Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,441,069 Δ1 bp 6.2% coding (1515/1653 nt) PP_4780 ← acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,441,0690C. 6.2% 212.1 / 6.5 65coding (1515/1653 nt)PP_4780acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (31/30);  new base . (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

AAGCCCGCTGCTACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCACCCAGTTCGA  >  NC_002947/5440921‑5441217
                                                                                                                                                    |                                                                                                                                                    
aaGCCCGCTGCTACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATGACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTcc                                                                                                                                                      >  8:333975/1‑149 (MQ=255)
 aGCCCGCTGCTACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCca                                                                                                                                                     <  3:105903/149‑1 (MQ=255)
  gCCCGCTGCTACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCcag                                                                                                                                                    >  3:150031/1‑149 (MQ=255)
    ccGCTGCTACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACCGACGTCTGCATTCCCGGCCTCcagca                                                                                                                                                  <  1:205135/149‑1 (MQ=255)
      gctgctACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCcagcagc                                                                                                                                                <  8:336052/149‑1 (MQ=255)
      gctgctACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCcagcag                                                                                                                                                 >  6:88661/1‑148 (MQ=255)
          ctACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCagcagcagc                                                                                                                                             <  7:309744/148‑1 (MQ=255)
          ctACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCagcagcagc                                                                                                                                             <  2:169662/148‑1 (MQ=255)
          ctACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCagcagcagc                                                                                                                                             <  3:271310/148‑1 (MQ=255)
          ctACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCt                                                                                                                                            >  5:228056/1‑149 (MQ=255)
                gggCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCt                                                                                                                                      <  6:141520/149‑1 (MQ=255)
                  gCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTg                                                                                                                                     >  8:92300/1‑148 (MQ=255)
                      ggcaggcGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACg                                                                                                                                >  7:257546/1‑149 (MQ=255)
                           gcGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAgg                                                                                                                           >  2:275005/1‑149 (MQ=255)
                            cGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCTGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGACGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAggg                                                                                                                          <  8:309094/149‑1 (MQ=255)
                                  ccTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAatat                                                                                                                    >  3:276051/1‑149 (MQ=255)
                                   cTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATc                                                                                                                   >  5:12839/1‑149 (MQ=255)
                                      gtgACGAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATcc                                                                                                                  <  5:18573/147‑1 (MQ=255)
                                       tgACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTc                                                                                                                <  6:91737/148‑1 (MQ=255)
                                          ccAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGt                                                                                                             >  3:232691/1‑148 (MQ=255)
                                          ccAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTc                                                                                                            <  1:275005/149‑1 (MQ=255)
                                           cAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCa                                                                                                           <  5:206279/149‑1 (MQ=255)
                                                 cATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCT‑‑A‑CAGCAGCTTGGCCTGCAACGCCAGGGc                                                                                                                         >  3:154412/1‑126 (MQ=255)
                                                 cATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCT‑‑A‑CAGCAGCTTGGCCTGCAACGCCAGGGc                                                                                                                         >  3:272761/1‑126 (MQ=255)
                                                 cATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCT‑‑A‑CAGCAGCTTGGCCTGCAACGCCAGGGc                                                                                                                         <  4:272761/126‑1 (MQ=255)
                                                 cATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCT‑‑A‑CAGCAGCTTGGCCTGCAACGCCAGGGc                                                                                                                         <  4:154412/126‑1 (MQ=255)
                                                  aTCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCcg                                                                                                    <  7:92300/149‑1 (MQ=255)
                                                            cacCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCt                                                                                                                                            <  2:245046/99‑1 (MQ=255)
                                                            cacCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCt                                                                                                                                            >  1:245046/1‑99 (MQ=255)
                                                               cTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCGCTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTg                                                                                        >  2:220428/1‑148 (MQ=255)
                                                               cTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTg                                                                                        >  4:152166/1‑148 (MQ=255)
                                                               cTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTg                                                                                        >  7:163880/1‑148 (MQ=255)
                                                               cTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGc                                                                                       <  7:333975/149‑1 (MQ=255)
                                                                  gcgGCAGCGCACAGAACACCCGCCCACCACCGTACAAGAGTCTTCCTAAAAACGCATCACTGACGTCTGCATACCCGGCCTCCAGCAGCAGCTTTGCCTGCAACTCCAGGGCAATATCCTCTGTCAGTCGCCGCTCGCGGTACTGCAt                                                                                     <  4:290276/148‑1 (MQ=255)
                                                                   cgGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGt                                                                                   <  4:150031/149‑1 (MQ=255)
                                                                    ggCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCAt                                                                                     <  3:349055/146‑1 (MQ=255)
                                                                      cAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCGCTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCAc                                                                                <  1:220428/149‑1 (MQ=255)
                                                                      cAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCAc                                                                                <  4:288726/149‑1 (MQ=255)
                                                                             ccGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTc                                                                         >  4:104280/1‑149 (MQ=255)
                                                                             ccGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTc                                                                         >  1:356241/1‑149 (MQ=255)
                                                                              cGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCg                                                                        <  8:257546/149‑1 (MQ=255)
                                                                                   acCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCaac                                                                   >  1:232455/1‑148 (MQ=255)
                                                                                          caccacCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGccccc                                                            <  3:147473/149‑1 (MQ=255)
                                                                                          acccaccGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGccccc                                                            <  8:163880/147‑1 (MQ=255)
                                                                                           accaccGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCt                                                           >  8:51284/1‑149 (MQ=255)
                                                                                                     aaGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGcc                                                 <  2:375174/149‑1 (MQ=255)
                                                                                                     aaGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGAACTGCATGTCACCGgt                                                                            >  6:51511/1‑122 (MQ=255)
                                                                                                     aaGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGAACTGCATGTCACCGgt                                                                            <  5:51511/122‑1 (MQ=255)
                                                                                                          gCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGatat                                            >  3:234648/1‑149 (MQ=255)
                                                                                                              ccGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCACGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATgcgc                                        >  8:136755/1‑149 (MQ=255)
                                                                                                               cGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATgc                                          <  3:218203/146‑1 (MQ=255)
                                                                                                               cGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATgc                                          >  4:218203/1‑146 (MQ=255)
                                                                                                                gATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGcgccg                                      >  7:370056/1‑149 (MQ=255)
                                                                                                                     aCGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAgg                                 >  6:2119/1‑149 (MQ=255)
                                                                                                                       gCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGgcg                               >  5:42572/1‑149 (MQ=255)
                                                                                                                          tCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCgg                            <  3:152166/149‑1 (MQ=255)
                                                                                                                          tCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCgg                            <  4:232691/149‑1 (MQ=255)
                                                                                                                            aCTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGt                          >  1:1077/1‑149 (MQ=255)
                                                                                                                            aCTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGt                          <  6:12839/149‑1 (MQ=255)
                                                                                                                                aCGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGc                       <  6:228056/148‑1 (MQ=255)
                                                                                                                                 cGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCa                     <  4:276051/149‑1 (MQ=255)
                                                                                                                                  gTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGgcgc                              >  8:215652/1‑139 (MQ=255)
                                                                                                                                  gTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGgcgc                              <  7:215652/139‑1 (MQ=255)
                                                                                                                                     tGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGcc                 >  4:143104/1‑149 (MQ=255)
                                                                                                                                         ttCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGt              >  3:246385/1‑148 (MQ=255)
                                                                                                                                            ccGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGccc                <  8:329177/143‑1 (MQ=255)
                                                                                                                                            ccGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGccc                >  7:329177/1‑143 (MQ=255)
                                                                                                                                             cGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCAcc          >  7:37345/1‑148 (MQ=255)
                                                                                                                                             cGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCAcc          >  8:119743/1‑148 (MQ=255)
                                                                                                                                             cGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCAcc          >  3:203436/1‑148 (MQ=255)
                                                                                                                                                 cTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCACCGCCAGGCGCGGGTCGCCATGCCCGTCACCCAGtt     >  1:378893/1‑149 (MQ=255)
                                                                                                                                                   ccAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCACCCAGTTc    >  7:269495/1‑148 (MQ=255)
                                                                                                                                                    cagcagcagCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCACCCAGTTCGa  >  6:281866/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGCCCGCTGCTACAGGGGCCAGGCAGGCGTAGCCCTTGTGACCAATGCCATCACATTTACACCTCGCGGCAGCGCCCCGTACACCCGGCCACCACCGGCCAAGCGGCTGCCGATAAACGCATCACTGACGTCTGCATTCCCGGCCTCCAGCAGCAGCTTGGCCTGCAACGCCAGGGCAATATCCTCGGTCAGTTGCCGCGCGCGGTACTGCATGTCACCGGTGTCGGCAAACGCCCCCTTGAGGTTGCCGATATGCGCCGCCAGGCGCGGGTCGCCATGCCCGTCACCCAGTTCGA  >  NC_002947/5440921‑5441217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑