Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,819 2 bp→CT intergenic (+56/+696) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8190TC98.0% 155.0 / NA 50intergenic (+56/+697)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (25/23);  minor base A (1/0);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0029474,740,8200GT100.0% 164.7 / NA 50intergenic (+57/+696)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/24);  total (26/24)

CCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740678‑4740952
                                                                                                                                             ||                                                                                                                                      
ccAGCCCGTACAAGATCGGCCGTACGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCGGAAAGACCGCAAGTAAGCTGCAGTAGCCGACCGCAAGAAGGCTGCCCTCGAGCCGCC‑TTTGTTATTTGTCATGTGcc                                                                                                                                   >  6:315882/1‑147 (MQ=255)
 cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                 >  7:93281/1‑148 (MQ=255)
  agctagTACATGATCGGCCGTCCGCGCCAGCTGTACACCGTCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCTCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGATGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                 <  5:315882/142‑1 (MQ=255)
       gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCc‑‑tct                                                                                                                          >  6:148389/1‑147 (MQ=255)
          cAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTcgc                                                                                                                      <  8:93281/148‑1 (MQ=255)
           aaGATCGGTCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTcg                                                                                                                       <  6:179056/146‑1 (MQ=255)
           aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTcgc                                                                                                                      <  3:106750/147‑1 (MQ=255)
              aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGcc                                                                                                                  >  1:410518/1‑148 (MQ=255)
                       ccGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCcgcg                                                                                                         <  7:707/148‑1 (MQ=255)
                        cgcgCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCcgcg                                                                                                         <  2:410518/147‑1 (MQ=255)
                          cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTAGCGGCCTTGCCcgc                                                                                                          >  6:171722/1‑144 (MQ=255)
                           gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGGCAAGGCCGCGAag                                                                                                      <  6:25825/147‑1 (MQ=255)
                           gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGGCAAGGCCGCGAag                                                                                                      <  5:148389/147‑1 (MQ=255)
                            ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAagag                                                                                                    >  7:175445/1‑148 (MQ=255)
                                cTGGACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTACGCTGCACTAGCCGCACGCAAGACGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGcc                                                                                                 <  3:84617/147‑1 (MQ=255)
                                 tGTACACGGGCGAGCATACGCGCGACATCGTTGCCCTGAAAGCCCGCAAGTACGCTGCAGTAGCCGACCGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                <  6:348862/147‑1 (MQ=255)
                                   tACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTATCTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCg                                                                                                <  1:306179/146‑1 (MQ=255)
                                    acacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTAGCGGCCTTTCCCGCGAAGAGGCCGGca                                                                                             >  2:132402/1‑147 (MQ=255)
                                       ccGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCATGCCCGCGAAGAGGCCGGccca                                                                                           >  5:141197/1‑146 (MQ=255)
                                                ataaGCGCGACATCGTTGCCCTCAACCCCCGAAATTAAGCTGCTGTAGCCGAACCCAAGAAGGCTGCCCTGGGGCAG‑CTTTTTTTTTTTGTCGTGTGCCGGCC‑‑TCTTCGCGGCGTGGCCCGCTAAGAGGCCGGCACAGACACCACaaa                                                                                <  2:147423/146‑1 (MQ=255)
                                                   aGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCGTGCCCGCGAAGAGGCCGGCCCCGCCACCCCcaaa                                                                               >  3:387471/1‑146 (MQ=255)
                                                      gcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGCACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCCGCC‑‑ACTTCCCGGCCATGCCCGCGAAGACGCCGCCCCAGACACCACAAAAccac                                                                           >  3:48183/1‑145 (MQ=255)
                                                      gcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAAcc                                                                             >  6:209947/1‑145 (MQ=255)
                                                      gcgACATCGTTCCCCTTAAAGCCCGCATGTAAGCTGCATTAGCCGAACGCATGATGGCTGCCCTGGGGATGCGTTTTTTT‑TTTGTCCTGTGCGGGCG‑‑TCTTCGCGGCGTTGCCCGGTATTAGGCCGGCACAGACACCACAAAACCTc                                                                           <  6:141197/147‑1 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                <  4:171337/91‑1 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                >  3:171337/1‑91 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTAGCGGCCTTGCCCGCGAAGAGGGCGGCACAGACACCACAAAACCTCAGttt                                                                      >  4:223621/1‑147 (MQ=255)
                                                                    cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCATAGCGGCCGTGCCCGCGCAGCGGGCGGCACAGACACCCCAAAACCACAGTTTTTGTacgccc                                                            >  1:415437/1‑148 (MQ=255)
                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                           >  7:206380/1‑148 (MQ=255)
                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGAGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                           >  1:106788/1‑148 (MQ=255)
                                                                        aaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTc                                                        >  7:272067/1‑148 (MQ=255)
                                                                            ccGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCCTTATTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCAc                                                      <  1:132402/148‑1 (MQ=255)
                                                                             cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCGTGCCCGCGGAGAGGCCGGCACCGACACCACAAAAACTCAGTTTTTCTCCGCCCGCGCACGc                                                    >  4:235083/1‑147 (MQ=255)
                                                                                 aGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TATTCGCGGCCTTGCCCGCCAAGAGGCCGGCACAGACACCACAAAACCACAGTTTTTCTCCGCCCGCTCACGCAccc                                                >  2:348235/1‑147 (MQ=255)
                                                                                     aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGCCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                           <  1:139585/148‑1 (MQ=255)
                                                                                       cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                         <  7:81128/148‑1 (MQ=255)
                                                                                       cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                         <  7:43206/148‑1 (MQ=255)
                                                                                       cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑ACATAGCGGCCTTGCCCGCGAAGACGCCGGCACAGACACCACAAAACCACAGGTTTTATCCGCCCCCTCACGCACCCCattaa                                          >  3:149676/1‑145 (MQ=255)
                                                                                        tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAg                                         <  5:209947/147‑1 (MQ=255)
                                                                                           aGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGc                                                                                                                   <  2:418417/70‑1 (MQ=37)
                                                                                           aGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGc                                                                                                                   >  1:418417/1‑70 (MQ=37)
                                                                                                 cGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TATTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCCACCACAAACCCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGaa                                >  6:95901/1‑147 (MQ=255)
                                                                                                  gAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTAGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGCCACCACAAAACCTGCGTTTTTCTCCGCCCGCGAACGCAGCCCTTTCAGGGTTTTGAACg                              >  2:57146/1‑148 (MQ=255)
                                                                                                   aaCGCACGAAGGCTGCCCGCGGGCAGCC‑TTTTTTATTTCTCCTGTGCCGGCC‑‑GCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACgg                             <  8:110519/148‑1 (MQ=255)
                                                                                                     cGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                            >  6:195357/1‑147 (MQ=255)
                                                                                                         agaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCCGCCGTGCCCGCGAAGCGGCCGGCACAGACCCCCCAAAACCACCGGTTTTCTCCGCCCGCTCACGCAGCCCTTTGAGGGTATTTAACGGCGCAt                        >  5:361903/1‑147 (MQ=255)
                                                                                                             ggCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                   <  8:272067/148‑1 (MQ=255)
                                                                                                                        ggCAGCC‑TTTTTTATTTGTCCTGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTGACGCAGCCCTTTCAGGGTATTGAACGGCGCCTCCACCCCGAACttgt         >  5:92585/1‑147 (MQ=255)
                                                                                                                               cttttttttttGTCCTGTGCGGGCC‑‑GCTTCGCGGCGTTGCGCGCTATGAGGCGGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGcca   <  4:48183/146‑1 (MQ=255)
                                                                                                                                 tttttATTTGTCCTGTGCCGGCC‑‑TCTGCGCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccac  <  8:206380/146‑1 (MQ=255)
                                                                                                                                             ||                                                                                                                                      
CCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCC‑‑TCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCAC  >  NC_002947/4740678‑4740952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑