| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 92.5% | 127.6 / 5.2 | 41 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (16/21); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932497‑1932779 | cgTCTTCGATGAGTATCTGGACATTCGACAGCGGCATTGCAGGTCTCACTAAAATAGCATTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑c‑ccgc < 6:13526/147‑4 (MQ=38) cttggATCTGTACATTCTACAGCGCCATTCCAGGTCTCACTAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGC‑ATATGCGTCCCCTTTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCt < 8:387912/143‑1 (MQ=37) aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TTGG‑C‑CGCGAAGGGCTGCAAGGc > 8:215507/1‑148 (MQ=255) aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GAC‑CGCGACGGGCTGCAAgg > 8:209875/1‑147 (MQ=255) tcATTCGACAGGGGCTTTGCAGGTCTCCCGAAAATCGCAGTAAACGCGGGTGCCGCTTTGCGGCCCTTCGCGGGCACGCCGGCTCATACAACGG‑ATATGCGTCCCCTGTAGGAGGGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAg < 4:306019/143‑1 (MQ=37) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTGGACTACGCgg > 3:200064/1‑147 (MQ=255) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑AAAATCGTCCCCAGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgcg > 6:164105/1‑146 (MQ=37) cAGGGCTCACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCGGCAAGGCAGCCCCAAATTGTCGACTACGCGGa < 1:272662/148‑1 (MQ=37) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑T‑CACGCGAACGGCTGCAAGGAAGCCCCAAATTGTCCCCTACCCggc > 7:386885/1‑146 (MQ=37) gTCTCACGAAATTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 1:146588/148‑1 (MQ=255) aCGAAAATAGCAGTAATGGCTGGGGCCGGTTTGCGGCCCTTCGGGGGCAAGCCCGCTCCTACAATGG‑ATATGCGTCCCCTGTTGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt < 2:127149/148‑1 (MQ=38) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGAGGATCAGCGCTc > 7:40074/1‑148 (MQ=37) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGAATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTTTCGCCTACGCGGATCAGCGAt > 1:127149/1‑148 (MQ=37) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa < 3:355507/139‑1 (MQ=255) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGa > 4:355507/1‑139 (MQ=255) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc > 1:71442/1‑148 (MQ=255) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGG‑ATATGCGTACCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGACGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGCTCAGCGATc > 8:305843/1‑148 (MQ=38) gCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAggg < 7:134012/148‑1 (MQ=255) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGCCTACGCGGATCAGCGATCCGGCAGGGTGAt > 7:320899/1‑148 (MQ=255) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATAAGGGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAAATGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt > 2:767/1‑148 (MQ=38) acggCTGGGCCCGCTTTGGGGCCGTGGGGGGGAAAGCCCGCTCCTACAAAGT‑TTATGCGTCCCCTGTTGGTGGGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt < 8:40074/145‑1 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt > 2:243567/1‑147 (MQ=255) cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg > 2:15178/1‑148 (MQ=255) gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATTTGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 6:77053/147‑1 (MQ=255) ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAAG‑ATATGCGTCCCCTGTAGGAGCGGGCTTT‑C‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACCCGGAACACCGATCCGGAAGCGTGATTTTCAGCTc > 8:287991/1‑145 (MQ=25) gCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 1:333981/144‑1 (MQ=255) gCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc > 2:333981/1‑144 (MQ=255) gCCCTGCGCGGCCAACCCCGCTCAAAAAAAGT‑ATATGCGCCCCCTTTTGCAGGGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 4:200064/147‑1 (MQ=255) cccTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcagc > 7:23893/1‑148 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGccc > 5:137376/1‑147 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt < 1:15178/148‑1 (MQ=255) cgGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTg < 8:23893/148‑1 (MQ=255) gggCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTgg < 1:243567/148‑1 (MQ=255) aGCCCGATCCTACAAAGG‑TTATGCGTCCACTGTATGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 7:287991/148‑1 (MQ=39) cGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc < 7:215507/148‑1 (MQ=255) cGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGc < 7:209875/148‑1 (MQ=255) gCGGCCCATGTAGGCGGGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATTCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 8:386885/147‑1 (MQ=255) cccTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGcc < 1:767/148‑1 (MQ=255) ccTGTAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCg < 2:71442/148‑1 (MQ=255) tAGGAGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATg > 5:341670/1‑147 (MQ=255) aGCGGGC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca < 5:164105/147‑1 (MQ=255) gC‑TT‑GCC‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat > 5:77305/1‑147 (MQ=255) | CGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGG‑ATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑C‑CGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAT > NC_002947/1932497‑1932779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |