Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,287,2760TC26.9% 33.2 / 14.1 27intergenic (+17/+24)PP_2889/xthAtransmembrane regulator PrtR/exodeoxyribonuclease III/apurinic/apyrimidinic endodeoxyribonuclease VI
Reads supporting (aligned to +/- strand):  ref base T (13/6);  new base C (5/2);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCCAGCTGGAGGCGCGGTACTGGTCGCATGGGGCTTATAACTTTGCCTTGGT‑CAGCGCGGCGGACGATGTGCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGATCAGGTCGATGC  >  NC_002947/3287145‑3287424
                                                                                                                                    |                                                                                                                                                     
tGGCCAGCTGGAGGCGCGGTACTGGTCGCATGGGGCTTATAACTTTGCCTTGGTACAGCGCGGCGGACGATGTGCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGt                                                                                                                                       >  1:307333/1‑148 (MQ=255)
   ccAGCTGGAGGCGCGGTACTGGTCGCATGGGGCTTATAACTTTGCCTGGGT‑CAGCGCGGCGGACGATGTGCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCAccc                                                                                                                                  >  1:291859/1‑149 (MQ=255)
       cTGGAGGCGCGGTACTGGTCGCATGGGGCTTATAACATTGCCTTGGT‑CAGCGCGGGGCACGATGTGCGAGGGGCGGGGGTGGGGGAGGACTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACCTTTGGGccctgt                                                                                                                                      >  5:68835/1‑139 (MQ=255)
            ggCGCGGTACTGGTCGCATGGGGCTTATAACTTTGCCTTGGT‑CAGCGCGGCGGACGATGTGCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCt                                                                                                                         >  1:223677/1‑149 (MQ=255)
                        gTCGCATGGGGCTTATAACTTTGCCTTGGT‑CAGCGCGGCGGACGATGTGCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTccagc                                                                                                               >  2:187228/1‑147 (MQ=255)
                                                                         gCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTc                                                             >  2:71721/1‑149 (MQ=255)
                                                                                  gggggTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATACCGCGCAGCTCATAGTCCACGCCCGc                                                    >  6:303552/1‑149 (MQ=255)
                                                                                        gggggAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCtt                                              >  6:74721/1‑149 (MQ=255)
                                                                                         ggggAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTg                                             <  5:74721/149‑1 (MQ=255)
                                                                                                       gTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAg                               <  2:223677/149‑1 (MQ=255)
                                                                                                             gTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGctgctg                         >  8:54835/1‑149 (MQ=255)
                                                                                                               tGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCtgtg                       >  5:26278/1‑149 (MQ=255)
                                                                                                               gggaaGTGTGGGCCCAAGGTGGGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGAGCGGCGCATGGGCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCtgtg                       <  6:132081/146‑1 (MQ=255)
                                                                                                                gTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCtgt                        >  7:216291/1‑147 (MQ=255)
                                                                                                                       tgGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCAt                <  5:303552/148‑1 (MQ=255)
                                                                                                                        gggCCCAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGa                      <  2:97203/141‑1 (MQ=255)
                                                                                                                        gggCCCAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGa                      >  1:97203/1‑141 (MQ=255)
                                                                                                                          gCCCAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGag             >  7:252881/1‑147 (MQ=255)
                                                                                                                           cccAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAgg                    <  7:246506/140‑1 (MQ=255)
                                                                                                                           cccAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAgg                    >  8:246506/1‑140 (MQ=255)
                                                                                                                           cccAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGATCa           <  8:216291/149‑1 (MQ=255)
                                                                                                                           cccAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTg                                             >  6:386371/1‑115 (MQ=255)
                                                                                                                           cccAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTg                                             <  5:386371/115‑1 (MQ=255)
                                                                                                                           cccAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGAt             >  3:12659/1‑147 (MQ=255)
                                                                                                                           cccAAGGTGCACACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGAt             >  5:214453/1‑147 (MQ=255)
                                                                                                                              aaGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAg                     >  3:196525/1‑136 (MQ=255)
                                                                                                                              aaGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAg                     <  4:196525/136‑1 (MQ=255)
                                                                                                                                    tgCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGATCAGGTCGATgc  >  2:398488/1‑149 (MQ=255)
                                                                                                                                    |                                                                                                                                                     
TGGCCAGCTGGAGGCGCGGTACTGGTCGCATGGGGCTTATAACTTTGCCTTGGT‑CAGCGCGGCGGACGATGTGCGTGGGGCGGGGGTGGGGGAGGCGTTGAGGTTGGGGTTGTAAGTGTGGGCCCAAGGTGTGCACC‑TTGGGCCCGTCACCCTAATCAGCTCAACTCCAGCCAGATCGGCGCATGGTCCGACGGCTTTTCCATCCCGCGCAGCTCATAGTCCACGCCCGCTGCCTTGATGCGCGGCACCAGCTGCTGTGAGGCCATGATCAGGTCGATGC  >  NC_002947/3287145‑3287424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑