BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,300,027	Δ1 bp	6.4%	coding (151/1608 nt)	PP_1134 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,300,027	0	C	.	6.4%	402.6 / 19.8	125	coding (151/1608 nt)	PP_1134	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (56/61); new base . (4/4); total (60/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 125 total aligned reads displayed.

GCTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTCGTA  >  NC_002947/1299883‑1300173

gcTCCTGCAGGCCTGGCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑Tg                                                                                                                                                <  2:201327/149‑1 (MQ=255)

gcTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑Tg                                                                                                                                                <  2:360130/149‑1 (MQ=255)

  tCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCTGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGtt                                                                                                                                              >  8:231508/1‑149 (MQ=255)

  tCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGtt                                                                                                                                              >  1:103619/1‑149 (MQ=255)

     tGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGttct                                                                                                                                            >  7:351718/1‑148 (MQ=255)

       cAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGCGAGCT‑GC‑TGttcat                                                                                                                                           <  6:391407/147‑3 (MQ=255)

       cAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGttcttc                                                                                                                                          <  6:191152/148‑1 (MQ=255)

        aGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTcttctt                                                                                                                                        <  2:103619/149‑1 (MQ=255)

            cTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTgcgc                                                                                                                                    <  1:171074/149‑1 (MQ=255)

            cTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTgcgc                                                                                                                                    >  4:367700/1‑149 (MQ=255)

            cTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTgcgc                                                                                                                                    >  3:361397/1‑149 (MQ=255)

             tGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTgcgcg                                                                                                                                   >  1:49885/1‑149 (MQ=255)

             tGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTgcgcg                                                                                                                                   >  8:112455/1‑149 (MQ=255)

               ccGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCa                                                                                                                                 >  3:78747/1‑149 (MQ=255)

                cGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCAt                                                                                                                                >  2:53547/1‑149 (MQ=255)

                     ccTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAg                                                                                                                           <  1:149117/149‑1 (MQ=255)

                        caccGACGAACTTGTAGGTGACGTCTGGCGCCGGGCCGAAGTAGCGATCATCGGCGAACATGCTGAGCAGCTCTGCCTCGTTGCTGCCTTCCAGATCGATAGTGTAGATCAACACC‑AGCTCGC‑TGTTCTTCATGCGCGCAACGTAGCTg                                                                                                                        >  8:289243/3‑149 (MQ=255)

                            gacgaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCt                                                                                                                    <  2:370618/149‑1 (MQ=255)

                             acgaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCtt                                                                                                                   <  4:361397/149‑1 (MQ=255)

                               gaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTgcgc                                                                                                                     <  2:64464/145‑1 (MQ=255)

                               gaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTg                                                                                                                  >  4:142768/1‑148 (MQ=255)

                                   ttGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCTGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAg                                                                                                             >  3:144675/1‑149 (MQ=255)

                                   ttGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAg                                                                                                             >  7:229369/1‑149 (MQ=255)

                                     gTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAgg                                                                                                            <  4:229129/148‑1 (MQ=255)

                                      tAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGtt                                                                                                          <  8:229369/149‑1 (MQ=255)

                                      tAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGt                                                                                                           <  5:284272/148‑1 (MQ=255)

                                              gTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGTTGTAGATGAACAGC‑AGCTCTC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTTTAGCTGa                                                                                                  <  8:188424/149‑1 (MQ=255)

                                              gTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCTCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGa                                                                                                  <  1:53547/149‑1 (MQ=255)

                                              gTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGa                                                                                                  <  8:351718/149‑1 (MQ=255)

                                               tCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTCGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAg                                                                                                 >  2:83898/1‑149 (MQ=255)

                                                   gggccgggccGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATTAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCa                                                                                               <  6:369666/147‑1 (MQ=255)

                                                      ccgggccgATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGc                                                                                          <  3:142768/149‑1 (MQ=255)

                                                       cgggccgATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCt                                                                                         >  7:303197/1‑149 (MQ=255)

                                                           ccgATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCtcgtc                                                                                     >  2:380358/1‑149 (MQ=255)

                                                             gATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGGTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTcgc                                                                                   <  8:282840/149‑1 (MQ=255)

                                                             gATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTcgc                                                                                   <  5:324968/149‑1 (MQ=255)

                                                                  tGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑t                                                                                                                                                 >  8:170354/1‑82 (MQ=255)

                                                                     gATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTg                                                                                                         >  4:773/1‑119 (MQ=255)

                                                                     gATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGt                                                                                             <  6:388312/131‑1 (MQ=255)

                                                                     gATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGt                                                                                             >  5:388312/1‑131 (MQ=255)

                                                                     gATCATCGGCGAACTTGCTGAGAAGCTCGTCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGccc                                                                           <  7:268133/149‑1 (MQ=255)

                                                                       tcatcGGAGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAg                                                                         <  3:209930/149‑1 (MQ=255)

                                                                         ctcGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAgg                                                                        <  4:220100/147‑1 (MQ=255)

                                                                         atcGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAgg                                                                        <  4:333427/148‑1 (MQ=255)

                                                                          tcGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGgcg                                                                      >  6:240099/1‑149 (MQ=255)

                                                                            ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGgcgc                                                                     <  7:112455/148‑1 (MQ=255)

                                                                            ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCt                                                                    <  6:195580/149‑1 (MQ=255)

                                                                            ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCt                                                                    >  1:45142/1‑149 (MQ=255)

                                                                            ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCt                                                                    <  3:372095/149‑1 (MQ=255)

                                                                             gCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGgc                                                                       >  7:103738/1‑145 (MQ=255)

                                                                              cGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTgg                                                                  <  7:289243/149‑1 (MQ=255)

                                                                                     gCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGAt                                                             <  4:78747/147‑1 (MQ=255)

                                                                                      cTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATgg                                                           <  2:45142/148‑1 (MQ=255)

                                                                                      cTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGc                                                          <  4:114041/149‑1 (MQ=255)

                                                                                       tGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCt                                                         >  4:244739/1‑149 (MQ=255)

                                                                                         agcagcTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTc                                                       >  6:256676/1‑149 (MQ=255)

                                                                                            agcTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTcgcg                                                    <  3:158918/149‑1 (MQ=255)

                                                                                             gcTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTcgcg                                                    >  8:189040/1‑148 (MQ=255)

                                                                                              cTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCa                                                  <  6:365258/149‑1 (MQ=255)

                                                                                                 ggCGTCGTTGCTGGCTTCCAGATCGAAGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGGAg                                                 <  4:144675/147‑1 (MQ=255)

                                                                                                 ggCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAg                                                 <  7:189040/147‑1 (MQ=255)

                                                                                                        ttGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgtc                                        >  7:179293/1‑149 (MQ=255)

                                                                                                         agCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCAGCGCTTGAACAGCTTGAATCTGAGAAGTTGCTCGTCGCGGATGCCCAGGCGCTTGACGATGGCTTCGCGCAGCGCTtcgtcg                                       >  5:390111/2‑149 (MQ=255)

                                                                                                          gCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgtcg                                       <  5:256676/148‑1 (MQ=255)

                                                                                                              gCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATg                                  <  7:293160/149‑1 (MQ=255)

                                                                                                              gCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATg                                  >  3:332266/1‑149 (MQ=255)

                                                                                                                ttCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGt                                >  4:96204/1‑149 (MQ=255)

                                                                                                                ttCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGt                                >  4:161818/1‑149 (MQ=255)

                                                                                                                  ccAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTc                               <  3:367700/148‑1 (MQ=255)

                                                                                                                    aGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATgg                                                           >  6:146261/1‑118 (MQ=255)

                                                                                                                    aGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATgg                                                           <  5:146261/118‑1 (MQ=255)

                                                                                                                    aGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgtcg                                       <  2:186487/138‑1 (MQ=255)

                                                                                                                     gATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGccc                                                                           >  3:260304/1‑101 (MQ=255)

                                                                                                                     gATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGccc                                                                           <  4:260304/101‑1 (MQ=255)

                                                                                                                     gATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTc                               <  8:350330/145‑1 (MQ=255)

                                                                                                                     gATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTc                               >  7:350330/1‑145 (MQ=255)

                                                                                                                     gATCGATGGTGTAGATGAACAGC‑AGCACGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTTAAGCTGAGCAGTGCCTCGTCGCGGAAGCCCAGGCGCTCGACGAAGGCTTCGCGCAGCGATTCGTCGGCATGGACCAgg                           >  4:224336/1‑149 (MQ=255)

                                                                                                                      aTCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCgg                                      <  1:171929/137‑1 (MQ=255)

                                                                                                                      aTCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCgg                                      >  2:171929/1‑137 (MQ=255)

                                                                                                                         gATGGTGTAGATGAACAGC‑AGCTCGCGTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAgggg                         >  1:3834/1‑148 (MQ=255)

                                                                                                                         gATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAgggg                         >  5:212084/1‑147 (MQ=255)

                                                                                                                         gATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAgggg                         >  3:259195/1‑147 (MQ=255)

                                                                                                                         gATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGc                        <  8:303197/148‑1 (MQ=255)

                                                                                                                         gATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCa                       <  4:332266/149‑1 (MQ=255)

                                                                                                                          aTGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAg                      >  6:166598/1‑149 (MQ=255)

                                                                                                                             gtgtAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGACCAGGGGCAGCt                    >  8:43412/1‑148 (MQ=255)

                                                                                                                                aagaagaACAGCGAGCT‑GC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGAt                                                             <  3:299808/103‑1 (MQ=255)

                                                                                                                                aagaagaACAGCGAGCT‑GC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGAt                                                             >  4:299808/2‑104 (MQ=255)

                                                                                                                                aagaagaACAGCGAGCT‑GC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAg                >  8:275567/2‑149 (MQ=255)

                                                                                                                                aagaagaACAGCGAGCT‑GC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAg                >  5:400375/2‑149 (MQ=255)

                                                                                                                                  gaagAACAGCGAGCT‑GC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTGCGCGCAGCGCTTCGTCGGGATGGT‑CTGGGGCAGCTTCAGTTc             >  8:364140/4‑149 (MQ=255)

                                                                                                                                      aaCAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGGGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgt                                         >  7:67601/1‑118 (MQ=255)

                                                                                                                                      aaCAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCgg           <  8:179293/148‑1 (MQ=255)

                                                                                                                                      aaCAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGt          <  3:247358/149‑1 (MQ=255)

                                                                                                                                      aaCAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGt          <  3:161818/149‑1 (MQ=255)

                                                                                                                                         agc‑agcTCAC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGAt       >  5:77794/1‑149 (MQ=255)

                                                                                                                                           c‑agcTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTc     >  7:68975/1‑149 (MQ=255)

                                                                                                                                              gcTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTCg    >  8:130125/1‑148 (MQ=255)

                                                                                                                                               cTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTCGTa  <  4:259195/149‑1 (MQ=255)

                                                                                                                                               cTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTCGTa  <  7:179419/149‑1 (MQ=255)

GCTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGC‑TGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTCGTA  >  NC_002947/1299883‑1300173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`