Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	612,730	Δ1 bp	6.8%	coding (1452/1896 nt)	dxs ←	1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	612,730	0	A	.	6.8%	270.6 / 13.0	88	coding (1452/1896 nt)	dxs	1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (37/44);  new base . (3/3);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGATCTGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGC  >  NC_002947/612582‑612878                                                                                                                                                      |                                                                                                                                                      cGTTGATCTGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCa                                                                                                                                                       <  5:42610/149‑1 (MQ=255) cGTTGATCTGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCa                                                                                                                                                       >  7:287688/1‑149 (MQ=255)     gATCTGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAAGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCAcgcg                                                                                                                                                   >  6:101405/1‑149 (MQ=255)       tCTGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCg‑gg                                                                                                                                                >  5:143109/1‑149 (MQ=255)         tGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCAcgcgagat                                                                                                                                               >  7:129770/1‑145 (MQ=255)         tGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCAcgc                                                                                                                                                    <  8:129770/144‑1 (MQ=255)              ggCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGt                                                                                                                                          <  8:102906/148‑1 (MQ=255)              ggCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTa                                                                                                                                         >  2:49841/1‑149 (MQ=255)                    ttGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCgg                                                                                                                                   >  1:134910/1‑149 (MQ=255)                       cATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCggcc                                                                                                                                 <  3:56856/148‑1 (MQ=255)                        aTGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCggccgg                                                                                                                               <  6:362410/149‑1 (MQ=255)                        aTGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCggccgg                                                                                                                               <  5:186129/149‑1 (MQ=255)                          ggCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCggccgggc                                                                                                                             >  8:308997/1‑149 (MQ=255)                           gCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCggccgggcc                                                                                                                            <  2:374901/149‑1 (MQ=255)                            ccTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCggccgggccg                                                                                                                           <  4:249025/149‑1 (MQ=255)                             cTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACC‑CGCGTGGTACCggc                                                                                                                                         <  6:194706/134‑3 (MQ=255)                             cTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACC‑CGCGTGGTACCggc                                                                                                                                         >  5:194706/1‑132 (MQ=255)                                ggCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGtt                                                                                                                         <  8:287688/147‑1 (MQ=255)                                ggCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGtt                                                                                                                         <  4:336425/147‑1 (MQ=255)                                ggCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGtt                                                                                                                         <  8:85417/147‑1 (MQ=255)                                ggCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTg                                                                                                                        <  1:322351/148‑1 (MQ=255)                                ggCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGGGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGtt                                                                                                                         <  5:1396/147‑1 (MQ=255)                                  ccAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCTCGATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAACGGCCCGGCCGCTGTa                                                                                                                      <  1:304945/149‑1 (MQ=255)                                      ttGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGt                                                                                                                 <  1:259888/149‑1 (MQ=255)                                          aCGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGcc                                                                                                             >  2:32717/1‑149 (MQ=255)                                            gCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCgg                                                                                                           >  7:312375/1‑149 (MQ=255)                                              cGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTg                                                                                                         <  6:143109/149‑1 (MQ=255)                                               gAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGc                                                                                                        <  2:329324/149‑1 (MQ=255)                                                  cacCAGCAGGTCCACTTTTTCGCCCTGGCGGCGGACCAGGCCCTTGCCGATCTCCAGTGGTTGCAGTGCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTTCTGa                                                                                                     <  7:363050/149‑1 (MQ=255)                                                  cacCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGa                                                                                                     <  1:49841/149‑1 (MQ=255)                                                  cacCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGATACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGa                                                                                                     <  4:3119/149‑1 (MQ=255)                                                  cacCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAAGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GTTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGa                                                                                                     <  5:101405/149‑1 (MQ=255)                                                   acCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAg                                                                                                    >  7:49423/1‑149 (MQ=255)                                                     cagcagGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTg                                                                                                         <  2:333842/142‑1 (MQ=255)                                                     cagcagGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTg                                                                                                         >  1:333842/1‑142 (MQ=255)                                                       gcagGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATc                                                                                                >  5:310476/1‑149 (MQ=255)                                                             ccACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTgcg                                                                                           >  7:313678/1‑148 (MQ=255)                                                             ccACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAACggcccggccgc                                                                                                                          >  5:101381/1‑116 (MQ=255)                                                             ccACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAACggcccggccgc                                                                                                                          <  6:101381/117‑2 (MQ=255)                                                              cACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGa                                                                                                     <  2:141772/137‑1 (MQ=255)                                                              cACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGa                                                                                                     >  1:141772/1‑137 (MQ=255)                                                                cTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAgt                                                                                       <  2:378257/149‑1 (MQ=255)                                                                 tttttCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAgtt                                                                                      >  8:7344/1‑149 (MQ=255)                                                                         ccTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCtcg                                                                              >  8:167304/1‑149 (MQ=255)                                                                         ccTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACC‑CGCGTGGTACCgg                                                                                                                                          <  6:14433/89‑2 (MQ=255)                                                                         ccTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACC‑CGCGTGGTACCgg                                                                                                                                          >  5:14433/1‑88 (MQ=255)                                                                          cTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCtcgt                                                                             >  2:97986/1‑149 (MQ=255)                                                                                gcggACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGTGCATCTTGCGCAGTTCGTTCTCGTCGCTgg                                                                       <  1:32717/149‑1 (MQ=255)                                                                                gcggACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTgg                                                                       <  6:310476/149‑1 (MQ=255)                                                                                gcggACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTgg                                                                       >  2:382340/1‑149 (MQ=255)                                                                                 ctgACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTggg                                                                      <  4:372016/147‑1 (MQ=255)                                                                                    aCCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGt                                                                   <  1:97986/149‑1 (MQ=255)                                                                                    aCCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGt                                                                   <  1:382340/149‑1 (MQ=255)                                                                                     ccACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGtc                                                                  <  8:49423/149‑1 (MQ=255)                                                                                      cACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTAc                                                                                                                     <  2:121747/97‑1 (MQ=255)                                                                                      cACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCA‑GGTACCGTACAGCGGCCGGGCCGTTGTAc                                                                                                                     >  1:121747/1‑97 (MQ=255)                                                                                      cACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTACGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGCTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGtca                                                                 >  8:263475/1‑149 (MQ=255)                                                                                            cTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCa                                                           <  4:80835/149‑1 (MQ=255)                                                                                             ttGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAg                                                          >  5:42249/1‑149 (MQ=255)                                                                                                       ccAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCgg                                                 >  3:179533/1‑148 (MQ=255)                                                                                                       ccAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCgg                                                 >  1:158692/1‑148 (MQ=255)                                                                                                        cAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCggg                                                <  2:134910/148‑1 (MQ=255)                                                                                                        cAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCgg                                                 <  7:308997/147‑1 (MQ=255)                                                                                                         aGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGAt                                              <  8:312375/149‑1 (MQ=255)                                                                                                             gTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAg                                          >  5:75352/1‑149 (MQ=255)                                                                                                              ttGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAgc                                         >  4:138926/1‑149 (MQ=255)                                                                                                                 cAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAgcg                                        >  7:338983/1‑147 (MQ=255)                                                                                                                 cAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCa                                      <  6:42249/149‑1 (MQ=255)                                                                                                                 cAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCa                                      <  7:167304/149‑1 (MQ=255)                                                                                                                          gCT‑GATCGGCTCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGAGCAGTTCGTTCTCGTCGCTGGTGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACa                             <  5:4152/149‑1 (MQ=255)                                                                                                                          gCT‑GATCGGCGCATTCGGGCCGGTACCCCGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGa                               >  3:42530/1‑147 (MQ=255)                                                                                                                          gCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGa                               >  6:327166/1‑147 (MQ=255)                                                                                                                                tCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAgcgc                                       >  2:346094/1‑134 (MQ=255)                                                                                                                                tCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAgcgc                                       <  1:346094/134‑1 (MQ=255)                                                                                                                                  ggCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTa                      <  1:326467/149‑1 (MQ=255)                                                                                                                                  ggCGCATTCGGGCCGGTACCACACG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCg                        >  2:169770/1‑147 (MQ=255)                                                                                                                                   gcgcATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCGGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAg                     >  3:111697/1‑149 (MQ=255)                                                                                                                                   gcgcATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAg                     <  4:179533/149‑1 (MQ=255)                                                                                                                                       aTTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGc                 >  4:324518/1‑149 (MQ=255)                                                                                                                                          ctgtacGGTACC‑CGCGTGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAgcg                                        <  6:174034/118‑1 (MQ=255)                                                                                                                                          ctgtacGGTACC‑CGCGTGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCAGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAgcg                                        >  5:174034/6‑123 (MQ=255)                                                                                                                                            ggCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCg                        >  7:225633/1‑137 (MQ=255)                                                                                                                                            ggCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCg                        <  8:225633/137‑1 (MQ=255)                                                                                                                                            ggCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCg            >  7:236209/1‑149 (MQ=255)                                                                                                                                               cGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTgg         <  3:163996/149‑1 (MQ=255)                                                                                                                                                 gTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGt       >  5:161308/1‑149 (MQ=255)                                                                                                                                                 gTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGAGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGt       >  2:208525/1‑149 (MQ=255)                                                                                                                                                    ccACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCg                                                  >  3:159368/1‑103 (MQ=255)                                                                                                                                                    ccACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCg                                                  <  4:159368/103‑1 (MQ=255)                                                                                                                                                     cACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCggg   >  3:334708/1‑149 (MQ=255)                                                                                                                                                      aCGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGc  >  4:291494/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                      CGTTGATCTGTTCGGCCACTTGCATGGCCTCGGCCAGTTGTACGCCGAACACCAGCAGGGCCACTTTTTCGCCCTGGCGGCGGACCACGCCCTTGCCGATCTCCAGTGGTTGCAGGTCTCCGCT‑GATCGGCGCATTCGGGCCGGTACCACGCG‑GGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGC  >  NC_002947/612582‑612878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑