Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,651,904 G→T 5.2% A189D (GCC→GAC)  fba ← fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,651,9040GT 5.2% 241.5 / 3.0 77A189D (GCC→GAC) fbafructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (37/36);  new base T (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

GGTACCGAGGACGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGTTCGCCTT  >  NC_002947/5651757‑5652051
                                                                                                                                                   |                                                                                                                                                   
ggTCCCGAGGACGACCCGGGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGc                                                                                                                                                    <  8:99067/149‑1 (MQ=255)
     cgacgaCGCACCGTGCAGCACCAGGTGGGGGTCGGGGATGCGCTTGTGGATTTCCTTGATACGCTCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGa                                                                                                                                               <  8:368684/149‑1 (MQ=255)
      gAGGACGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGa                                                                                                                                               >  5:231523/1‑148 (MQ=255)
          aCGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTgg                                                                                                                                          <  5:328957/149‑1 (MQ=255)
          aCGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTgg                                                                                                                                          <  2:200813/149‑1 (MQ=255)
          aCGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTgg                                                                                                                                          <  2:674/149‑1 (MQ=255)
          aCGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTgg                                                                                                                                          <  7:195449/149‑1 (MQ=255)
            gAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGTCGACGCCCTgg                                                                                                                                          >  5:159593/1‑147 (MQ=255)
            gAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGTCGACGCCCTgg                                                                                                                                          <  1:94975/147‑1 (MQ=255)
            gAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGTCGACGCCCTgg                                                                                                                                          <  6:159593/147‑1 (MQ=255)
            gAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGTCGACGCCCTgg                                                                                                                                          >  2:94975/1‑147 (MQ=255)
            gAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGt                                                                                                                                        <  1:110576/149‑1 (MQ=255)
               ccGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGtct                                                                                                                                      >  5:49355/1‑148 (MQ=255)
                  tGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTcttctt                                                                                                                                  >  4:236351/1‑149 (MQ=255)
                  tGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTcttctt                                                                                                                                  >  2:194684/1‑149 (MQ=255)
                  tGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTcttctt                                                                                                                                  <  7:313133/149‑1 (MQ=255)
                    cATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTcttctt                                                                                                                                  >  3:289019/1‑147 (MQ=255)
                    cATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTcttctt                                                                                                                                  <  4:289019/147‑1 (MQ=255)
                      tCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGAc                                                                                                                               >  4:368114/1‑148 (MQ=255)
                        aCCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACg                                                                                                                              >  3:277790/1‑147 (MQ=255)
                        aCCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACg                                                                                                                              >  1:327661/1‑147 (MQ=255)
                          cAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGTTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATAGCCAGGGCTGAGACCTGGGTCTTCTTGACGAAGt                                                                                                                          >  7:268159/1‑149 (MQ=255)
                                 gtgtTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCggcggc                                                                                                                   <  8:257467/149‑1 (MQ=255)
                                          aTGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCgg                                                                                                          <  7:221746/149‑1 (MQ=255)
                                              gcTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCGTGACGAAGTCGGCGGCTTCTTCCGggtcg                                                                                                      >  5:347118/1‑149 (MQ=255)
                                              gcTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTAAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGAAAGGGCGTCGACCTGGGTCTACTTGACGAAGACCGCGGCTTCTTCCCggtcg                                                                                                      >  7:188287/1‑149 (MQ=255)
                                                ttGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGggtcgg                                                                                                     >  1:362383/1‑148 (MQ=255)
                                                   tgGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAg                                                                                                 >  8:103761/1‑149 (MQ=255)
                                                     gATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCa                                                                                               >  6:35408/1‑149 (MQ=255)
                                                      aTTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCa                                                                                               >  1:317486/1‑148 (MQ=255)
                                                           cTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTgg                                                                                          <  3:215059/148‑1 (MQ=255)
                                                           cTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGc                                                                                         >  2:219584/1‑149 (MQ=255)
                                                           cTTGATACGATCGATGGCCAGCAAGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTgg                                                                                          <  4:101505/148‑1 (MQ=255)
                                                            ttGATACGATCGATGGCCAGCACGTCACCGGATGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGCCCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCt                                                                                        >  4:190527/1‑149 (MQ=255)
                                                              gATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCtg                                                                                       <  2:45756/148‑1 (MQ=255)
                                                                 aCGATCGATGGTCAGCACGTCACCGGTTGGTGGCTTGCTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGAt                                                                                   >  1:68995/1‑149 (MQ=255)
                                                                  cgatcgatGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGAt                                                                                   <  4:230992/148‑1 (MQ=255)
                                                                   gatcgatGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCa                                                                                                  >  4:371687/1‑132 (MQ=255)
                                                                   gatcgatGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCa                                                                                                  <  3:371687/132‑1 (MQ=255)
                                                                    atcgatGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCa                                                                                <  2:317486/149‑1 (MQ=255)
                                                                    atcgatGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCa                                                                                <  3:236351/149‑1 (MQ=255)
                                                                            ccAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGa                                                                                    >  5:159487/1‑137 (MQ=255)
                                                                            ccAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGa                                                                                    <  6:159487/137‑1 (MQ=255)
                                                                                cACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCgg                                                                                                          <  4:282905/111‑1 (MQ=255)
                                                                                cACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCgg                                                                                                          >  3:282905/1‑111 (MQ=255)
                                                                                   gTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCgg                                                                    <  7:317798/146‑1 (MQ=255)
                                                                                   gTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCgg                                                                    >  8:317798/1‑146 (MQ=255)
                                                                                   gTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGgcgc                                                                 >  7:324082/1‑149 (MQ=255)
                                                                                     cACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGcc                                                                >  6:291558/1‑148 (MQ=255)
                                                                                      aCCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGa                                                              >  5:156243/1‑149 (MQ=255)
                                                                                             ggtggCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGA‑CCAGCACGCCTTCGGCGCCGATCCCGtc                                                       <  5:122009/148‑1 (MQ=255)
                                                                                                ggCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGtcttc                                                    <  8:324082/149‑1 (MQ=255)
                                                                                                 gCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGtcttct                                                   <  3:368114/149‑1 (MQ=255)
                                                                                                       tGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCg                                             <  6:156243/149‑1 (MQ=255)
                                                                                                            ttGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCAt                                        >  2:164701/1‑149 (MQ=255)
                                                                                                                  gCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGcc                                                                >  8:285272/1‑119 (MQ=255)
                                                                                                                  gCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGcc                                                                <  7:285272/119‑1 (MQ=255)
                                                                                                                  gCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGt                                  >  6:301270/1‑149 (MQ=255)
                                                                                                                    aCCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGttt                                <  2:68995/149‑1 (MQ=255)
                                                                                                                    aCCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGttt                                >  2:365801/1‑149 (MQ=255)
                                                                                                                      cGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTcc                              <  4:191177/149‑1 (MQ=255)
                                                                                                                      cGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTc                               >  7:345765/1‑148 (MQ=255)
                                                                                                                       gTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGGCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCa                             <  6:49355/149‑1 (MQ=255)
                                                                                                                        tGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGTCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAg                            <  6:231523/149‑1 (MQ=255)
                                                                                                                                aCCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGcc                      >  5:124457/1‑147 (MQ=255)
                                                                                                                                  cGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAgg                  >  4:379671/1‑149 (MQ=255)
                                                                                                                                  cGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGACCATGCCGGTTTCCAGGGAGCCCAgg                  <  2:327661/149‑1 (MQ=255)
                                                                                                                                  cGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGGCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGACCATGCCGGTTTCCAGGGAGCCCAgg                  <  4:277790/149‑1 (MQ=255)
                                                                                                                                   gATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGc                 >  7:228589/1‑149 (MQ=255)
                                                                                                                                         gATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGcc             >  7:50514/1‑147 (MQ=255)
                                                                                                                                         gATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGcc             >  7:189422/1‑147 (MQ=255)
                                                                                                                                         gATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGcc             >  3:325771/1‑147 (MQ=255)
                                                                                                                                           tCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGt         <  8:189422/149‑1 (MQ=255)
                                                                                                                                              ccAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGTTCg      >  7:125009/1‑149 (MQ=255)
                                                                                                                                               cAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGGCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGTTCGc     <  7:103761/149‑1 (MQ=255)
                                                                                                                                                aGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGTTCGcc    <  2:362383/149‑1 (MQ=255)
                                                                                                                                                aGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGTTCGcc    <  6:347118/149‑1 (MQ=255)
                                                                                                                                                   gCGTCGACATGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCATCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTACCACGGACCCCAGGCAGCCCACTTCGCCtt  >  4:206606/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTACCGAGGACGAACCGTGCATCACCAGGTGGGTGTTGGGGATGCGCTTGTGGATTTCCTTGATACGATCGATGGCCAGCACGTCACCGGTTGGTGGCTTGGTGAACTTGTAGGCACCGTGGCTGGTACCGATGGCGATCGCCAGGGCGTCGACCTGGGTCTTCTTGACGAAGTCGGCGGCTTCTTCCGGGTCGGTCAGCATCTGGCTGTGATCCAGCACGCCTTCGGCGCCGATCCCGTCTTCTTCACCGGCCATGCCGGTTTCCAGGGAGCCCAGGCAGCCCAGTTCGCCTT  >  NC_002947/5651757‑5652051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑