQ=255)                                                                                                                       ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg         >  3:402210/1‑148 (MQ=255)                                                                                                                         tttCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                       >  5:83867/1‑132 (MQ=255)                                                                                                                         tttCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                       <  6:83867/132‑1 (MQ=255)                                                                                                                            cGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                       >  6:27679/1‑129 (MQ=255)                                                                                                                            cGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                       <  5:27679/129‑1 (MQ=255)                                                                                                                            cGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACgg    <  6:217755/148‑1 (MQ=255)                                                                                                                             ggCTGACGAAAGCCGTTACCTGATCGGTGACGACGATCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAA‑ATGGaa                                                                              >  6:206513/1‑73 (MQ=255)                                                                                                                             ggCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGTGGAGAAGGCGTTGTC‑TTCAAA‑ATGGaa                                                                              <  5:206513/73‑1 (MQ=255)                                                                                                                              gCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa          >  6:394023/1‑140 (MQ=255)                                                                                                                              gCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa          >  6:388142/1‑140 (MQ=255)                                                                                                                              gCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa          <  5:394023/140‑1 (MQ=255)                                                                                                                              gCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCaa          <  5:388142/140‑1 (MQ=255)                                                                                                                              gCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGgcg  >  7:347863/1‑148 (MQ=255)                                                                                                                                tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGgcg  >  1:115798/1‑147 (MQ=255)                                                                                                                                    gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg                             >  7:5525/1‑115 (MQ=255)                                                                                                                                    gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCgg                             <  8:5525/115‑1 (MQ=255)                                                                                                                                    gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc            >  8:337457/1‑132 (MQ=255)                                                                                                                                    gAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc            <  7:337457/132‑1 (MQ=255)                                                                                                                                         |                                                                                                                                           GGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCT‑GTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTC‑TTCAAC‑ATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCG  >  NC_002947/307731‑308001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑