Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,622,933	T→A	7.3%	M269L (ATG→TTG) ‡	PP_4941 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,622,933	0	T	A	7.3%	204.2 / 4.7	68	M269L (ATG→TTG) ‡	PP_4941	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (28/35);  new base A (3/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

AAGGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAG  >  NC_002947/5622785‑5623081                                                                                                                                                     |                                                                                                                                                     aaGGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCAt                                                                                                                                                      >  3:284629/1‑149 (MQ=255)    gCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggc                                                                                                                                                   <  2:26905/149‑1 (MQ=255)     cTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggca                                                                                                                                                  <  7:324983/149‑1 (MQ=255)     cTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggca                                                                                                                                                  <  7:204357/149‑1 (MQ=255)      tCGCAGGTCGGCCAGCAGGTTGTGACAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCGGGTCGGGGGCTGCCGGCTTgagag                                                                                                                                                 >  7:244969/1‑145 (MQ=255)        gCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggcagg                                                                                                                                                <  1:87061/148‑1 (MQ=255)        gCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATggcagg                                                                                                                                                >  2:228659/1‑148 (MQ=255)         cAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCCAtgcaggca                                                                                                                                              <  5:9121/149‑1 (MQ=255)              cGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTg                                                                                                                                          >  1:94377/1‑148 (MQ=255)               ggCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCCATGCAGGCAGcaccc                                                                                                                                        <  3:62614/149‑5 (MQ=255)               ggCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCa                                                                                                                                        <  6:119017/149‑1 (MQ=255)                 ccAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAgg                                                                                                                                      <  8:340361/149‑1 (MQ=255)                      agGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCaaaa                                                                                                                                 <  5:158853/149‑1 (MQ=255)                       ggTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAg                                                                                                                                <  2:307912/149‑1 (MQ=255)                        gTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAg                                                                                                                                >  4:149378/1‑148 (MQ=255)                         ttGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAg                                                                                                                                >  4:150434/1‑147 (MQ=255)                         ttGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTa                                                                                                                              <  1:228659/149‑1 (MQ=255)                            tgCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGcc                                                                                                                           <  7:56363/149‑1 (MQ=255)                               cAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAg                                                                                                                        >  7:34380/1‑149 (MQ=255)                                 ggtgggtgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCt                                                                                                                      <  8:334559/149‑1 (MQ=255)                                      gtgCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATg                                                                                                                 >  5:85668/1‑149 (MQ=255)                                         cgcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCaa                                                                                                              <  4:218109/149‑1 (MQ=255)                                          gcCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGTCCAGGTAGGCTTCAAGTGAGGCGTCCGGCTGGTAGGGTGCTTCCTGAATTGAAGGAAGCTGAAGGCAATAGTAGCACATGTGTATTCAAc                                                                                                             >  6:295047/1‑149 (MQ=255)                                            cATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAAcgc                                                                                                           >  4:340264/1‑149 (MQ=255)                                            cATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAAcgc                                                                                                           <  6:305424/149‑1 (MQ=255)                                            cATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAAcgc                                                                                                           <  8:237013/149‑1 (MQ=255)                                             aTCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGcc                                                                                                                           <  7:338531/132‑1 (MQ=255)                                             aTCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGcc                                                                                                                           >  8:338531/1‑132 (MQ=255)                                              tCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGa                                                                                                         <  3:149378/149‑1 (MQ=255)                                                gcgGTCGCCCGGGAACGGGAACGGGGTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGAc                                                                                                        <  4:284629/148‑1 (MQ=255)                                                                     cgggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAAcgcg                                                                                  <  6:85668/149‑1 (MQ=255)                                                                      gggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCgg                                                                                 <  2:94377/149‑1 (MQ=255)                                                                       ggTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCgg                                                                                           >  3:217839/1‑138 (MQ=255)                                                                       ggTTGAACTGGCGGAACACGCGGATATCGGCAAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCgg                                                                                 >  1:240190/1‑148 (MQ=255)                                                                              cTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAAcgcg                                                                                  <  4:114524/140‑1 (MQ=255)                                                                              cTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAAcgcg                                                                                  >  3:114524/1‑140 (MQ=255)                                                                                 gCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGAt                                                                        <  2:240190/147‑1 (MQ=255)                                                                                 gCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATg                                                                       <  6:333905/148‑1 (MQ=255)                                                                                         cgcgGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTTCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAg                                                              <  5:149221/149‑1 (MQ=255)                                                                                            ggATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCa                                                           >  5:313159/1‑149 (MQ=255)                                                                                                atCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGc                                                                                          <  7:25433/114‑1 (MQ=255)                                                                                                atCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGc                                                                                          >  8:25433/1‑114 (MQ=255)                                                                                                   ggCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGgc                                                    >  3:326749/1‑149 (MQ=255)                                                                                                    gCCAGGTCGACTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGgcg                                                   >  6:338990/1‑149 (MQ=255)                                                                                                     ccAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAAc                                                                                                             <  2:249878/90‑1 (MQ=255)                                                                                                     ccAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAAc                                                                                                             >  1:249878/1‑90 (MQ=255)                                                                                                          tCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCt                                              >  2:88029/1‑148 (MQ=255)                                                                                                          tCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGAAGCTGTCCCAGGCCCGCCGGCGCCCCtt                                             >  8:60346/1‑149 (MQ=255)                                                                                                             gCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAg                                          <  4:326749/149‑1 (MQ=255)                                                                                                                  aCGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGgcgcg                                     >  8:93177/1‑149 (MQ=255)                                                                                                                            gccggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGCCAACGGCACGCCGGCCCAACCCGGCCCGCCCATGCTGTACCCGGCCCCCACGCGCCCCGTCAGGCGCCcggccaccgg                           >  2:387269/1‑144 (MQ=255)                                                                                                                            gccggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcggc                           <  4:178305/149‑1 (MQ=255)                                                                                                                             ccggcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCGGCAGGCAAAAGTAGCCCAGCTGAATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctcggcc                          <  5:295047/149‑1 (MQ=255)                                                                                                                                gcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCCGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCAACAGGCGCGCGGCCTCGGCCttt                       <  7:60346/149‑1 (MQ=255)                                                                                                                                gcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCttt                       <  8:34380/149‑1 (MQ=255)                                                                                                                                gcTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCttt                       >  7:92291/1‑149 (MQ=255)                                                                                                                                  tGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGc                     >  5:176181/1‑149 (MQ=255)                                                                                                                                  tGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGc                     >  1:27929/1‑149 (MQ=255)                                                                                                                                    gTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGc                     <  2:27929/147‑1 (MQ=255)                                                                                                                                    gTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGc                     <  7:93177/147‑1 (MQ=255)                                                                                                                                     tCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGAATCCGGCACGCCGGCCCAACGCGGCACGACGAAGCTGTCCCAGGCACGCAGGCGACCCTTAAGGCGCGCGGCCTCCGCCTTTGCCAg                  >  1:111455/1‑149 (MQ=255)                                                                                                                                      ctgcagctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAgg                 >  1:129941/6‑149 (MQ=255)                                                                                                                                      cGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctc                              <  3:135277/136‑1 (MQ=255)                                                                                                                                      cGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGcggcctc                              >  4:135277/1‑136 (MQ=255)                                                                                                                                      cGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAgg                 <  3:150434/149‑1 (MQ=255)                                                                                                                                          agctgcCTGCCATGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCa             >  6:102334/2‑149 (MQ=255)                                                                                                                                            ctgcctgcCATGCAGGCAGCTGCAGGCAAAAGTAGCACCGCTGTATGCAACGCGCCTCCGGCACGCCGGCCCAACGCGGCACGCCGGTGCTGTCCCAGGCCCGCAGGCGCCCCTTCAGGCGCGCGGCCTCCGCCTTTGCCAGGCCCAtg           >  1:261935/1‑148 (MQ=255)                                                                                                                                               cctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgc        >  5:57386/1‑149 (MQ=255)                                                                                                                                                ctgcATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATctgct       <  3:340264/149‑1 (MQ=255)                                                                                                                                                     tGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAg  <  8:92291/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     AAGGCTCGCAGGTCGGCCAGCAGGTTGTGCCAGGTGGGTGCGCCATCGCGGTCGCCCGGTAACGGGAACGGGTTGAACTGGCGGAACACGCGGATATCGGCCAGGTCGGCTTCACGTGAGGCGGCCGGCTGGTCGGGTGCTGCCTGCATGGCAGGCAGCTGCAGGCAAAAGTAGCCCAGCTGTATGCAACGCGACTCCGGCACGCCGGCCCAACGCGGCACGACGATGCTGTCCCAGGCACGCAGGCGCCCCTTCAGGCGCGCGGCCTCGGCCTTTGCCAGGCCCATCTGCTGGTAG  >  NC_002947/5622785‑5623081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑