Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	127.5 / NA	40	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/20);  total (20/20)

GTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740685‑4740952                                                                                                                                    |                                                                                                                                          gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                         >  7:50114/1‑149 (MQ=255) gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc                                                                                                                         <  2:59853/149‑1 (MQ=255)    cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTcgcg                                                                                                                      <  2:200504/149‑1 (MQ=255)                  cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                        <  3:174487/149‑1 (MQ=255)                  cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGa                                                                                                         <  5:206851/148‑1 (MQ=255)                    cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                      <  2:216192/149‑1 (MQ=38)                    cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAaga                                                                                                      <  3:208706/149‑1 (MQ=38)                     gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                     <  3:35631/149‑1 (MQ=255)                          cTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                     >  1:357255/1‑144 (MQ=255)                          cTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAagag                                                                                                     <  2:357255/144‑1 (MQ=255)                               cacCGGCGAGCAGAAGCGCGACATCGTTGCCATGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTAAGCGGCCTTGACCTCGAACAGGC‑CAGcaca                                                                                           >  4:269168/1‑148 (MQ=255)                                acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGa                                                                                         >  3:112555/1‑149 (MQ=255)                                  cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                      >  6:154188/1‑135 (MQ=255)                                  cGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAaga                                                                                                      <  5:154188/135‑1 (MQ=255)                                           gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                               <  5:343391/101‑1 (MQ=255)                                           gAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                               >  6:343391/1‑101 (MQ=255)                                                           gCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTccgc                                                              >  1:69639/1‑149 (MQ=255)                                                                gAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                         <  1:11435/149‑1 (MQ=255)                                                                       cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACCCCACAAAACCTCAGTTTTTCTACGCCCGCTCCCGCAg                                                  >  2:130155/1‑149 (MQ=255)                                                                       cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                     >  6:210687/1‑146 (MQ=255)                                                                       cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                     <  5:210687/146‑1 (MQ=255)                                                                       cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑AGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                  >  1:54614/1‑149 (MQ=255)                                                                                 cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                        <  2:69639/149‑1 (MQ=255)                                                                                  tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCATGCCCGCGAAGAGGC‑CGGCAAAGACACCACAAAACCTCAGTTTTTCTCCGCCCCCTCACGCAGCCCTTTCAgg                                        >  7:284374/1‑148 (MQ=255)                                                                                      gaaGCCCAACGCAAGAAGGCGGCCCTCGGCCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAtt                                   <  4:362969/147‑1 (MQ=255)                                                                                          ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                               >  2:81722/1‑149 (MQ=255)                                                                                              aCGCTAGAAGGGTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCATTGCCGGCCTCTTCGCGGCCTTGCCCGCGAATAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                            <  3:269168/148‑1 (MQ=255)                                                                                                  aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                       <  1:81722/149‑1 (MQ=255)                                                                                                  aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTAGCCGCCGTTGCCGCGCAGAGGC‑CCGCCCACACACCACAAAACCCCCCATTTTGTCCGCCCCCACACCCCCCCCCTTCAGGGGATTTTAACGCGCATc                       >  2:212885/1‑149 (MQ=255)                                                                                                  aagaacGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCATCTTAGATGCCTAGCCCCCCAAGAACC‑CGGCACAAACAACCAAAAACCACAGTATTTCTAAGCACCCTCAAGCAGACCATTAAAGGTATTAAACCGCGCaaa                       >  2:16067/1‑147 (MQ=255)                                                                                                       ggCTGCCCGAGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTTCCCGCGAAGAGGC‑Cgg                                                                                               >  1:1978/1‑72 (MQ=18)                                                                                                        gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                     >  5:315869/1‑113 (MQ=38)                                                                                                        gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                     <  6:315869/113‑1 (MQ=38)                                                                                                        gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCGCTTCGCGGCCATGCCCGCGAAGAGGC‑CCGCCCAGACCCCACCAAACCACAGTTTTTATCCGCCCGCTCCCGCAGCCCGTTCACGGTATTGAACGGCGCAGCcaccac                 >  4:80618/1‑149 (MQ=255)                                                                                                             ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                         <  7:281741/136‑1 (MQ=39)                                                                                                             ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCa                         >  8:281741/1‑136 (MQ=39)                                                                                                                 gggCTGCC‑TTTTTTATTTGCCCTGTGCGGGCCTCTTCGCGGCGTGGCCCGCTATGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt        <  8:50114/149‑1 (MQ=255)                                                                                                                 gggCAGCC‑TTTTTTATTTGTCCTGTGCCGCCCTATTCGCGGCGTTGCCCGCTAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttg          <  7:49630/147‑1 (MQ=255)                                                                                                                        ccttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGGGGC‑CGGGACAGACACCACAAAAc                                                                              >  7:145787/3‑73 (MQ=25)                                                                                                                          ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCGCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCCCGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac  >  1:100501/1‑148 (MQ=255)                                                                                                                                    |                                                                                                                                          GTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGC‑CGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740685‑4740952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑