BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,300,022:1	+G	6.8%	coding (156/1608 nt)	PP_1134 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,300,022	1	.	G	6.8%	465.7 / 29.1	118	coding (156/1608 nt)	PP_1134	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (53/57); new base G (4/4); total (57/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 123 total aligned reads displayed.

ATCGGGCGCTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTC  >  NC_002947/1299876‑1300170

aTCGGGCGCTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAAcagc‑ag                                                                                                                                                    >  3:385761/1‑149 (MQ=255)

       gcTCCTGCAGGCCTGGCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTg                                                                                                                                             <  2:201327/149‑1 (MQ=255)

       gcTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTg                                                                                                                                             <  2:360130/149‑1 (MQ=255)

         tCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCTGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGtt                                                                                                                                           >  8:231508/1‑149 (MQ=255)

         tCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGtt                                                                                                                                           >  1:103619/1‑149 (MQ=255)

            tGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGttct                                                                                                                                         >  7:351718/1‑148 (MQ=255)

              cAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGCGAGCT‑GCTGttcat                                                                                                                                        <  6:386178/147‑3 (MQ=255)

              cAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGCGAGCT‑GCTGttcat                                                                                                                                        <  6:391407/147‑3 (MQ=255)

              cAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGttcttc                                                                                                                                       <  6:191152/148‑1 (MQ=255)

              cAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGttcttc                                                                                                                                       <  4:321640/148‑1 (MQ=255)

                 gCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGAAGAACAGCGAGCT‑GCTGTtcatctaca                                                                                                                                   <  3:1443/149‑4 (MQ=255)

                   cTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTgcgc                                                                                                                                 >  3:361397/1‑149 (MQ=255)

                   cTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTgcgc                                                                                                                                 <  1:171074/149‑1 (MQ=255)

                    tGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTgcgcg                                                                                                                                >  1:49885/1‑149 (MQ=255)

                    tGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTgcgcg                                                                                                                                >  8:112455/1‑149 (MQ=255)

                      ccGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCa                                                                                                                              >  3:78747/1‑149 (MQ=255)

                       cGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCAt                                                                                                                             >  2:53547/1‑149 (MQ=255)

                            ccTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAg                                                                                                                        <  1:149117/149‑1 (MQ=255)

                               caccGACGAACTTGTAGGTGACGTCTGGCGCCGGGCCGAAGTAGCGATCATCGGCGAACATGCTGAGCAGCTCTGCCTCGTTGCTGCCTTCCAGATCGATAGTGTAGATCAACACC‑AGCTCGCTGTTCTTCATGCGCGCAACGTAGCTg                                                                                                                     >  8:289243/3‑149 (MQ=255)

                                    acgaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCtt                                                                                                                <  4:361397/149‑1 (MQ=255)

                                      gaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTgcgc                                                                                                                  >  1:64464/1‑145 (MQ=255)

                                      gaACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTg                                                                                                               >  4:142768/1‑148 (MQ=255)

                                          ttGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCTGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAg                                                                                                          >  3:144675/1‑149 (MQ=255)

                                          ttGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAg                                                                                                          >  7:229369/1‑149 (MQ=255)

                                            gTAGGTGACGTCTGGGGCTGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAgg                                                                                                         <  7:231508/148‑1 (MQ=255)

                                            gTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAgg                                                                                                         <  4:229129/148‑1 (MQ=255)

                                            gTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGt                                                                                                        >  4:158918/1‑149 (MQ=255)

                                             tAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGtt                                                                                                       <  8:229369/149‑1 (MQ=255)

                                             tAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGt                                                                                                        <  5:284272/148‑1 (MQ=255)

                                                 tGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGaa                                                                                                    <  3:303644/148‑1 (MQ=255)

                                                     gTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGTTGTAGATGAACAGC‑AGCTCTCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTTTAGCTGa                                                                                               <  8:188424/149‑1 (MQ=255)

                                                     gTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCTCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGa                                                                                               <  1:53547/149‑1 (MQ=255)

                                                     gTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGa                                                                                               <  8:351718/149‑1 (MQ=255)

                                                      tCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTCGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAg                                                                                              >  2:83898/1‑149 (MQ=255)

                                                         cgggccgggccGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAg                                                                                           >  8:179419/2‑149 (MQ=255)

                                                          gggccgggccGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATTAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCa                                                                                            <  6:369666/147‑1 (MQ=255)

                                                             ccgggccgATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGc                                                                                       <  3:142768/149‑1 (MQ=255)

                                                              cgggccgATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCt                                                                                      >  7:303197/1‑149 (MQ=255)

                                                                  ccgATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCtcgtc                                                                                  >  2:380358/1‑149 (MQ=255)

                                                                    gATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGGTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTcgc                                                                                <  8:282840/149‑1 (MQ=255)

                                                                    gATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTcgc                                                                                <  5:324968/149‑1 (MQ=255)

                                                                        ttGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGa                                                                             <  2:49885/148‑1 (MQ=255)

                                                                         tGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCt                                                                                                                                              >  8:170354/1‑82 (MQ=255)

                                                                         tGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCt                                                                                                                                              <  7:170354/82‑1 (MQ=255)

                                                                            gATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTg                                                                                                      >  4:773/1‑119 (MQ=255)

                                                                            gATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTg                                                                                                      <  3:773/119‑1 (MQ=255)

                                                                            gATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGt                                                                                          >  5:388312/1‑131 (MQ=255)

                                                                            gATCATCGGCGAACTTGCTGAGAAGCTCGTCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGccc                                                                        <  7:268133/149‑1 (MQ=255)

                                                                              tcatcGGAGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAg                                                                      <  3:209930/149‑1 (MQ=255)

                                                                                ctcGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAgg                                                                     <  4:220100/147‑1 (MQ=255)

                                                                                atcGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAgg                                                                     <  4:333427/148‑1 (MQ=255)

                                                                                   ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGgcgc                                                                  <  7:112455/148‑1 (MQ=255)

                                                                                   ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCt                                                                 >  1:45142/1‑149 (MQ=255)

                                                                                   ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCt                                                                 <  3:372095/149‑1 (MQ=255)

                                                                                   ggCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCt                                                                 <  6:195580/149‑1 (MQ=255)

                                                                                    gCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGgc                                                                    <  8:103738/145‑1 (MQ=255)

                                                                                    gCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGgc                                                                    >  7:103738/1‑145 (MQ=255)

                                                                                     cGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTgg                                                               <  7:289243/149‑1 (MQ=255)

                                                                                          ttGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGAt                                                          >  7:311876/1‑149 (MQ=255)

                                                                                            gCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGAt                                                          <  4:78747/147‑1 (MQ=255)

                                                                                             cTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATgg                                                        <  2:45142/148‑1 (MQ=255)

                                                                                              tGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCt                                                      >  4:244739/1‑149 (MQ=255)

                                                                                                agcagcTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTc                                                    >  6:256676/1‑149 (MQ=255)

                                                                                                    gcTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTcgcg                                                 >  8:189040/1‑148 (MQ=255)

                                                                                                     cTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCa                                               <  6:365258/149‑1 (MQ=255)

                                                                                                        ggCGTCGTTGCTGGCTTCCAGATCGAAGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGGAg                                              <  4:144675/147‑1 (MQ=255)

                                                                                                        ggCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAg                                              <  7:189040/147‑1 (MQ=255)

                                                                                                                agCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCAGCGCTTGAACAGCTTGAATCTGAGAAGTTGCTCGTCGCGGATGCCCAGGCGCTTGACGATGGCTTCGCGCAGCGCTtcgtcg                                    >  5:390111/2‑149 (MQ=255)

                                                                                                                 gCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgtcg                                    <  5:256676/148‑1 (MQ=255)

                                                                                                                     gCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATg                               >  3:332266/1‑149 (MQ=255)

                                                                                                                       ttCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGt                             >  2:224273/1‑149 (MQ=255)

                                                                                                                       ttCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGt                             >  4:161818/1‑149 (MQ=255)

                                                                                                                       ttCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGt                             >  4:96204/1‑149 (MQ=255)

                                                                                                                          cAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTc                            >  4:23302/1‑147 (MQ=255)

                                                                                                                           aGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATgg                                                        >  6:146261/1‑118 (MQ=255)

                                                                                                                           aGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATgg                                                        <  5:146261/118‑1 (MQ=255)

                                                                                                                           aGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgtcg                                    >  1:186487/1‑138 (MQ=255)

                                                                                                                           aGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTtcgtcg                                    <  2:186487/138‑1 (MQ=255)

                                                                                                                            gATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGccc                                                                        <  4:260304/101‑1 (MQ=255)

                                                                                                                            gATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTc                            >  7:350330/1‑145 (MQ=255)

                                                                                                                            gATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTc                            <  8:350330/145‑1 (MQ=255)

                                                                                                                             aTCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCgg                                   <  1:171929/137‑1 (MQ=255)

                                                                                                                             aTCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCgg                                   >  2:171929/1‑137 (MQ=255)

                                                                                                                                gATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAgggg                      >  5:212084/1‑147 (MQ=255)

                                                                                                                                gATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAgggg                      >  3:259195/1‑147 (MQ=255)

                                                                                                                                gATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGc                     <  8:303197/148‑1 (MQ=255)

                                                                                                                                gATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCa                    <  4:332266/149‑1 (MQ=255)

                                                                                                                                 aTGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAg                   >  6:166598/1‑149 (MQ=255)

                                                                                                                                    gtgtAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGACCAGGGGCAGCt                 >  8:43412/1‑148 (MQ=255)

                                                                                                                                       aagaagaACAGCGAGCT‑GCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGAt                                                          <  3:299808/103‑1 (MQ=255)

                                                                                                                                       aagaagaACAGCGAGCT‑GCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAg             >  5:400375/2‑149 (MQ=255)

                                                                                                                                       aagaagaACAGCGAGCT‑GCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAg             >  8:275567/2‑149 (MQ=255)

                                                                                                                                         gaagAACAGCGAGCT‑GCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTGCGCGCAGCGCTTCGTCGGGATGGT‑CTGGGGCAGCTTCAGTTc          >  8:364140/4‑149 (MQ=255)

                                                                                                                                            gAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCgg        >  4:381902/1‑149 (MQ=255)

                                                                                                                                             aaCAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGt       <  3:161818/149‑1 (MQ=255)

                                                                                                                                             aaCAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGt       <  3:247358/149‑1 (MQ=255)

                                                                                                                                                agc‑agcTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGAt    <  3:244739/149‑1 (MQ=255)

                                                                                                                                                agc‑agcTCACTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGAt    >  5:77794/1‑149 (MQ=255)

                                                                                                                                                 gc‑agcTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGAtt   <  6:212084/149‑1 (MQ=255)

                                                                                                                                                  c‑agcTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTc  >  7:68975/1‑149 (MQ=255)

ATCGGGCGCTCCTGCAGGCCTGCCGGGGCCTGGCCGACGAACTTGTAGGTGACGTCTGGGGCCGGGCCGATGTTGCGATCATCGGCGAACTTGCTGAGCAGCTCGGCCTCGTTGCTGGCTTCCAGATCGATGGTGTAGATGAACAGC‑AGCTCGCTGTTCTTCTTGCGCGCATCGTAGCTGCGCTTGAACAGGTTGAAGCTGAGCAGTTGCTCGTCGCGGATGCCCAGGCGCTGGACGATGGCTTCGCGCAGCGCTTCGTCGGGATGGTCCAGGGGCAGCTTCAGTTCGGTGATTC  >  NC_002947/1299876‑1300170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`