Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,657,893	C→A	6.4%	intergenic (‑199/‑53)	tktA ← / → sahR	transketolase A/methionine metabolism transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,657,893	0	C	A	6.4%	191.2 / 3.6	63	intergenic (‑199/‑53)	tktA/sahR	transketolase A/methionine metabolism transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base A (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

ATTTTCGCCCACTGGGGGCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGC  >  NC_002947/5657745‑5658041                                                                                                                                                     |                                                                                                                                                     aTTTTCGCCCACTGGGGGCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAGCTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAAc                                                                                                                                                      >  5:362395/1‑149 (MQ=255)      cGCCCACTGGGGGCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACtttt                                                                                                                                                  <  8:213756/148‑1 (MQ=255)          cACTGGGGGCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGata                                                                                                                                             <  1:383037/149‑1 (MQ=255)          cACTGGGGGCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAAAGTTTTGata                                                                                                                                             >  3:133928/1‑149 (MQ=255)               ggggCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAAAGTTTTGATATTgg                                                                                                                                         <  4:133928/148‑1 (MQ=255)               ggggCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAAAGTTTTGATATTGGc                                                                                                                                        <  2:210915/149‑1 (MQ=255)                cggcgcttggcgcttAAATGTTTGCCCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGGGTATCTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCATAACTTTTTGATATTGGcc                                                                                                                                       >  7:23216/2‑149 (MQ=38)                        ggggCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAgg                                                                                                                               >  8:316187/1‑149 (MQ=255)                          ggCAAACATTTAAGCGCCAAGCGCCGGTTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAggg                                                                                                                              >  7:57443/1‑148 (MQ=255)                          ggCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGACGCTTTCGGCTTGCAGCTCATCGCCAAAATCAAAACTTTTTGATATTGGCATTGCTAggg                                                                                                                              >  3:66283/1‑148 (MQ=255)                             aaaCATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACtttt                                                                                                                                                  >  1:275529/1‑125 (MQ=255)                             aaaCATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACtttt                                                                                                                                                  <  2:275529/125‑1 (MQ=255)                             aaaCATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATc                                                                                                                          >  3:196912/1‑149 (MQ=255)                               aCATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGa                                                                                                                        >  5:296972/1‑149 (MQ=255)                                  tttAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTACCTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGc                                                                                                                     <  6:362395/149‑1 (MQ=255)                                     aagcgccaagcgccGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCt                                                                                                                   <  7:316187/148‑1 (MQ=255)                                         gccaagcgccGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTa                                                                                                              <  8:40843/149‑1 (MQ=255)                                           caagcgccGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATc                                                                                                                          <  3:82529/135‑1 (MQ=255)                                           caagcgccGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATc                                                                                                                          >  4:82529/1‑135 (MQ=255)                                               cgccGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTgc                                                                                                        <  5:69559/149‑1 (MQ=255)                                                 cagtcTTTCAGCCGCAACTGAAGAAGCAGAGCGCGACTCTGGGCTCCCCAAAGCTTTTAACTAGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTgccg                                                                                                      <  4:219501/145‑1 (MQ=255)                                                  cGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTgccgc                                                                                                     >  8:221430/1‑149 (MQ=255)                                                  cGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTgccgc                                                                                                     >  5:334555/1‑149 (MQ=255)                                                      tttAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCa                                                                                                 <  5:318508/149‑1 (MQ=255)                                                             cGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCt                                                                                          >  5:160126/1‑149 (MQ=255)                                                             cGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCt                                                                                          <  6:334555/149‑1 (MQ=255)                                                                    gaagaaGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCtgcgtgcg                                                                                   >  4:97326/1‑149 (MQ=255)                                                                       gaagCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTgccgc                                                                                                     <  8:78714/128‑1 (MQ=255)                                                                       gaagCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTgccgc                                                                                                     >  7:78714/1‑128 (MQ=255)                                                                        aagCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAAt                                                                               <  1:361120/149‑1 (MQ=255)                                                                            agagCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGAt                                                                           <  7:120308/149‑1 (MQ=255)                                                                             gagCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCCAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATc                                                                          >  7:28519/1‑149 (MQ=255)                                                                                   gACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAg                                                                    >  4:321136/1‑149 (MQ=255)                                                                                   gACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAg                                                                    <  6:160126/149‑1 (MQ=255)                                                                                        ggggCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAAcg                                                               <  3:321136/149‑1 (MQ=255)                                                                                         gggCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAAcgc                                                              <  6:296972/149‑1 (MQ=255)                                                                                               cccAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCTGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACa                                                        >  8:344037/1‑149 (MQ=255)                                                                                                 cAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACAtt                                                      <  4:196912/149‑1 (MQ=255)                                                                                                 cAAAGCGTGGAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGCCTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACAtt                                                      <  8:28519/149‑1 (MQ=255)                                                                                                  aaaGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCTGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTg                                                     <  7:344037/149‑1 (MQ=255)                                                                                                     gCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCa                                                  <  8:57443/149‑1 (MQ=255)                                                                                                         gtAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGccc                                              >  3:161256/1‑149 (MQ=255)                                                                                                           aaCTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGc                                                <  5:86965/145‑1 (MQ=255)                                                                                                           aaCTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGc                                                >  6:86965/1‑145 (MQ=255)                                                                                                             cTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCtgtg                                          <  7:221430/149‑1 (MQ=255)                                                                                                              ttGAGTCTTTCGGCTTGCAGCTAATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGc                                         <  8:266853/149‑1 (MQ=255)                                                                                                                  ggCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGCAGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCGGCGCAACGCGAAACATTGGCAGCCCTTTGCAAAg                                     >  6:163042/1‑149 (MQ=255)                                                                                                                     tttCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGc                                    >  1:345941/1‑147 (MQ=255)                                                                                                                       tCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCa                                  <  2:345941/147‑1 (MQ=255)                                                                                                                         ggCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCACCGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTgg                              >  7:184634/1‑149 (MQ=255)                                                                                                                          gCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGc                             >  5:323006/1‑149 (MQ=255)                                                                                                                          gCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGc                             <  5:124110/149‑1 (MQ=255)                                                                                                                              gCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGa                           <  7:24776/147‑1 (MQ=255)                                                                                                                                     aTCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGAtgcgctgcg                  >  6:9015/1‑149 (MQ=255)                                                                                                                                       ggCCAATATCAAAAAGTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGcc                >  1:379578/2‑149 (MQ=255)                                                                                                                                        gCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGcc                <  5:9015/148‑1 (MQ=255)                                                                                                                                         ccAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGATTCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGCTGCGCTGTGCCTg              >  5:274014/1‑149 (MQ=255)                                                                                                                                           aaTATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGaa            <  3:249841/149‑1 (MQ=255)                                                                                                                                            atatCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAAc           >  1:230069/1‑149 (MQ=255)                                                                                                                                                 aaaaCTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTg                                                     <  8:114466/102‑1 (MQ=255)                                                                                                                                                 aaaaCTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTg                                                     >  7:114466/1‑102 (MQ=255)                                                                                                                                                 aaaaCTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTAtt      >  5:118618/1‑149 (MQ=255)                                                                                                                                                 aaaaCTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTAtt      >  8:114077/1‑149 (MQ=255)                                                                                                                                                 aaaaCTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTAtt      >  8:307566/1‑149 (MQ=255)                                                                                                                                                     cTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTgcac  >  1:293889/1‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ATTTTCGCCCACTGGGGGCGCTTGGGGCAAACATTTAAGCGCCAAGCGCCGGCTTTAAGCCGCAACTGAAGAAGCAGAGCGCGACTCGGGGCGCCCCAAAGCGTGTAACTTGAGGCTTTCGGCTTGCAGCTCATCGCCAATATCAAAACTTTTTGATATTGGCCTTGCTAGGGCATCGATGCCTGTCTAGACTGCCGCCCCATGAATCTGCGTGCGCAATCGATCCCCGAGCAACGCGAAACATTGGCAGCCCTGTGCAAAGCCAGTGGCGATGCGCTGCGCCTGAACGTATTGCGC  >  NC_002947/5657745‑5658041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑