Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,643,236 A→G 5.9% intergenic (+21/‑102) dbpA → / → yhiN 23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,643,2360AG 5.9% 253.8 / 4.7 85intergenic (+21/‑102)dbpA/yhiN23S rRNA specific ATP‑dependent RNA helicase/FAD/NAD(P)‑binding domain‑containing oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (38/41);  new base G (2/3);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

GGCAAGATCGCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCT  >  NC_002947/5643090‑5643384
                                                                                                                                                    |                                                                                                                                                    
ggCAAGATCGCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAgg                                                                                                                                                    <  4:58404/149‑1 (MQ=255)
 gCAAGATCGCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGGAAAGCC‑ATGCAGCGGCTGAACAGCCGCAAGATAAACGGTACCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCAGTAg                                                                                                                                                     >  1:309968/1‑148 (MQ=255)
  cAAGATCGCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAg                                                                                                                                                  >  7:106201/1‑149 (MQ=255)
      aTCGCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCggg                                                                                                                                              <  6:61219/149‑1 (MQ=255)
         gCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGttt                                                                                                                                           <  8:119306/149‑1 (MQ=255)
         gCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGttt                                                                                                                                           <  5:337294/149‑1 (MQ=255)
           cATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAAGGCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCATGGTTCGCGCCATCAAAGTCCGCATTATCTAAAGCCAGCCAAAGCCCCCTTAAGGACCGGTTTTa                                                                                                                                          >  1:289637/1‑149 (MQ=255)
            aTCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTAcc                                                                                                                                        <  4:140099/149‑1 (MQ=255)
               ttCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCcgc                                                                                                                                     <  7:310320/149‑1 (MQ=255)
                tCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCCAAGGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCcgc                                                                                                                                     >  1:97599/1‑149 (MQ=255)
                 cGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGGACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCcgc                                                                                                                                     <  4:388615/147‑1 (MQ=255)
                        cAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCgg                                                                                                                             <  3:125522/148‑1 (MQ=255)
                        cAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCgg                                                                                                                             <  2:384115/148‑1 (MQ=255)
                           gCGGTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGt                                                                                                                         <  4:108303/149‑1 (MQ=255)
                                  tgGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCa                                                                                                                  >  4:45794/1‑149 (MQ=255)
                                   ggCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCAc                                                                                                                 >  3:108285/1‑149 (MQ=255)
                                      cGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCAt                                                                                                              >  5:336597/1‑149 (MQ=255)
                                       gTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTATGAGCTGGTTTACCCGCGAATGCGGCAGTGAATGCACCATc                                                                                                             <  7:13918/149‑1 (MQ=255)
                                       gTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATc                                                                                                             >  1:25727/1‑149 (MQ=255)
                                        tGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCg                                                                                                            >  1:252574/1‑149 (MQ=255)
                                        tGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCg                                                                                                            <  5:52780/149‑1 (MQ=255)
                                         ggAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCg                                                                                                            >  8:44264/1‑148 (MQ=255)
                                              gggCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTcg                                                                                                      <  3:371789/149‑1 (MQ=25)
                                               ggCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTTAACATCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCAAATGCGGCAGTGAATGCACCATCGCATTcgc                                                                                                     <  8:138578/149‑1 (MQ=255)
                                               ggCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTcgc                                                                                                     <  2:309968/149‑1 (MQ=255)
                                                gcgcTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTcgcg                                                                                                    >  4:44529/1‑149 (MQ=255)
                                                 cgcTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                   <  6:336597/149‑1 (MQ=255)
                                                 agcTGGCGAA‑GATTGCC‑ATGCATCGGCTTAACAGCGGCAAGATCAAGGGGCGCGACCTGAAAGTGCTCATTATCTAAATCCAGGCAAAGCCCCCTGTGGGAGCGGGTTGACCCGAGAATGCGATGGTGCATTCACTGCCGCATTcgctg                                                                                                   <  8:133543/148‑3 (MQ=12)
                                                       ccAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAc                                                                                             >  2:202149/1‑149 (MQ=255)
                                                       ccAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAc                                                                                             <  3:45794/149‑1 (MQ=255)
                                                        cAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCgg                                                                                                   <  7:50781/142‑1 (MQ=25)
                                                        cAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCgg                                                                                                   >  8:50781/1‑142 (MQ=25)
                                                        cAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAcc                                                                                            >  2:373674/1‑149 (MQ=255)
                                                            gCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                         >  8:283190/1‑149 (MQ=255)
                                                            gCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGc                                                                                         <  2:130312/149‑1 (MQ=255)
                                                                 cc‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCAAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCca                                                                                    <  2:40121/149‑1 (MQ=255)
                                                                  c‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAc                                                                                             >  2:200788/1‑139 (MQ=255)
                                                                  c‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAAc                                                                                             <  1:200788/139‑1 (MQ=255)
                                                                    aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCTCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCcaca                                                                                  <  8:115899/149‑1 (MQ=255)
                                                                    aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCcaca                                                                                  <  7:283190/149‑1 (MQ=255)
                                                                    aTGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCcaca                                                                                  <  1:202149/149‑1 (MQ=255)
                                                                       cAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAggg                                                                               <  1:373674/149‑1 (MQ=255)
                                                                       cAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAgg                                                                                <  2:132604/148‑1 (MQ=255)
                                                                       cAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAgg                                                                                <  5:226744/148‑1 (MQ=255)
                                                                            gCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCAAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACGGGGTTCAg                                                                          <  2:329500/149‑1 (MQ=255)
                                                                               gAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAt                                                                       >  3:264391/1‑149 (MQ=255)
                                                                                aaCAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                      >  3:384050/1‑149 (MQ=255)
                                                                                   aGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                      <  1:343683/146‑1 (MQ=255)
                                                                                   aGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAtt                                                                      >  2:343683/1‑146 (MQ=255)
                                                                                      ggCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCgg                                                                                                   >  2:173688/1‑114 (MQ=255)
                                                                                         aaGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCt                                                              >  5:52130/1‑148 (MQ=255)
                                                                                          aGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTg                                                             >  8:206057/1‑148 (MQ=255)
                                                                                           gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTg                                                             >  4:313725/1‑147 (MQ=255)
                                                                                           gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGaa                                                           <  8:227852/149‑1 (MQ=255)
                                                                                           gATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGaa                                                           <  4:108285/149‑1 (MQ=255)
                                                                                             tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATc                                                               >  5:70067/1‑143 (MQ=255)
                                                                                             tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATc                                                               <  6:70067/143‑1 (MQ=255)
                                                                                             tCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAgg                                                         >  1:238357/1‑149 (MQ=255)
                                                                                                       cgcCCGTAAAGTCCTCATTATATAATGCCTTGAAAAGCCGCCTGTCGCAGCGGGTTTACCGGCTACTGCGGAAGTTAATGCACCATCGATTTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGt                                               <  8:129975/149‑1 (MQ=255)
                                                                                                         cccTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                     <  1:308236/125‑1 (MQ=255)
                                                                                                         cccTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCAttt                                                                     >  2:308236/1‑125 (MQ=255)
                                                                                                           cTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCAc                                           >  6:157536/1‑149 (MQ=255)
                                                                                                                   ccGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGa                                   >  8:64363/1‑149 (MQ=255)
                                                                                                                   ccGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGa                                   >  2:36696/1‑149 (MQ=255)
                                                                                                                    cGCATTATCTACAGCCCGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                  <  8:142113/149‑1 (MQ=255)
                                                                                                                    cGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                  <  2:252574/149‑1 (MQ=255)
                                                                                                                    cGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                  <  2:97599/149‑1 (MQ=255)
                                                                                                                    cGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                  <  8:106201/149‑1 (MQ=255)
                                                                                                                     gCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACg                                 >  6:292921/1‑149 (MQ=255)
                                                                                                                             tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                  <  5:165969/140‑1 (MQ=255)
                                                                                                                             tAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGAc                                  >  6:165969/1‑140 (MQ=255)
                                                                                                                                aGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCtt                      <  7:44264/149‑1 (MQ=255)
                                                                                                                                 gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                     <  7:206057/149‑1 (MQ=255)
                                                                                                                                 gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                     <  4:265702/149‑1 (MQ=255)
                                                                                                                                 gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                     >  1:245981/1‑149 (MQ=255)
                                                                                                                                 gCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                     >  7:208628/1‑149 (MQ=255)
                                                                                                                                  ccAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAAGCTGAAGGTTCATACCGTGCCCTCCACCGACGTGATCAGCCTTgg                    >  6:376765/1‑149 (MQ=255)
                                                                                                                                    aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGCTCATCCTTggcg                  >  8:332950/1‑149 (MQ=255)
                                                                                                                                    aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                     <  1:266789/146‑1 (MQ=255)
                                                                                                                                    aGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTg                     >  2:266789/1‑146 (MQ=255)
                                                                                                                                      gCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgcc                >  2:127891/1‑149 (MQ=255)
                                                                                                                                          tgaacccTGTGGGAGCGGGTTCACCCGCGAATGCGATGGTGCATTCACTGCCGCATTCGCGGGTAAACCCGCTCCTACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTggcgccggcg            >  8:371717/5‑149 (MQ=17)
                                                                                                                                             ccccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATCCACCATCGCATTAGCGGGTGAACACGCTCCCACAGGGTTCACCATTTCAAATCTCAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGgcgccggcgccg         >  4:13002/1‑149 (MQ=38)
                                                                                                                                               ccTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCgccgcc       >  1:383944/1‑149 (MQ=255)
                                                                                                                                                   tAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGc    <  7:297901/148‑1 (MQ=255)
                                                                                                                                                    aGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCt  >  6:239948/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCAAGATCGCCATCTTCGACTTCCAGGCGTTTGTGGCCGTGGAGCGGGCGCTGGCCAA‑GCAGGCC‑ATGCAGCGGCTGAACAGCGGCAAGATCAAGGGTCGCGCCCTGAAAGTCCGCATTATCTAAAGCCAGGCAAAGCCCCCTGTAGGAGCGGGTTTACCCGCGAATGCGGCAGTGAATGCACCATCGCATTCGCGGGTGAACCCGCTCCCACAGGGTTCAGCATTTCAAATCTGAAGGTTCATACCGTGCACTCCACCGACGTGATCATCCTTGGCGCCGGCGCCGCCGGCCT  >  NC_002947/5643090‑5643384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑