Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,320,492:1 +C 5.9% coding (749/945 nt) PP_2041 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,320,4921.C 5.9% 272.6 / 11.1 68coding (749/945 nt)PP_2041LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (27/37);  new base C (2/2);  total (29/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

CAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGCTCGATGCG  >  NC_002947/2320345‑2320637
                                                                                                                                                    |                                                                                                                                                 
cAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAg                                                                                                                                                    <  5:366695/148‑1 (MQ=255)
 aGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑gc                                                                                                                                                 >  3:228747/1‑149 (MQ=255)
  gcgcTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑gcg                                                                                                                                                >  6:316525/1‑149 (MQ=255)
   cgctcgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATAAGCCGCGCGCTGGCAG‑gcgc                                                                                                                                               >  6:331999/1‑149 (MQ=255)
    gctcgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑Gcgcg                                                                                                                                              >  4:215405/1‑149 (MQ=255)
     ctcgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGC‑CTGCCAGCGCGCgg                                                                                                                                             <  5:137682/149‑1 (MQ=255)
       cgctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGcc                                                                                                                                           <  7:160208/149‑1 (MQ=255)
        gctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGcc                                                                                                                                           <  5:316525/148‑1 (MQ=255)
         ctGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGc                                                                                                                                            >  7:92665/1‑146 (MQ=255)
            cgcACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGC‑CTGCCAGCGCGCGGctgata                                                                                                                                       <  4:123724/148‑6 (MQ=255)
             gcACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATcc                                                                                                                                     <  8:29521/149‑1 (MQ=255)
             gcACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATcc                                                                                                                                     >  6:352570/1‑149 (MQ=255)
              cACCGCATCCACCAGCCGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCca                                                                                                                                    <  6:258459/149‑1 (MQ=255)
              cACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCca                                                                                                                                    <  8:344232/149‑1 (MQ=255)
               aCCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGAGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCcag                                                                                                                                   >  4:330128/1‑149 (MQ=255)
                 cGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCcagca                                                                                                                                 <  1:115598/149‑1 (MQ=255)
                       caccaGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAgcggcg                                                                                                                           <  7:270475/149‑1 (MQ=255)
                       caccaGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAgcggc                                                                                                                            <  2:362405/148‑1 (MQ=255)
                        cacacccgTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGcggcgg                                                                                                                          <  5:397723/142‑1 (MQ=255)
                             cagTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGt                                                                                                                     >  3:279103/1‑149 (MQ=255)
                             cagTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGt                                                                                                                     >  3:282519/1‑149 (MQ=255)
                                   gcgcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGa                                                                                                               <  3:148158/149‑1 (MQ=255)
                                    cgcACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAT‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGAt                                                                                                              <  2:252217/149‑1 (MQ=255)
                                        ccAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGAtggtg                                                                                                          >  6:13152/1‑149 (MQ=255)
                                            gTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGgccg                                                                                                      >  3:115540/1‑149 (MQ=255)
                                            gTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGgccg                                                                                                      >  1:280941/1‑149 (MQ=255)
                                               gcgcATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgcca                                                                                                   <  7:268561/149‑1 (MQ=255)
                                                cgcATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGGTGGTGGCCgccag                                                                                                  <  4:228747/149‑1 (MQ=255)
                                                 gcATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccagg                                                                                                 >  2:209507/1‑149 (MQ=255)
                                                  cATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCgccaggc                                                                                                >  2:338993/1‑149 (MQ=255)
                                                          cggcggcgAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACgg                                                                                        <  4:279103/149‑1 (MQ=255)
                                                          cggcggcgAACGACAGCGGCTTGGGCTGCGAGGGGCCCAAGGCGATGGTGCGGGCGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACgg                                                                                        <  4:115540/149‑1 (MQ=255)
                                                            gcggcgAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCTCCAGGCCAGCACGGAc                                                                                      <  2:256382/149‑1 (MQ=255)
                                                            gcggcgAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGAc                                                                                      <  2:250899/149‑1 (MQ=255)
                                                             cggcgAACGACAGCTGATTGTGCTGCGTGTGGGCCAAGGGTATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCTGCCAGATCCAGCTGCTGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGAct                                                                                     <  5:69888/149‑2 (MQ=255)
                                                                     gACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGt                                                                             <  8:92665/149‑1 (MQ=255)
                                                                      aCAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGt                                                                             >  6:107807/1‑148 (MQ=255)
                                                                              ttGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCggg                                                                    >  3:102569/1‑149 (MQ=255)
                                                                                 tgctgaGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGc                                                                 <  1:264668/149‑1 (MQ=255)
                                                                                    tgcGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGa                                                              >  1:241648/1‑149 (MQ=255)
                                                                                      cGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGa                                                              <  4:282519/147‑1 (MQ=255)
                                                                                      cGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGAct                                                            >  4:287852/1‑149 (MQ=255)
                                                                                         gTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGActgct                                                         <  4:102569/149‑1 (MQ=255)
                                                                                         gTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGActgct                                                         <  5:83110/149‑1 (MQ=255)
                                                                                             gCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTc                                                     >  4:7280/1‑149 (MQ=255)
                                                                                             gCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTc                                                     <  5:13152/149‑1 (MQ=255)
                                                                                              ccAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCg                                                    >  3:164511/1‑149 (MQ=255)
                                                                                               cAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGt                                                   <  1:338993/149‑1 (MQ=255)
                                                                                                aaGGCGATGGTGCGGGGGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGGGATACCGGGGGCCGACTGCTGTTCGTa                                                  <  2:266466/149‑1 (MQ=255)
                                                                                                 aGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAg                                                 >  5:235033/1‑149 (MQ=255)
                                                                                                    cGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTg                                              <  3:7280/149‑1 (MQ=255)
                                                                                                          tGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTc                                         >  3:290662/1‑148 (MQ=255)
                                                                                                           gCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCa                                       <  7:267810/149‑1 (MQ=255)
                                                                                                             gggTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAgt                                     >  4:364734/1‑149 (MQ=255)
                                                                                                              ggTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAgtg                                    >  5:81892/1‑149 (MQ=255)
                                                                                                                  aTGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTcac                                <  5:352570/149‑1 (MQ=255)
                                                                                                                      gcgGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCg                            <  3:287852/149‑1 (MQ=255)
                                                                                                                         gTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCa                                       >  3:390617/1‑135 (MQ=255)
                                                                                                                         gTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCa                                       <  4:390617/135‑1 (MQ=255)
                                                                                                                            ccACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTc                       >  2:113171/1‑148 (MQ=255)
                                                                                                                             cACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCa                     <  6:318745/149‑1 (MQ=255)
                                                                                                                              aCTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCAt                    >  1:362374/1‑149 (MQ=255)
                                                                                                                                tATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATgg                  <  3:215405/149‑1 (MQ=255)
                                                                                                                                   tggCCGCGC‑CTGCCAGCGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGCCTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAg               >  3:91951/3‑149 (MQ=255)
                                                                                                                                   tggCCGCGC‑CTGCCAGCGCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAg               >  4:10888/3‑149 (MQ=255)
                                                                                                                                       cgcgcgCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTg           >  7:260510/1‑149 (MQ=255)
                                                                                                                                        gcgcgcTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGc          <  3:330128/149‑1 (MQ=255)
                                                                                                                                        gcgcgcTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGc          >  2:316595/1‑149 (MQ=255)
                                                                                                                                         cgcgcTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGCt         <  3:364734/149‑1 (MQ=255)
                                                                                                                                         cgcgcTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGCt         <  2:280941/149‑1 (MQ=255)
                                                                                                                                             cTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGCTCGAt     <  1:316595/149‑1 (MQ=255)
                                                                                                                                                gCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACATCGGTTTCCATGGCAGCCTGCTCGATgcg  >  1:160632/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGCGCTCGCTGCGCACCGCATCCACCAGCAGTGCGCGCACCAGGTCGCGCATGCAGGCGGCGGCGAACGACAGCGGCTTGTGCTGCGAGTGGGCCAAGGCGATGGTGCGGGTGATGCGCGGTTCCACTATCAGCCGCGCGCTGGCAG‑GCGCGGCCAGATCCAGCAGCGGCGGCCAGTTGCTGATGGTGGCCGCCAGGCCAGCACGGACCAGGCTGGTGATACCGGGGGCCGACTGCTGTTCGTAGCTGGAGTCCAGTGTCACACCGGTTTCCATGGCAGCCTGCTCGATGCG  >  NC_002947/2320345‑2320637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑