Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,498,898 Δ1 bp 5.7% coding (799/2454 nt) PP_2193 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,498,8980G. 5.7% 227.9 / 6.5 70coding (799/2454 nt)PP_2193ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (31/35);  new base . (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01

GAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAGCAGAACGAAG  >  NC_002947/2498750‑2499042
                                                                                                                                                       |                                                                                                                                                
gAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTg                                                                                                                                                  >  2:333833/1‑149 (MQ=255)
 aaCAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTgg                                                                                                                                                 >  7:276015/1‑149 (MQ=255)
      cTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCgg                                                                                                                                            >  1:134714/1‑149 (MQ=255)
      cTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCgg                                                                                                                                            <  2:248268/149‑1 (MQ=255)
         ccGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGgcgc                                                                                                                                         >  6:386188/1‑149 (MQ=255)
             ggCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCtt                                                                                                                                     <  6:309337/149‑1 (MQ=255)
              gCCTGTGGCGCCAGTTCGACAGCTTTTCGCCGTTCAACGTCGAGCGGGGCTATGTGCTGTGTGGCGCCAGCGC‑AA‑‑TCTCCGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTc                                                                                                                                    <  6:236139/149‑1 (MQ=255)
               ccTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCg                                                                                                                                   <  3:329989/149‑1 (MQ=255)
                cTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCACCGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGGGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGa                                                                                                                                  <  3:243122/149‑1 (MQ=255)
                   gcgcgCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTCCGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGaaa                                                                                                                                <  5:195797/147‑1 (MQ=255)
                           gTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTgg                                                                                                                       >  7:160627/1‑149 (MQ=255)
                              cGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTgggg                                                                                                                     <  8:276015/148‑1 (MQ=255)
                               gACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGc                                                                                                                   >  3:100710/1‑149 (MQ=255)
                                aCAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGCACAGTTCCATACCCCCAGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTgggg                                                                                                                     <  1:324378/149‑1 (MQ=255)
                                  aGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCACGGCGCGCTTCGAAACCCAGTTGGGGGc                                                                                                                   >  5:258046/1‑146 (MQ=255)
                                    cATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCcag                                                                                                                >  4:131222/1‑147 (MQ=255)
                                      tttCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCagcagc                                                                                                            >  5:28721/1‑149 (MQ=255)
                                            cGTTCAACGTCGTGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTc                                                                                                      <  2:108131/149‑1 (MQ=255)
                                                        aGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTACAACAACAGcgac                                                                                          <  3:135909/149‑1 (MQ=255)
                                                              tCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTg                                                                                    >  6:354274/1‑149 (MQ=255)
                                                                     gCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCAccc                                                                             >  4:91336/1‑149 (MQ=255)
                                                                      gccggtGGCGCCACCGC‑CG‑‑TAGATGGCGCTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCg                                                                            >  1:125410/6‑149 (MQ=38)
                                                                           tGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCg                                                                       >  7:367006/1‑149 (MQ=255)
                                                                             gcgccagcgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTc                                                                        <  5:386188/146‑1 (MQ=255)
                                                                             gcgccagcgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGAAGACCTGGCCACCCCCTTCGcc                                                                     >  4:348032/1‑149 (MQ=255)
                                                                               gccagcgc‑ca‑‑TCTCCGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCa                                                                   <  8:160627/149‑1 (MQ=255)
                                                                                ccagcgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAg                                                                  >  2:326960/1‑149 (MQ=255)
                                                                                 cagcgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATGATCATCATCGTGACAAAGAAGGGCGAGCCTGGCGCGGCGCGCTTCGAAACGCAGTTGGGGGCCATCAGCGGTTTCAACAACAGCGACGACCTGGACACCCGCTTCGCCCAGt                                                                 <  6:367511/149‑1 (MQ=255)
                                                                                 cagcgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGt                                                                 >  2:351560/1‑149 (MQ=255)
                                                                                 cagcgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGt                                                                 >  2:356140/1‑149 (MQ=255)
                                                                                    cgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAacaaca                                                                                               >  6:231264/1‑116 (MQ=255)
                                                                                    cgc‑ca‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAacaaca                                                                                               <  5:231264/116‑1 (MQ=255)
                                                                                                cggcggTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCT‑GCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCg                                                        >  8:259692/1‑145 (MQ=255)
                                                                                                cggcggTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCT‑GCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCg                                                        <  1:373632/145‑1 (MQ=255)
                                                                                                cggcggTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCT‑GCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCg                                                        <  7:259692/145‑1 (MQ=255)
                                                                                                cggcggTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCT‑GCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCg                                                        >  2:373632/1‑145 (MQ=255)
                                                                                                 ggcggTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCggtgg                                                    <  1:58554/149‑1 (MQ=255)
                                                                                                        gcgcCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGa                                              <  3:340945/148‑1 (MQ=255)
                                                                                                        gcgcCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGa                                              <  1:9439/148‑1 (MQ=255)
                                                                                                          gcCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCggtggt                                                   >  8:319057/1‑141 (MQ=255)
                                                                                                          gcCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCggtggt                                                   <  7:319057/141‑1 (MQ=255)
                                                                                                          gcCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCg                                           >  4:111643/1‑149 (MQ=255)
                                                                                                           ccACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCgg                                          <  6:28721/149‑1 (MQ=255)
                                                                                                            cACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCggg                                         >  5:201012/1‑149 (MQ=255)
                                                                                                             aCCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGt                                        <  3:91336/149‑1 (MQ=255)
                                                                                                             aCCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGt                                        <  1:326960/149‑1 (MQ=255)
                                                                                                                 gcggcATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAAt                                    >  6:125283/1‑149 (MQ=255)
                                                                                                                       tcatcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCgg                              >  2:353055/1‑149 (MQ=255)
                                                                                                                         atcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGtt                            <  1:356140/149‑1 (MQ=255)
                                                                                                                         atcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGtt                            <  1:351560/149‑1 (MQ=255)
                                                                                                                          tcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAAt                                    >  5:187297/1‑140 (MQ=255)
                                                                                                                          tcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAAt                                    <  6:187297/140‑1 (MQ=255)
                                                                                                                          tcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGccc                                >  3:365766/1‑144 (MQ=255)
                                                                                                                          tcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGccc                                <  4:365766/144‑1 (MQ=255)
                                                                                                                          tcaACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTg                           <  3:111643/149‑1 (MQ=255)
                                                                                                                            aaCATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTggg                         <  8:367006/149‑1 (MQ=255)
                                                                                                                            aaCATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTgg                          <  3:131222/148‑1 (MQ=255)
                                                                                                                            aaCATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAATCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTgg                          <  2:125410/148‑1 (MQ=255)
                                                                                                                             aCATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTgg                          <  1:24973/147‑1 (MQ=255)
                                                                                                                             aCATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTgg                          >  2:24973/1‑147 (MQ=255)
                                                                                                                              cATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGACCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTa                       >  5:394192/1‑149 (MQ=255)
                                                                                                                                tCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGCTAACGAGCGGGTCAATGCCCGGTTGGCTAtt                     >  7:106418/1‑149 (MQ=255)
                                                                                                                                   tGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTcc                  >  7:301680/1‑149 (MQ=255)
                                                                                                                                   tGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTcc                  <  3:346238/149‑1 (MQ=255)
                                                                                                                                     aCCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCgg                <  4:100710/149‑1 (MQ=255)
                                                                                                                                       cAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGt               >  2:342248/1‑148 (MQ=255)
                                                                                                                                        aagaagGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGa             <  2:134714/149‑1 (MQ=255)
                                                                                                                                          gaagGGCGCGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGCCCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAg            >  7:275135/1‑148 (MQ=255)
                                                                                                                                              ggCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAGCAGaa       <  5:354274/149‑1 (MQ=255)
                                                                                                                                              ggCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCAGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAGCAGaa       <  3:348032/149‑1 (MQ=255)
                                                                                                                                                 gAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAGCAGAACg     >  3:350048/1‑148 (MQ=255)
                                                                                                                                                   gCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAGCAGAACGAAg  >  4:324096/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
GAACAGCTCCCGCGGCCTGTCGCGCCAGTTCGACAGCATTTCGCCGTTCAACGTCGAGCGCGTCGAAGTGCTGTCTGGCGCCAGCGC‑CA‑‑TCTACGGCGGTGGCGCCACCGGCGGCATCATCAACATCGTGACCAAGAAGGGCGAGCCTGGCCCGGCGCGCTTCGAAACCCAGTTGGGGGCCAGCAGCGGTTTCAACAACAGCGACGACCTGGCCACCCGCTTCGCCCAGTCCGTCAGCGGTGGTAACGAGCGGGTCAATGCCCGGTTGGGTATTTCCGGTGAGCAGAACGAAG  >  NC_002947/2498750‑2499042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑