Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,948,140	C→G	A112P (GCC→CCC)	rho ←	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,948,140	0	C	G	100.0%	180.8 / NA	51	A112P (GCC→CCC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (24/27);  total (24/27)

CCGGTCAAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAA  >  NC_002947/5947998‑5948276                                                                                                                                               |                                                                                                                                         ccGGTCAAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAg                                                                                                                                      >  7:47324/1‑147 (MQ=255)   ggTCAAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTaa                                                                                                                                   <  8:75886/148‑1 (MQ=255)     tCAAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAAc                                                                                                                                  <  6:274674/147‑1 (MQ=255)      cAAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACg                                                                                                                                 >  5:255350/1‑147 (MQ=255)        aGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGc                                                                                                                                <  6:255350/146‑1 (MQ=255)        aGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCt                                                                                                                               <  6:79505/147‑1 (MQ=255)           ccTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTccc                                                                                                                            >  5:230844/1‑147 (MQ=255)             tCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCcctt                                                                                                                         >  2:6089/1‑148 (MQ=255)             tCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGAAGGGGGAAGTAACGCTCCcct                                                                                                                          >  5:101038/1‑147 (MQ=255)                gTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCcctt                                                                                                                         <  6:166516/145‑1 (MQ=255)                    aGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCcttccttc                                                                                                                    <  6:230844/146‑1 (MQ=255)                    aGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCgg                                                                                                                  <  1:6089/148‑1 (MQ=255)                     gCCGTTACCGGCCTCCATCTTCAGGCGCTAATTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGt                                                                                                                 >  1:39172/1‑148 (MQ=255)                      ccGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGt                                                                                                                 >  6:19635/1‑147 (MQ=255)                      ccGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTg                                                                                                                >  1:37162/1‑148 (MQ=255)                        gTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTggg                                                                                                              <  2:29370/148‑1 (MQ=255)                         ttACCCGCCTCCATCTTCAGGCGCGTGTTGGCGCACAGCGGCGGCAGCGTTTCGAACAGGTTCTTGTTCTTCGCGTTTTCCGGGCGGGCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTggg                                                                                                              <  4:355366/147‑1 (MQ=255)                              ggCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGAt                                                                                                         >  4:19021/1‑147 (MQ=255)                                 cTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGAACTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCtt                                                                                                      <  5:19635/147‑1 (MQ=255)                                  tCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCGTCCTTCGGTGGGCGGATCTTg                                                                                                     <  3:249764/147‑1 (MQ=255)                                      tCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCcgac                                                                                                >  2:206873/1‑148 (MQ=255)                                           aGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCcgacg                                                                                               <  3:32320/144‑1 (MQ=255)                                           aGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGAt                                                                                             <  3:286499/146‑1 (MQ=255)                                           aGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATgg                                                                                           >  2:28177/1‑148 (MQ=255)                                                 ttGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCg                                                                                      <  5:236454/147‑1 (MQ=255)                                                      cGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGt                                                                                 >  6:163551/1‑147 (MQ=255)                                                            cAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAgg                                                                          <  2:39172/148‑1 (MQ=255)                                                                  cgTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCg                                                                                      <  6:113472/130‑1 (MQ=255)                                                                  cgTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCg                                                                                      >  5:113472/1‑130 (MQ=255)                                                                      aGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcgg                                                                 <  1:206873/147‑1 (MQ=255)                                                                      aGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATCGTGTCGCCGGTACGCAGGTTGAAAagg                                                                 >  4:25060/1‑147 (MQ=255)                                                                         ttttCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGAAGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGa                                                             >  8:96529/1‑148 (MQ=255)                                                                                aCAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggct                                                      >  8:294441/1‑148 (MQ=255)                                                                                          gTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATTGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCg                                                 <  8:47324/143‑1 (MQ=255)                                                                                           ttcttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCgag                                               <  3:19021/144‑1 (MQ=255)                                                                                               tcGCGTTTTCCGGGCGTTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACTCTCCCCTTCCTTCGGGGGGCGCATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAg                                         >  6:84943/1‑146 (MQ=255)                                                                                                cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGa                                        <  7:294441/146‑1 (MQ=255)                                                                                                         cGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTgg                              <  2:37162/147‑1 (MQ=255)                                                                                                         cGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTgg                              >  6:294157/1‑147 (MQ=255)                                                                                                                tCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCa                      <  1:381718/148‑1 (MQ=255)                                                                                                                     gTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAg                 <  1:181303/148‑1 (MQ=255)                                                                                                                          tGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACg             >  1:321370/1‑147 (MQ=255)                                                                                                                                 gACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTACGGTGGGCGGATCATGACGACGATGGTGTCGCCGGTACCCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGTCCGTCCAGGAAGGACGCATCAGc      >  3:176868/1‑147 (MQ=255)                                                                                                                                   ccTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCa                                                                            >  3:372390/1‑75 (MQ=255)                                                                                                                                   ccTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCa                                                                            <  4:372390/75‑1 (MQ=255)                                                                                                                                     ttCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTa                  <  3:146144/131‑1 (MQ=255)                                                                                                                                     ttCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTa                  >  4:146144/1‑131 (MQ=255)                                                                                                                                     ttCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGaa  >  6:86540/1‑147 (MQ=255)                                                                                                                                     ttCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGaa  <  5:294157/147‑1 (MQ=255)                                                                                                                                        agcagGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGggccgg                         <  5:250245/121‑1 (MQ=255)                                                                                                                                        agcagGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGggccgg                         >  6:250245/1‑121 (MQ=255)                                                                                                                                               |                                                                                                                                         CCGGTCAAGTCCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAA  >  NC_002947/5947998‑5948276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑