Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,751	+G	intergenic (+113/+27)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,750	1	.	G	100.0%	250.4 / NA	70	intergenic (+112/+28)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (40/30);  total (40/30)

GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG  >  NC_002947/5674603‑5674889                                                                                                                                                     |                                                                                                                                            ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc                                                                                                                                              <  8:126534/148‑1 (MQ=255) ggTCAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTACTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGCAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc                                                                                                                                              <  1:210974/148‑1 (MQ=255)  gTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCGGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑g                                                                                                                                            >  7:232399/1‑148 (MQ=255)    gAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggc                                                                                                                                          >  2:113484/1‑146 (MQ=255)    gAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑gg                                                                                                                                           <  4:243559/147‑2 (MQ=255)     aGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggc                                                                                                                                          <  2:112225/147‑3 (MQ=255)           cAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCg                                                                                                                                    >  7:106541/1‑148 (MQ=255)             gcgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCGGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGt                                                                                                                                  <  8:232399/148‑1 (MQ=255)               gctgctGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGttt                                                                                                                                <  1:113484/148‑1 (MQ=255)                  gctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATCGGGCCGCAACGCGGCCCCCGCGGGTTTcc                                                                                                                             >  2:312238/1‑147 (MQ=37)                    tgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCGCTCGGGTTTCAg                                                                                                                            >  3:26213/1‑147 (MQ=37)                    tgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCGCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                            >  3:320777/1‑147 (MQ=255)                     gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGc                                                                                                                           <  4:413327/147‑1 (MQ=255)                     gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGAGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                          >  8:311965/1‑148 (MQ=255)                       cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                        >  1:232607/1‑148 (MQ=255)                           cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCgg                                                                                                                                   >  4:253213/1‑133 (MQ=255)                           cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCgg                                                                                                                                   <  3:253213/133‑1 (MQ=255)                               ggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                 >  1:364339/1‑147 (MQ=255)                                    aTAGAAGTACGGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACt                                                                                                             <  1:336148/146‑1 (MQ=255)                                    aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                           >  1:193044/1‑148 (MQ=255)                                     tAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGCTCTCTACAGGAGCGGCCTTGTGTGGTGATAGGGCCGCAAAGCGGCCCCCTCGGGTTTCCGCTGGCAGAAATTACTTCt                                                                                                          >  7:297434/1‑148 (MQ=37)                                          gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGGTTTCCGCTGGCAGAAATTACTTCTGCtt                                                                                                      >  5:269768/1‑147 (MQ=37)                                             ccGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTcaca                                                                                                  <  7:311965/148‑1 (MQ=255)                                                aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTTACACCt                                                                                               <  1:20721/148‑1 (MQ=255)                                                  ggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                             <  2:207245/148‑1 (MQ=255)                                                     gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCGGAAATTACTTCTGCTTCACACCTTCg                                                                                            >  4:362131/1‑146 (MQ=255)                                                     gctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGac                                                                                          >  1:280040/1‑148 (MQ=255)                                                     gatgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATATCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCGGAAATTACTTCTGCTTCACACCTTCg                                                                                            >  4:368066/3‑146 (MQ=37)                                                             gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGatat                                                                                  <  7:26784/148‑1 (MQ=255)                                                                     aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                            >  3:361249/1‑146 (MQ=255)                                                                       cACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                        >  2:418687/1‑148 (MQ=255)                                                                           acacAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGc                                                                                                                           <  2:142084/93‑1 (MQ=255)                                                                           acacAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGc                                                                                                                           >  1:142084/1‑93 (MQ=255)                                                                            cacaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTCCTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCt                                                                   <  8:91532/148‑1 (MQ=255)                                                                             acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                            >  5:350238/1‑138 (MQ=255)                                                                             acaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggt                                                                            <  6:350238/138‑1 (MQ=255)                                                                               aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCGGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                 <  3:362131/147‑1 (MQ=255)                                                                               aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCGGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                 <  3:368066/147‑1 (MQ=255)                                                                                aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 >  2:42069/1‑130 (MQ=255)                                                                                aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtc                                                                                 <  1:42069/130‑1 (MQ=255)                                                                                aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                >  2:4460/1‑147 (MQ=255)                                                                                aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGa                                                               >  1:278032/1‑148 (MQ=255)                                                                                      cTCCTGCAGATTATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                          <  6:331883/147‑1 (MQ=39)                                                                                      cTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGc                                                         <  8:106541/148‑1 (MQ=255)                                                                                       tCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCACTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTCCGAAGTAGc                                                         >  4:246830/1‑147 (MQ=39)                                                                                            cacttaaTTTCTTGATCTCTTCAGGTGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                    <  2:193044/142‑1 (MQ=38)                                                                                                  aTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCttt                                             <  2:232607/148‑1 (MQ=255)                                                                                                        tGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                       >  7:239032/1‑148 (MQ=255)                                                                                                          aTCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATg                                         <  3:383839/144‑1 (MQ=255)                                                                                                           tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                      >  7:375455/1‑146 (MQ=255)                                                                                                               tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTc                                 >  5:154593/1‑147 (MQ=255)                                                                                                                aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                    <  7:4092/127‑1 (MQ=255)                                                                                                                aCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                    >  8:4092/1‑127 (MQ=255)                                                                                                                  aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCGTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                              >  2:69599/1‑147 (MQ=255)                                                                                                                   ggAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttca                            <  2:278032/148‑1 (MQ=255)                                                                                                                     aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                          >  7:230675/1‑148 (MQ=255)                                                                                                                       cGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                        <  2:364339/148‑1 (MQ=255)                                                                                                                              gtgtCGTGATAGGTCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                  <  7:184337/147‑1 (MQ=255)                                                                                                                              gtgtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                  >  6:415637/1‑147 (MQ=255)                                                                                                                              gtgtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCg                   <  1:4460/146‑1 (MQ=255)                                                                                                                               tgtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgt                  <  6:154593/146‑1 (MQ=255)                                                                                                                                gtCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt               >  2:287313/1‑148 (MQ=255)                                                                                                                                  cGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttc                       >  4:411291/1‑138 (MQ=255)                                                                                                                                  cGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttc                       <  3:411291/138‑1 (MQ=255)                                                                                                                                  cGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg              >  6:181152/1‑147 (MQ=255)                                                                                                                                     gATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg           >  3:181518/1‑147 (MQ=255)                                                                                                                                     gATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg          >  2:140391/1‑148 (MQ=255)                                                                                                                                         gggCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt      >  7:277757/1‑148 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc         <  7:17941/143‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc         >  8:17941/1‑143 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt      >  6:86974/1‑146 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt       <  2:253859/145‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg     >  8:179218/1‑147 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa    <  1:287313/148‑1 (MQ=255)                                                                                                                                           gCCGCAAAGCGGCCCCCTCGGTTTTCAGCGGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt       >  1:253859/1‑145 (MQ=255)                                                                                                                                             cGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg  >  8:10750/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                            GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG  >  NC_002947/5674603‑5674889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑