Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	418,296	C→G	5.2%	Q27E (CAG→GAG)	PP_0345 →	heptose kinase WapP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	418,296	0	C	G	5.2%	249.9 / 3.4	77	Q27E (CAG→GAG)	PP_0345	heptose kinase WapP
Reads supporting (aligned to +/- strand):  ref base C (38/35);  new base G (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

TCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCAA  >  NC_002947/418151‑418442                                                                                                                                                  |                                                                                                                                                   tCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAgg                                                                                                                                                 >  8:154642/1‑149 (MQ=255)   gCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGc                                                                                                                                               >  1:195329/1‑149 (MQ=255)         ctggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                         <  2:319837/149‑1 (MQ=255)          tggAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGc                                                                                                                                        >  7:304594/1‑149 (MQ=255)           ggAGCGCAAGGCCCAGAAACTCTACGCGCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcaccg                                                                                                                                       <  6:336310/149‑5 (MQ=255)              gcgcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgac                                                                                                                                    >  4:68014/1‑149 (MQ=255)                gcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGTGAACGGCTgaccc                                                                                                                                  >  1:165207/1‑149 (MQ=255)                gcAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgaccc                                                                                                                                  >  1:236795/1‑149 (MQ=255)                  aaGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                         >  4:304084/1‑140 (MQ=255)                  aaGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACgg                                                                                                                                         <  3:304084/140‑1 (MQ=255)                       ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgaccc                                                                                                                           <  8:304594/149‑1 (MQ=255)                       ccAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTAGCCCTCCACGGCGAACGGCTgacccgcgcccc                                                                                                                           >  2:395152/1‑149 (MQ=255)                         aGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcaccg                                                                                                                                       >  5:194769/1‑131 (MQ=255)                         aGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTGGAGGGCGAAcaccg                                                                                                                                       <  6:194769/135‑5 (MQ=255)                         aGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTgacccgcgacccgc                                                                                                                         >  8:20506/1‑149 (MQ=255)                            aaCTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCa                                                                                                                      <  3:367926/149‑1 (MQ=255)                               tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGt                                                                                                                    >  7:168419/1‑148 (MQ=255)                               tctACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGt                                                                                                                    >  5:19914/1‑148 (MQ=255)                                 tACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCa                                                                                                                      >  3:341383/1‑144 (MQ=255)                                 tACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCa                                                                                                                      <  4:341383/144‑1 (MQ=255)                                 tACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGaa                                                                                                                 >  7:222891/1‑149 (MQ=255)                                   cGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAg                                                                                                                <  7:1909/148‑1 (MQ=255)                                    gATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTg                                                                                                              <  1:43235/149‑1 (MQ=255)                                        gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTTTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTa                                                                                                           <  6:345449/148‑1 (MQ=255)                                        gcaagcaaCGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTAc                                                                                                          >  3:37059/1‑149 (MQ=255)                                                 gCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc                                                                                                 <  2:236795/149‑1 (MQ=255)                                                 gCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGc                                                                                                 <  3:374972/149‑1 (MQ=255)                                                       gggATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACg                                                                                           >  8:342023/1‑149 (MQ=255)                                                        ggATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACgg                                                                                          <  4:37059/149‑1 (MQ=255)                                                         gATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACgg                                                                                          >  1:382137/1‑148 (MQ=255)                                                          aTGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGa                                                                                        <  3:68014/149‑1 (MQ=255)                                                             cACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATc                                                                                      <  5:219742/148‑1 (MQ=255)                                                               ctctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                   <  2:195329/149‑1 (MQ=255)                                                                tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                   >  4:200393/1‑148 (MQ=255)                                                                tctGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                   >  3:150959/1‑148 (MQ=255)                                                                  tGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAact                                                                                  <  4:150959/147‑1 (MQ=255)                                                                    aTGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAacta                                                                                 <  1:395152/146‑1 (MQ=255)                                                                       gCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGt                                                                           >  2:104434/1‑149 (MQ=255)                                                                        cGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTc                                                                          <  8:222891/149‑1 (MQ=255)                                                                               gACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCt                                                                   <  7:342023/149‑1 (MQ=255)                                                                                   cTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTacac                                                               >  1:13246/1‑149 (MQ=255)                                                                                   cTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGCGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTacac                                                               <  7:132214/149‑1 (MQ=255)                                                                                    tGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACAcc                                                              >  7:30125/1‑149 (MQ=255)                                                                                        gcCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCgggg                                                          >  5:3600/1‑149 (MQ=255)                                                                                          cGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGAc                                                                                            >  7:207545/1‑113 (MQ=255)                                                                                          cGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGAc                                                                                            <  8:207545/113‑1 (MQ=255)                                                                                           ggggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCg                                                       <  7:126624/149‑1 (MQ=255)                                                                                           ggggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCACTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGTTTAACCACTACGTCAAGCGCTACACCgggg                                                          <  3:49354/146‑1 (MQ=255)                                                                                           gcggaaCGAACAACTGGCGGCGGACTTCGGCAGCCACGACGCGGTGTTCGCCCACCAGGGCGAACGGCTCACCAGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATTAACCACTACGTCAACCTCTACACCgggc                                                          >  4:49354/6‑145 (MQ=255)                                                                                             ggTACGAACACCTGGCGGCTGACTTCGGCAGTCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGc                                                     <  6:297143/149‑1 (MQ=255)                                                                                             ggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGc                                                     <  5:185082/149‑1 (MQ=255)                                                                                             ggTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGc                                                     <  6:216857/149‑1 (MQ=255)                                                                                              gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCTCAATCCAGGCCGAACGGCTGACCCGCCACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGAAGGCATCaaa                                                                                   >  4:329882/1‑117 (MQ=255)                                                                                              gTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAac                                                                                   <  3:329882/118‑1 (MQ=255)                                                                                                  gAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGca                                                >  3:154680/1‑149 (MQ=255)                                                                                                      acCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATg                                            >  8:319211/1‑149 (MQ=255)                                                                                                           gcggcgGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCg                                       <  3:200393/149‑1 (MQ=255)                                                                                                                      cGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCCAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggc                            >  7:153267/1‑149 (MQ=255)                                                                                                                         cAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGggcggc                         <  8:168419/149‑1 (MQ=255)                                                                                                                           gCCTCGACGCGGTGTTCGCCCTCCAGGGTGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCa                       <  2:165207/149‑1 (MQ=255)                                                                                                                             cTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCcgcg                     >  5:299366/1‑149 (MQ=255)                                                                                                                             cTCGACGCGGTGTTAGCCCTCCAGGGCGAACTGCTGACCCGCGACCCGCTCAGTTAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACg                     >  4:58908/1‑149 (MQ=255)                                                                                                                              tCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACACGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTTCACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACg                     >  1:259414/1‑148 (MQ=255)                                                                                                                                   gcgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATc                 >  4:29274/1‑147 (MQ=255)                                                                                                                                    cgGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAg              >  2:100818/1‑149 (MQ=255)                                                                                                                                     ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg             <  7:154642/149‑1 (MQ=255)                                                                                                                                     ggTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAgg             >  2:127174/1‑149 (MQ=255)                                                                                                                                       tgtTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCaa               >  7:100591/1‑145 (MQ=255)                                                                                                                                       tgtTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCaa               <  8:100591/145‑1 (MQ=255)                                                                                                                                       tgtTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGcc           <  6:3600/149‑1 (MQ=255)                                                                                                                                       cgtTCGCCCTGGAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCCCGCATCAAGGcc           >  7:347719/2‑149 (MQ=255)                                                                                                                                           cGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt       >  7:70107/1‑149 (MQ=255)                                                                                                                                            gCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAAt       <  6:19914/148‑1 (MQ=255)                                                                                                                                            gCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATg      >  4:130454/1‑149 (MQ=255)                                                                                                                                              ccTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc                                         <  7:234208/112‑1 (MQ=255)                                                                                                                                              ccTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATgcgc                                         >  8:234208/1‑112 (MQ=255)                                                                                                                                              ccTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGc    <  3:29274/149‑1 (MQ=255)                                                                                                                                                 ccAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCaa  >  8:2669/1‑148 (MQ=255)                                                                                                                                                  cAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCg                                       >  2:70715/1‑110 (MQ=255)                                                                                                                                                  cAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCg                                       <  1:70715/110‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TCGCCTGGCTGGAGCGCAAGGCCCAGAAACTCTACGATCGCAAGCAACGCTATGGGGATGCACTCTGATGGCGAGTTGGACACTGGCGCCGGGGTACGAACACCTGGCGGCGGACTTCGGCAGCCTCGACGCGGTGTTCGCCCTCCAGGGCGAACGGCTGACCCGCGACCCGCTCAGTGAAGTGGTACGCATCGAGCGTGACGGGATCAACTACTACGTCAAGCGCTACACCGGGGCCGGCAAGCACATGCGCCGGTACCTGGGGCGGCCACGCATCAAGGCCGAATGGCAA  >  NC_002947/418151‑418442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑