BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,509,703	T→C	N120S (AAC→AGC)	serS ←	serine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,509,703	0	T	C	100.0%	498.3 / NA	135	N120S (AAC→AGC)	serS	serine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (67/68); total (67/68)

Only 100 of 135 total aligned reads displayed.

GGACCACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCA  >  NC_002947/4509559‑4509847

   ccACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTgtcgt                                                                                                                                            <  1:293014/148‑1 (MQ=255)

     aCGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTgtcgtc                                                                                                                                           >  6:230064/1‑147 (MQ=255)

      cGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCt                                                                                                                                          <  3:275885/147‑1 (MQ=255)

       gcagcaCAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTc                                                                                                                                        <  7:80275/148‑1 (MQ=255)

             cagcaAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGgc                                                                                                                                  >  7:316720/1‑148 (MQ=255)

             cagcaAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGgc                                                                                                                                  >  1:206504/1‑148 (MQ=255)

                 aaaaCGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGTGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGgcgc                                                                                                                                >  3:30084/1‑146 (MQ=255)

                 aaaaCGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCg                                                                                                                              <  7:235069/148‑1 (MQ=255)

                  aaaCGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCt                                                                                                                                          >  5:381374/1‑135 (MQ=255)

                  aaaCGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCt                                                                                                                                          <  6:381374/135‑1 (MQ=255)

                       ggCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCgg                                                                                                                         >  6:877/1‑147 (MQ=255)

                             ggACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCt                                                                                                                   <  5:230064/147‑1 (MQ=255)

                                  gCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTc                                                                                                             >  7:296928/1‑148 (MQ=255)

                                  gCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTc                                                                                                             >  2:362134/1‑148 (MQ=255)

                                   cTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTc                                                                                                             <  5:396789/147‑1 (MQ=255)

                                           ccGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGtt                                                                                                    <  8:233981/148‑1 (MQ=255)

                                            cGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTg                                                                                                   >  1:292529/1‑148 (MQ=255)

                                            cGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTg                                                                                                   <  7:203359/148‑1 (MQ=255)

                                             gCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTg                                                                                                   <  4:177371/147‑1 (MQ=255)

                                               ttCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTgg                                                                                                  >  3:322293/1‑146 (MQ=255)

                                                tCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGaa                                                                                               >  2:218173/1‑148 (MQ=255)

                                                 cGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGaa                                                                                               >  3:282010/1‑147 (MQ=255)

                                                   aaGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATgg                                                                                            <  8:182871/148‑1 (MQ=255)

                                                   aaGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATgg                                                                                            >  2:109769/1‑148 (MQ=255)

                                                    aGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGt                                                                                           >  2:388661/1‑148 (MQ=255)

                                                    aGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGt                                                                                           >  8:96938/1‑148 (MQ=255)

                                                      tCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTca                                                                                         <  2:292529/148‑1 (MQ=255)

                                                         aGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTcag                                                                                        >  2:383972/1‑146 (MQ=255)

                                                          ggCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTcagca                                                                                      >  4:335205/1‑147 (MQ=255)

                                                          ggCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGAATTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACATCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTcagcag                                                                                     <  2:245790/148‑1 (MQ=255)

                                                            ccACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGa                                                                                   <  2:121041/148‑1 (MQ=255)

                                                             cACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGa                                                                                   <  4:411092/147‑1 (MQ=255)

                                                             cACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGAt                                                                                  <  1:218173/148‑1 (MQ=255)

                                                               ccGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATg                                                                                 <  5:251711/147‑1 (MQ=255)

                                                                cGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTg                                                                                                   >  7:131950/1‑128 (MQ=255)

                                                                cGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTg                                                                                                   <  8:131950/128‑1 (MQ=255)

                                                                 gCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTcagc                                                                                       >  4:358983/1‑139 (MQ=255)

                                                                 gCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTcagc                                                                                       <  3:358983/139‑1 (MQ=255)

                                                                 gCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCa                                                                              >  1:123287/1‑148 (MQ=255)

                                                                  ttGATTTCACCGAGGGGCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACCGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCAt                                                                             <  2:339723/147‑1 (MQ=255)

                                                                   tGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCAt                                                                             >  4:178894/1‑147 (MQ=255)

                                                                     aTTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATcc                                                                           <  7:145450/147‑1 (MQ=255)

                                                                     aTTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATc                                                                            >  1:373378/1‑146 (MQ=255)

                                                                     aTTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATc                                                                            <  2:373378/146‑1 (MQ=255)

                                                                       ttCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGc                                                                        >  2:173046/1‑148 (MQ=255)

                                                                           ccGAGGGCCACATGGTCCTTGATTTCGAAGGCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCgg                                                                    <  1:226085/148‑1 (MQ=255)

                                                                            cGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCgg                                                                    <  3:377629/147‑1 (MQ=255)

                                                                            cGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGc                                                                   <  1:388661/148‑1 (MQ=255)

                                                                            cGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGc                                                                   >  2:262440/1‑148 (MQ=255)

                                                                              aGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGcc                                                                  <  4:129137/147‑1 (MQ=255)

                                                                               ggcgaccaTGGTCCTGGAGTTCTCAGTGGTTCGGCTTCTGCGTGGCCCAACGGGGCTGTTCGACGCTGTCGTCTTGACTGGCTCCGACCGGGAGGCTGTCGTCCGGCAGGTTGGGAATGGTCATGAGGTTGCCATCCTGCTCGGCCt                                                                 <  4:61812/141‑1 (MQ=255)

                                                                                  ccACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAAt                                                             <  8:260622/148‑1 (MQ=255)

                                                                                   cacaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCgg                                                                    >  6:345743/1‑140 (MQ=255)

                                                                                   cacaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCgg                                                                    <  5:345743/140‑1 (MQ=255)

                                                                                   cacaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATg                                                            >  7:363473/1‑148 (MQ=255)

                                                                                    acaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATgg                                                           <  8:316720/148‑1 (MQ=255)

                                                                                    acaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATgg                                                           <  8:363473/148‑1 (MQ=255)

                                                                                    acaTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATgg                                                           >  1:11002/1‑148 (MQ=255)

                                                                                     caTGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATgg                                                           <  3:178894/147‑1 (MQ=255)

                                                                                       tGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGt                                                        <  1:262440/148‑1 (MQ=255)

                                                                                          tCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTcc                                                      <  8:357987/147‑1 (MQ=255)

                                                                                            cTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTgt                                                                                                                                               <  8:164857/56‑1 (MQ=255)

                                                                                            cTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTgt                                                                                                                                               >  7:164857/1‑56 (MQ=255)

                                                                                               gATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGAt                                                                                  <  4:39519/114‑1 (MQ=255)

                                                                                               gATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGAt                                                                                  >  3:39519/1‑114 (MQ=255)

                                                                                               gATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGa                                                               <  2:249327/133‑1 (MQ=255)

                                                                                               gATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCt                                                  >  8:59678/1‑146 (MQ=255)

                                                                                                aTTTCGAAGACGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCt                                                                                                                   <  5:37492/80‑1 (MQ=255)

                                                                                                aTTTCGAAGACGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCt                                                                                                                   >  6:37492/1‑80 (MQ=255)

                                                                                                 tttCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAg                                               <  4:282010/147‑1 (MQ=255)

                                                                                                   tCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGcc                                             >  3:297733/1‑147 (MQ=255)

                                                                                                         tCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTcag                                                                                        >  4:389507/1‑98 (MQ=255)

                                                                                                           gAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAAt                                                                                              >  3:278295/1‑90 (MQ=255)

                                                                                                           gAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAAt                                                                                              <  4:278295/90‑1 (MQ=255)

                                                                                                           gAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCgg                                      >  7:24384/1‑146 (MQ=255)

                                                                                                            aaCGCCTTCGGTGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGc                                     <  4:30084/146‑1 (MQ=255)

                                                                                                            aaCGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGa                                                                                                                             >  6:46888/1‑58 (MQ=255)

                                                                                                            aaCGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGa                                                                                                                             <  5:46888/58‑1 (MQ=255)

                                                                                                            aaCGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAg                                   <  2:35063/148‑1 (MQ=255)

                                                                                                             tcggcTTCGGCTGTCCGCAACGGAGCACTTCGAGGCGGTGGTCTTCACTGGCGCCGACCGGCACGCTGTCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCGATCGAGCTCGTCCTGAATGGTGTCCAGCTCAGCCTTGCCGGCAg                                   <  6:267838/143‑1 (MQ=255)

                                                                                                              cGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGc                                  >  3:206517/1‑147 (MQ=255)

                                                                                                                  ttCGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGtt                             >  2:115008/1‑148 (MQ=255)

                                                                                                                    cGGCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCg                           >  1:123368/1‑148 (MQ=255)

                                                                                                                      gCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGt                          <  5:415326/147‑1 (MQ=255)

                                                                                                                      gCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAAGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCg                           <  5:137109/146‑1 (MQ=255)

                                                                                                                      gCGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAAGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCg                           >  6:137109/1‑146 (MQ=255)

                                                                                                                       cGTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTc                                                       >  2:374734/1‑117 (MQ=255)

                                                                                                                        gTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGc                                                   <  1:357183/120‑1 (MQ=255)

                                                                                                                        gTGCCCCAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTg                        <  4:322293/147‑1 (MQ=255)

                                                                                                                           ccccAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGcc                     <  1:378690/147‑1 (MQ=255)

                                                                                                                           ccccAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGcc                     <  1:383972/147‑1 (MQ=255)

                                                                                                                           ccccAACGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCa                    <  2:416130/148‑1 (MQ=255)

                                                                                                                                aCGGCGCACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATgcg                >  3:63886/1‑147 (MQ=255)

                                                                                                                                    cgcACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGa           <  7:322840/148‑1 (MQ=255)

                                                                                                                                    cgcACTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGa           <  7:96938/148‑1 (MQ=255)

                                                                                                                                    caacattCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGa           <  1:362134/143‑1 (MQ=255)

                                                                                                                                        cTTCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGt        >  3:41245/1‑147 (MQ=255)

                                                                                                                                          tCGACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCa      >  3:167967/1‑147 (MQ=255)

                                                                                                                                            gACGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg     >  5:275310/1‑146 (MQ=255)

                                                                                                                                             aCGCTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCa  <  8:24384/148‑1 (MQ=255)

GGACCACGCAGCACAGCAAAACGGGCACCGGACAGCTTGGCAGCCGCTTCGAAGTCCAGGCCACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCA  >  NC_002947/4509559‑4509847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`