| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,586,030 | 2 bp→TC | intergenic (+113/+101) | PP_4061 → / ← PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,586,030 | 0 | C | T | 97.1% | 100.6 / ‑4.2 | 34 | intergenic (+113/+102) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (13/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
| * | NC_002947 | 4,586,031 | 0 | T | C | 97.0% | 101.0 / ‑5.6 | 34 | intergenic (+114/+101) | PP_4061/PP_4063 | hypothetical protein/long‑chain fatty acid‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (12/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAG‑GGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAA > NC_002947/4585897‑4586110 || gAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGc < 4:103633/149‑1 (MQ=255) gCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTc < 2:36550/149‑1 (MQ=255) gAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATTACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAg‑g > 3:227536/1‑148 (MQ=39) aaGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGa > 2:96197/1‑149 (MQ=255) aGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGA‑c < 6:130049/149‑1 (MQ=255) cTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTCTTCTCG‑GGTAAACCCGCTCCTACCAG‑GGA‑ccgt < 4:227536/149‑4 (MQ=39) ttAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGA‑ccgtg > 8:9597/1‑145 (MQ=39) aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGcc > 5:397941/1‑149 (MQ=39) aaGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGA‑ccgtgc < 6:392321/148‑6 (MQ=39) aGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCg < 3:119332/149‑1 (MQ=39) aCGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATg < 2:340909/149‑1 (MQ=39) tGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGcccc < 7:9597/149‑1 (MQ=39) gAACAGCTGATCTGCGCTGCTCCATTGAAAGGCTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCt > 3:386500/1‑149 (MQ=38) aaCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg > 8:72959/1‑149 (MQ=39) aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGATCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg < 5:394608/149‑1 (MQ=39) aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTgg < 1:96197/149‑1 (MQ=39) aCAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTg < 3:106355/148‑1 (MQ=39) cAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 6:397941/149‑1 (MQ=39) tGCGCTGCTCCATTGAAAGGTTGAGGATGACCGGGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt > 1:48210/1‑141 (MQ=38) tGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGt < 2:48210/140‑1 (MQ=38) cgcTGCTCCATTGAAAGGCTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg < 4:386500/146‑1 (MQ=38) gctgctCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGttt < 7:72959/149‑1 (MQ=38) ctCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 1:60507/147‑1 (MQ=38) ccATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAc > 7:363823/1‑145 (MQ=38) ccATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTAc < 8:363823/145‑1 (MQ=38) cATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcg < 7:41479/147‑1 (MQ=38) cATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCGTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCcg > 8:41479/1‑147 (MQ=38) cATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATAGATCGGGCCGGCCTC‑TTCGCGGGTTAAAACCCTCCTAGCAA‑GGACCGGGCAGGCCATGAAATCGGCGCGGCCCCAGGGAAGAGCGGGGTTAACagcg > 1:303172/1‑149 (MQ=255) tttaaaGGTTGTGGATGACC‑GGGTGCTTGGCAAGGTGAACATCTTGCATTGATCGGCCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCCG‑GGACCGTGCCGGCCTTTAATGCGGCGCTGCCCCTGGTAGGTGCGGTTTTACCCGCGaa < 4:123960/146‑1 (MQ=37) ttGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg > 2:36351/1‑136 (MQ=38) ttGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg < 1:36351/136‑1 (MQ=38) ttGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTA‑TTAGCGGGTAAAACCGCAACAACCAGTGCC‑CGGGCCGGCCTTGCATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTTACCGCGaa > 8:300616/1‑149 (MQ=255) tGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg > 2:230373/1‑127 (MQ=38) tGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TTCGCGGGTAAACCCGCTCCTACCAG‑GGACCGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCgg < 1:230373/127‑1 (MQ=38) || GAGCCACCGGTGAAGGCTTAAGGGTAGACCACGGCACCGGCGTGAACAGCTGATCTGCGCTGCTCCATTGAAAGGTTGAGGATGACC‑GGGTGCTTGGCAAGGTGAAGATCTTGCATTGATCGGGCCGGCCTC‑TCTGC‑GGTAAACCCGCTCCTACCAG‑GGA‑CGTGCCGGCCTTGAATGCGGCGCTGCCCCTGGTAGGAGCGGGTTTACCCGCGAA > NC_002947/4585897‑4586110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |