Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,419,549	+C	intergenic (‑38/+98)	PP_1242 ← / ← PP_1244	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,419,548	1	.	C	100.0%	170.8 / NA	51	intergenic (‑37/+99)	PP_1242/PP_1244	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (27/24);  total (27/24)

CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTG  >  NC_002947/1419401‑1419671                                                                                                                                                      |                                                                                                                            gtGGCAGGGGTTGGCGGTTTCGATGGGGAACTGCGGGTGAGTGGTGGTTGGGCGTTGGTAGAAGCTGGTCTTGCCGATTGTGGTGGGTTCTGTTG‑TGTTGTCGTTGGGCATTGTTTTGTTTCGCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                            <  7:282341/148‑1 (MQ=255) cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                            >  8:113397/1‑149 (MQ=255)          gATGGCGGGTTCTATGCGGCACAGCGGGGGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGAGTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTcgcg                                                                                                                    <  6:308339/149‑1 (MQ=255)            tGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCggg                                                                                                                  >  7:85782/1‑149 (MQ=255)               cGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                            >  1:274056/1‑135 (MQ=255)               cGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                            <  2:274056/135‑1 (MQ=255)                 ggTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGc                                                                                                             <  7:113397/149‑1 (MQ=255)                       aTGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTGTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGcc                                                                                                            >  6:334343/1‑144 (MQ=255)                       aTGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGcc                                                                                                            <  5:334343/144‑1 (MQ=255)                        tGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTCGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTcc                                                                                                      <  3:243256/149‑1 (MQ=255)                          cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGcc                                                                                                            <  1:59339/141‑1 (MQ=255)                          cGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGcc                                                                                                            >  2:59339/1‑141 (MQ=255)                           ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCca                                                                                                    >  6:183155/1‑148 (MQ=255)                           ggAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCca                                                                                                    >  4:333227/1‑148 (MQ=255)                                     gTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAgggtcacca                                                                                         >  8:322255/1‑142 (MQ=255)                                       gAGTGTGGGTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTGG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCa                                                                                       <  6:48027/149‑1 (MQ=39)                                          tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                                                  <  8:227374/135‑1 (MQ=255)                                          tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCcaca                                                                                                  >  7:227374/1‑135 (MQ=255)                                           gttcttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGCGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTACCACACGTACTGTCCAGTGc                                                                                   >  7:50188/1‑149 (MQ=255)                                              gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACGGTGCAGTGCGTg                                                                                >  2:305322/1‑149 (MQ=255)                                               ttttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                               >  5:89854/1‑149 (MQ=255)                                                tttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGAc                                                                              >  4:352257/1‑149 (MQ=255)                                                tttCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCATGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGATACCACAGGTACTGTCCAGTGCGTGAc                                                                              >  2:128110/1‑149 (MQ=255)                                                      cTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTg                                                                                    >  2:354798/1‑137 (MQ=255)                                                      cTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTg                                                                                    <  1:354798/137‑1 (MQ=255)                                                      cTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgt                                                                        >  3:300029/1‑149 (MQ=255)                                                       ttGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACgg                                                                            <  8:152470/144‑1 (MQ=255)                                                       ttGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACgg                                                                            >  7:152470/1‑144 (MQ=255)                                                          gTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtggt                                                                     <  4:92303/148‑1 (MQ=255)                                                              aatcatgTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAcc                                                                 <  1:128110/142‑1 (MQ=255)                                                                gCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAc                                                                  <  8:20191/145‑1 (MQ=255)                                                                gCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAc                                                                  >  7:20191/1‑145 (MQ=255)                                                                   ggTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTgg                                                           <  4:300029/149‑1 (MQ=255)                                                                     tCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTc                                                                                                                       <  5:44350/87‑1 (MQ=255)                                                                     tCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTc                                                                                                                       >  6:44350/1‑87 (MQ=255)                                                                      cTTGCCGACTGTGGTGGGTTCTGTTGTTGTTGTCGGTGGGAATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACCCCCGCTCCCACAGGTACTGTGCAGTGAGTGACGGGTTTGGTTACCCTGTgg                                                           >  1:222810/1‑147 (MQ=38)                                                                       ttGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCt                                                                                                        <  1:380154/100‑1 (MQ=255)                                                                       ttGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCt                                                                                                        >  2:380154/1‑100 (MQ=255)                                                                          ccGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCGCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCggg                                                    <  6:89854/149‑1 (MQ=255)                                                                          ccGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCgg                                                     <  3:352257/148‑1 (MQ=255)                                                                                      ggTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtgg                                                                      >  5:58497/1‑119 (MQ=255)                                                                                      ggTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTgtgg                                                                      <  6:58497/119‑1 (MQ=255)                                                                                        ttCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCGGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAaga                                      <  3:333227/149‑1 (MQ=255)                                                                                         tCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGGGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAagag                                     <  5:183155/149‑1 (MQ=255)                                                                                               g‑tgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGa                                                                   >  2:167736/1‑113 (MQ=255)                                                                                               g‑tgttgtCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGa                                                                   <  1:167736/113‑1 (MQ=255)                                                                                                            ggCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGc                                               <  2:238383/121‑1 (MQ=255)                                                                                                            ggCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTACCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAACCTGTGGGAGCGGGCGTGc                                               >  1:238383/1‑121 (MQ=255)                                                                                                              cATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGGGTGCCCGCGCAGAGGCCAGACCTGCTAAAGCAAg                 >  4:40935/1‑149 (MQ=37)                                                                                                              cATTGTTTTGTTCCCCTGGATGCTGGGTGGCGGGGGCTGGCCTCTGCGCGGGCCCGCCCGCTCCCACCGGTACTGTGCAGTGCGTGACGGCGGTGGTGCCCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAg                 <  1:305322/149‑1 (MQ=37)                                                                                                                             cTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCGGTGGGCGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGTGGCTCtgtg  >  5:317760/1‑149 (MQ=37)                                                                                                                             cTGGATTCTGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCAAAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCGGACCTGCTAATGCAAGGTGTGAGGCtgtgtg  >  5:116/1‑149 (MQ=37)                                                                                                                                     tGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGAc                              <  7:127171/113‑1 (MQ=255)                                                                                                                                     tGGGTTGCCTGGGCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGAc                              >  8:127171/1‑113 (MQ=255)                                                                                                                                               gggCTGGCCTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGGAGTGCGTGACGGCTGTGGTGGCGCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGCCCTGCTAATGCAAGGTGTGAGGCTCtgtg  >  5:93898/1‑131 (MQ=25)                                                                                                                                                      |                                                                                                                            CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGAGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTG‑TGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑CTCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTG  >  NC_002947/1419401‑1419671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑