Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,725,646 T→G 7.0% K440N (AAA→AAC)  opgH ← glucans biosynthesis glucosyltransferase H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,725,6460TG 7.0% 173.8 / 3.7 59K440N (AAA→AAC) opgHglucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base G (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

CTTGAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACT  >  NC_002947/5725505‑5725791
                                                                                                                                             |                                                                                                                                                 
cTTGAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCg                                                                                                                                            <  5:95615/149‑1 (MQ=255)
 ttGAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGACCGGCAAGTCGTAGGCAATCCACACCCCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCgg                                                                                                                                           >  5:269918/1‑149 (MQ=255)
       tCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACgg                                                                                                                                     >  4:169078/1‑149 (MQ=255)
          tCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgc                                                                                                                                  <  7:252620/149‑1 (MQ=255)
          tCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGACGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGgcgc                                                                                                                                  >  6:284071/1‑149 (MQ=255)
            aaGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGcc                                                                                                                                 <  4:57341/148‑1 (MQ=255)
             aGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAg                                                                                                                               >  6:138252/1‑149 (MQ=255)
                aGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgcgt                                                                                                                                >  6:61610/1‑143 (MQ=255)
                aGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCGTTGCCGGGCAAAGgcgcgt                                                                                                                                <  5:61610/145‑3 (MQ=255)
                  gTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGgcg                                                                                                                           >  6:24893/1‑148 (MQ=255)
                  gTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGAACCGGCGAACGCGCCTTTGCCCGGCACCGGCGCCAGGgcga                                                                                                                          >  6:301829/1‑148 (MQ=255)
                   tttGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCa                                                                                                                         <  5:294769/149‑1 (MQ=255)
                         ggcAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTc                                                                                                                   >  2:394257/1‑149 (MQ=255)
                         ggcAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTc                                                                                                                   >  8:340703/1‑149 (MQ=255)
                           cAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGa                                                                                                                 <  1:95454/149‑1 (MQ=255)
                            aGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGAt                                                                                                                <  3:241392/149‑1 (MQ=255)
                                cttcGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGaa                                                                                                             <  7:340703/148‑1 (MQ=255)
                                 ttcGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACg                                                                                                           >  7:78677/1‑149 (MQ=255)
                                   cGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCt                                                                                                                                                   >  2:49786/1‑107 (MQ=255)
                                   cGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCt                                                                                                                                                   <  1:49786/107‑1 (MQ=255)
                                   cGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACgg                                                                                                          <  4:66505/148‑1 (MQ=255)
                                    gTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACTCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCt                                                                                                        <  3:169078/149‑1 (MQ=255)
                                     tAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCtt                                                                                                       <  6:202993/149‑1 (MQ=255)
                                           cccGGCAAGTCGTAGTCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGc                                                                                                  <  4:62206/148‑1 (MQ=255)
                                                      gTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGt                                                                                      <  5:284071/149‑1 (MQ=255)
                                                       tAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGtt                                                                                     >  3:78590/1‑149 (MQ=255)
                                                         ggCAATCCACACCCCCCAGCCGTCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtgt                                                                                   <  6:269918/149‑1 (MQ=255)
                                                          gCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTtgtg                                                                                  <  3:357390/149‑1 (MQ=255)
                                                            aaTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                               <  8:138079/134‑1 (MQ=255)
                                                            aaTCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGga                                                                                               >  7:138079/1‑134 (MQ=255)
                                                               ccACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGccc                                                                              >  1:295756/1‑148 (MQ=255)
                                                                 acacacCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCag                                                                           >  6:366715/1‑149 (MQ=255)
                                                                       cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAAc                                                                                                            <  7:58817/110‑1 (MQ=255)
                                                                       cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAAc                                                                                                            >  8:58817/1‑110 (MQ=255)
                                                                       cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGc                                                                     >  5:136916/1‑149 (MQ=255)
                                                                       cccAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGc                                                                     >  8:177253/1‑149 (MQ=255)
                                                                       cacAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGACGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCCACGGCGCCAGGGCGCAGTTCTCGAGGAACGGATTCATGAGGAGGATCGCGTTGTGGCCCCAGTAGTGc                                                                     >  3:285878/3‑149 (MQ=255)
                                                                         cAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGa                                                                   <  5:24893/149‑1 (MQ=255)
                                                                         cAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGAGGATCGCGTTGTGGCCCCAGTAGTGCGa                                                                   <  1:18803/149‑1 (MQ=255)
                                                                            ccGGCACGCCGCAGCCGCGCAGCTTCGACGAAGCCGTGGGACAGGACGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGCCGCAGCGCTCGAGGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTc                                                                <  8:370886/149‑1 (MQ=255)
                                                                              ggCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCAc                                                              <  7:177253/149‑1 (MQ=255)
                                                                                      gcATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGc                                                      <  2:36578/149‑1 (MQ=255)
                                                                                        aTCAGCGCAGCTTCGTAGTAGTCGTGGGAAAGGTTGTCCCCGGCGAACGAGCCTTTGCCCGGCTACGGCGCCAGGGCGCAGTGCTCGATGATCGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCTACTTCCa                                                    <  4:285878/149‑1 (MQ=255)
                                                                                           aGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGaa                                                 <  5:366715/149‑1 (MQ=255)
                                                                                           aGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGaa                                                 <  1:394257/149‑1 (MQ=255)
                                                                                           aGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCATTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCCGTAGTGCGACTACCCCAACTGCCAg                                                   >  1:92974/1‑147 (MQ=255)
                                                                                            gcgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGTAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCa                                                                            <  1:289420/121‑1 (MQ=255)
                                                                                            gcgcAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGTAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCa                                                                            >  2:289420/1‑121 (MQ=255)
                                                                                                aGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCa                                            <  5:123623/149‑1 (MQ=255)
                                                                                                             tagTGGGAAAGGATGGCACCGGCGAACGCGCCTTT‑CCCGGCAACGGCGCCATGGCGCAGTGCTCGATGAACGGCTTCATGCCGATGATCGAGTTGTGGCCCCAGTAGTGCGACTCACCACACTGCCAAAAGTTCAGACCGGCAGTGaa                               >  4:296382/3‑148 (MQ=255)
                                                                                                             tCGTGGGAAAGGATGGCACCGGCGAACGCGCC‑TTGCCCGGCAACGGCGCCAGGGCCCAGTGCTCGATGAACGGCTTCATGCGGATGATAGCGTTGTTGCCCCAGTATTGCGACTCACCCAACTGACAGAAGTGCAGACCGGCACTGaa                               >  4:291133/1‑148 (MQ=255)
                                                                                                                  ggAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTg                          <  3:380896/149‑1 (MQ=255)
                                                                                                                   gAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTgg                         >  6:82089/1‑149 (MQ=255)
                                                                                                                    aaaGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTggg                        <  2:295756/149‑1 (MQ=255)
                                                                                                                     aaGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGc                       >  2:227145/1‑149 (MQ=255)
                                                                                                                       ggATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCg                     >  3:14199/1‑149 (MQ=255)
                                                                                                                        gATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCg                     >  1:56351/1‑148 (MQ=255)
                                                                                                                                    gAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAg        >  3:192189/1‑149 (MQ=255)
                                                                                                                                    gAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAg        >  3:186030/1‑149 (MQ=255)
                                                                                                                                      tggcgcCGTTGCCGGGCAAAGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACGCGCGGGTGGc       >  8:248181/3‑148 (MQ=255)
                                                                                                                                          gcCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAAct  >  5:290010/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                 
CTTGAGCTCGTCAAGCAGGTTTGGCGGCAGTTCTTCGTAGCTGCCCGGCAAGTCGTAGGCAATCCACACACCCCAGCCGGCACGGCGCATCAGCGCAGCTTCGACGAAGTCGTGGGAAAGGATGGCACCGGCGAACGCGCCTTTGCCCGGCAACGGCGCCAGGGCGCAGTGCTCGATGAACGGCTTCATGCGGATGATCGCGTTGTGGCCCCAGTAGTGCGACTCACCCAACTGCCAGAAGTGCAGACCGGCAGTGAACAGTGGGCCGTACACGCGGGTAGCGAACT  >  NC_002947/5725505‑5725791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑