| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 5,674,753 | G→C | intergenic (+115/+25) | rhlE‑II → / ← yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,674,753 | 0 | G | C | 98.3% | 194.0 / NA | 58 | intergenic (+115/+25) | rhlE‑II/yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (26/31); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTT > NC_002947/5674612‑5674885 | ccAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCg < 2:358696/149‑1 (MQ=255) cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCgg < 1:24067/149‑1 (MQ=255) aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCt < 7:372049/147‑1 (MQ=255) cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGc < 5:53651/149‑1 (MQ=255) ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTgg > 2:366224/1‑147 (MQ=255) cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGtt > 5:343734/1‑137 (MQ=255) cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGtt < 6:343734/137‑1 (MQ=255) cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGaa < 3:69751/149‑1 (MQ=255) tGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATATCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACtt > 5:272567/1‑149 (MQ=255) gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCC‑GCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCAAACCt > 8:126878/1‑149 (MQ=255) tggggCTGCTTCTCAGCCCTATAACGAGACTAGGGCGCTACTGGAGAGATTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGTCCGTAAAG‑CGGCGCCCTCGGTTTTCAGCTGGCAGATATTACTTCTGGTTcagtggtt < 4:362032/147‑7 (MQ=255) gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc < 2:37198/149‑1 (MQ=255) gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa < 3:286591/149‑1 (MQ=255) gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca < 5:45126/149‑1 (MQ=255) tgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt > 3:289921/1‑149 (MQ=255) ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACt < 7:372965/147‑1 (MQ=255) ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTg < 5:365025/148‑1 (MQ=255) gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCt > 4:330499/1‑108 (MQ=255) gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCt < 3:330499/108‑1 (MQ=255) aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGGTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc > 3:4741/1‑147 (MQ=255) aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCCCAAAT‑CGGCCCCCGCGGTTTTGAGCTGGCAGAAATTAATTATGCATTACAACATAGACACAGATAtcca > 2:400803/1‑149 (MQ=25) gCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccag < 4:289921/149‑1 (MQ=255) cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag < 8:312617/125‑4 (MQ=25) cccTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag > 7:312617/1‑122 (MQ=25) aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa < 6:98147/131‑1 (MQ=255) aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa > 5:98147/1‑131 (MQ=255) aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGGTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg > 1:327757/1‑149 (MQ=255) cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGt < 2:327757/149‑1 (MQ=255) cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg > 6:290896/1‑143 (MQ=255) cGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg < 5:290896/143‑1 (MQ=255) cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAccc > 8:56664/1‑149 (MQ=255) gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc < 7:386497/107‑1 (MQ=255) aaTTTGTTGATCTCTACAGGAGCGGCGTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCACTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCtt < 7:126878/148‑1 (MQ=255) aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt < 1:277731/148‑1 (MQ=255) ttGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc > 1:320814/1‑149 (MQ=255) gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg < 1:366224/148‑1 (MQ=255) tctACAGGAGCGGCCTTGTGTCGTGATAGGGACGCAAAG‑CGGCCACCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATACACGTCAAGGGTCTGCGAAGTAGCACCCCGGCCTTTGATGCCGAAGTc > 6:361670/1‑149 (MQ=255) ctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCg > 8:35632/1‑149 (MQ=255) aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac < 5:299741/91‑1 (MQ=255) aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac > 6:299741/1‑91 (MQ=255) aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt > 6:27446/1‑149 (MQ=255) aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt > 3:27435/1‑149 (MQ=255) gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg < 2:320814/149‑1 (MQ=255) gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg < 7:35632/149‑1 (MQ=255) ttGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt < 2:55052/137‑1 (MQ=255) ttGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt > 1:55052/1‑137 (MQ=255) tgtCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt > 1:8933/1‑149 (MQ=255) tgtCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtgg < 4:171315/148‑1 (MQ=255) tCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc < 7:238485/122‑1 (MQ=255) tCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGc > 8:238485/1‑122 (MQ=255) tCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg > 7:231499/1‑149 (MQ=255) gTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg > 1:61983/1‑149 (MQ=255) aTAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc < 6:272567/148‑1 (MQ=255) gggCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt < 2:8933/148‑1 (MQ=255) ggCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCg > 2:260208/1‑134 (MQ=255) ggCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCg < 1:260208/134‑1 (MQ=255) ccGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt < 7:122178/145‑1 (MQ=255) ccGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt > 8:122178/1‑145 (MQ=255) | CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTT > NC_002947/5674612‑5674885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |