Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,682,289 Δ1 bp 7.4% coding (855/1890 nt) prpE → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,682,2890G. 7.4% 170.3 / 7.4 54coding (855/1890 nt)prpEpropionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (24/26);  new base . (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TGTATACCTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTGTTCTGTGCACC  >  NC_002947/2682144‑2682435
                                                                                                                                                  |                                                                                                                                                  
tGTATACCTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTc                                                                                                                                                 <  5:17541/149‑1 (MQ=255)
tGTATACCTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTc                                                                                                                                                 <  1:138055/149‑1 (MQ=255)
 gTATACCTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCg                                                                                                                                                >  6:372068/1‑149 (MQ=255)
   ataCCTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGc                                                                                                                                              >  3:152921/1‑149 (MQ=255)
       cTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGcacct                                                                                                                                          >  1:176803/1‑145 (MQ=255)
       cTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCcact                                                                                                                                          >  1:197580/1‑149 (MQ=255)
       cTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCcact                                                                                                                                          >  8:112064/1‑149 (MQ=255)
       cTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCcact                                                                                                                                          >  7:127758/1‑149 (MQ=255)
        tCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGAG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCcact                                                                                                                                          >  1:81799/1‑148 (MQ=255)
            gCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGT‑GTCGGCCACTCACTGa                                                                                                                                    >  3:211072/1‑149 (MQ=255)
            gCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTAGGTTGGGT‑GTCGGCCACTCACTGa                                                                                                                                    >  3:215999/1‑149 (MQ=255)
               ccaccGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATc                                                                                                                                  >  1:227275/1‑149 (MQ=255)
                caccGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCGTGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCg                                                                                                                                 >  5:192335/1‑149 (MQ=255)
                 accGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGt                                                                                                                                >  5:142373/1‑149 (MQ=255)
                        aGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACggg                                                                                                                         <  4:223607/149‑1 (MQ=255)
                         gCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCca                                                                                                                                            >  5:132125/1‑129 (MQ=255)
                         gCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCca                                                                                                                                            <  6:132125/129‑1 (MQ=255)
                           ccAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGTATTTCCGACGTCGGTTGGGTGGTCGGCcactca                                                                                                                                        >  2:79021/1‑131 (MQ=255)
                            cAAGGGTATCGTCCGGGAAAACGGCGAGCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCg                                                                                                                      >  1:4145/1‑149 (MQ=255)
                             aaGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGTCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGGCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGtt                                                                                                                    <  8:118440/149‑1 (MQ=255)
                                ggTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCGTGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTtgat                                                                                                                 <  6:192335/149‑1 (MQ=255)
                                    tCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGGGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGc                                                                                                             <  1:228364/149‑1 (MQ=255)
                                    tCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAg                                                                                                              <  7:112064/148‑1 (MQ=255)
                                       tCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGc                                                                                                          <  5:372068/149‑1 (MQ=255)
                                       tCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGc                                                                                                          >  6:400785/1‑149 (MQ=255)
                                        ccGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGAGGTCGGTTTGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCt                                                                                                         <  6:298701/149‑1 (MQ=255)
                                         cGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTTCGGGCCGTTGATGAGCGGCTg                                                                                                        >  7:277583/1‑149 (MQ=255)
                                                  ggcg‑gcAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGgt                                                                                               >  2:202143/1‑149 (MQ=255)
                                                    cg‑gcAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGgtgt                                                                                             <  6:52728/149‑1 (MQ=255)
                                                         aaTGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTa                                                                                         <  3:172596/149‑1 (MQ=255)
                                                              cGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAg                                                                                    <  5:373393/149‑1 (MQ=255)
                                                                               ccATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCAcc                                                                   >  7:95168/1‑149 (MQ=255)
                                                                                  catgcCATAGATATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCg                                                                >  2:4698/4‑149 (MQ=255)
                                                                                     gcCATATCTATGGTATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCGACGAAGGCAAGCCAATCCGCACCCCGGAt                                                             >  4:65337/1‑149 (MQ=255)
                                                                                     gcCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGAt                                                             <  1:89308/149‑1 (MQ=255)
                                                                                        atatCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGT‑GTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCtt                                                         <  4:211072/149‑1 (MQ=255)
                                                                                          atCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGT‑GTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGACGGCAAGCCACTCCGCACCCCGGATGCtt                                                         <  4:215999/147‑1 (MQ=255)
                                                                                           tCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTc                                                        <  2:197580/148‑1 (MQ=255)
                                                                                                      cAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGc                                             <  2:81799/148‑1 (MQ=255)
                                                                                                       aGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCgg                                           <  2:4145/149‑1 (MQ=255)
                                                                                                       aGGCCGGGGATGTGCGGTGGGGCAATTCCGACGTCGGATGCGCGGTCGGCCACTCACTGATCGGCTACGGGCCGGTGATCAGCCGCTGTACCACGCTGTTCTACGAACGCAACCCAACCCGCACCCCGGATGCTTCGGCCTATTGGCgg                                           <  6:389698/149‑1 (MQ=255)
                                                                                                         gCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGggt                                         >  3:52252/1‑149 (MQ=255)
                                                                                                          ccGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGTCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGggtg                                        <  2:227275/149‑1 (MQ=255)
                                                                                                           cGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGggtgg                                       <  5:400785/149‑1 (MQ=255)
                                                                                                             gTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGgtggtg                                     <  8:127758/149‑1 (MQ=255)
                                                                                                                aTGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAg                                  <  1:4698/149‑1 (MQ=255)
                                                                                                                   tgtgGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCa                                <  6:142373/148‑1 (MQ=255)
                                                                                                                     tggtggGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAg                                  >  4:170550/1‑144 (MQ=255)
                                                                                                                     tggtggGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAg                                  <  3:170550/144‑1 (MQ=255)
                                                                                                                           ggCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCTGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGt                       <  1:202143/149‑1 (MQ=255)
                                                                                                                                     aCGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTg             >  6:358030/1‑149 (MQ=255)
                                                                                                                                           gTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTGTTCtgt       >  7:16593/1‑149 (MQ=255)
                                                                                                                                           gTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTGTTCtgt       >  7:180174/1‑149 (MQ=255)
                                                                                                                                            ttGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTGTTCtgtg      <  4:52252/149‑1 (MQ=255)
                                                                                                                                               ggttgtCGGCCACTCACTGATCTTCTAAGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGGCAACGCGCTGTTCTGTGCAc   <  2:281181/149‑1 (MQ=255)
                                                                                                                                                gtggtCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTGTTCTGTGCAcc  >  2:111840/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGTATACCTCAGGCACCACCGGTAAGCCCAAGGGTATCGTCCGGGAAAACGGCG‑GCAATGCCGTGGCGCTGTGTTACGCCATGCGCCATATCTATGGCATGCAGGCCGGTGATGTGTGGTGGGGCATTTCCGACGTCGGTTGGGTGGTCGGCCACTCACTGATCGTCTACGGGCCGTTGATGAGCGGCTGTACCACGGTGTTCTACGAAGGCAAGCCAATCCGCACCCCGGATGCTTCGGCCTATTGGCGGGTGGTGGAGCAGTACCGGGTCAACGCGCTGTTCTGTGCACC  >  NC_002947/2682144‑2682435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑