Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,743,352	G→T	9.2%	intergenic (‑185/+62)	fbp ← / ← glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,743,352	0	G	T	9.2%	229.1 / 8.2	74	intergenic (‑185/+62)	fbp/glgP	class 1 fructose‑1,6‑bisphosphatase/glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (36/31);  new base T (4/3);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

ACGCAGTTTACAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATA  >  NC_002947/5743204‑5743499                                                                                                                                                      |                                                                                                                                                    aCGCAGTTTACAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAg                                                                                                                                                     >  7:218237/1‑149 (MQ=255)          aCAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGcc                                                                                                                                             <  2:3062/148‑1 (MQ=255)            aGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCtt                                                                                                                                           >  3:202238/1‑148 (MQ=255)             gggAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGa                                                                                                                                         <  7:199141/149‑1 (MQ=255)              ggAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTg                                                                                                                                          >  1:258396/1‑147 (MQ=255)              ggAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTg                                                                                                                                          >  7:147660/1‑147 (MQ=255)              ggAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTg                                                                                                                                          >  2:375856/1‑147 (MQ=255)               gagaGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGaa                                                                                                                                        <  7:286797/148‑1 (MQ=255)               gagaGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAg                                                                                                                                       >  4:313315/1‑149 (MQ=255)                     gACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGc                                                                                                                                  <  8:40846/148‑1 (MQ=255)                       cGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTAGAGCGCAAGCCTTGAAGCTTGCga                                                                                                                                <  6:323901/148‑1 (MQ=255)                       cGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCga                                                                                                                                <  8:147660/148‑1 (MQ=255)                           cTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCGTTCGCGGGCACGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCgctgtac                                                                                                                           <  5:97857/149‑7 (MQ=21)                            tAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACAcc                                                                                                                          >  8:171899/1‑149 (MQ=255)                                 aGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGTCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTgg                                                                                                                     <  6:320911/149‑1 (MQ=21)                                   tgatgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCCCGCTCCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGa                                                                                                                   <  2:168572/149‑1 (MQ=21)                                     atgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCCAATCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGa                                                                                                                   <  1:324073/148‑1 (MQ=255)                                     atgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGc                                                                                                                 >  1:370381/1‑149 (MQ=255)                                     atgaGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TACGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTAGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGc                                                                                                                 >  1:96476/1‑149 (MQ=255)                                        agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCgg                                                                                                               <  5:6296/148‑1 (MQ=255)                                        agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCgg                                                                                                               <  1:375856/148‑1 (MQ=255)                                        agaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCACTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCgg                                                                                                               <  5:42945/148‑1 (MQ=255)                                         gaCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGTGAGACACCTGTGGGAGCGGGc                                                                                                             <  8:317617/149‑1 (MQ=255)                                         gaCTCGCCACGGCTCTCAGACGGCACAATAGGGCGGTAGTTCAGCC‑TTCGCCAGGCCGGTCGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGc                                                                                                             <  2:153006/149‑1 (MQ=255)                                          aCTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGAC‑TTCGCCCGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGGACATCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGTCGCGGGCg                                                                                                            >  5:204470/1‑149 (MQ=255)                                              gCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGc                                                                                                         <  1:30000/148‑1 (MQ=255)                                              gCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGc                                                                                                         <  6:28416/148‑1 (MQ=255)                                                   ggCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCcgcg                                                                                                    >  8:289833/1‑148 (MQ=255)                                                        tcAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCGCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGc                                                                                              >  3:46627/1‑149 (MQ=255)                                                          aGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCGCCCACCGTTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGc                                                                                              <  3:54336/147‑1 (MQ=255)                                                           gACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCg                                                                                                                                 <  3:54597/111‑1 (MQ=255)                                                           gACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCg                                                                                                                                 >  4:54597/1‑111 (MQ=255)                                                            aCGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctca                                                                                          >  2:37896/1‑149 (MQ=255)                                                              ggCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGcctcacc                                                                                        >  4:336332/1‑149 (MQ=255)                                                                   aaTAGGTCGGTAGTTCAGCC‑TTCACCAGGCTGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAAt                                                                                   <  2:66869/149‑1 (MQ=255)                                                                       ggTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCaa                                                                               <  2:96476/149‑1 (MQ=255)                                                                        gTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAt                                                                              <  7:171899/149‑1 (MQ=255)                                                                        gTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAAt                                                                              <  7:289833/149‑1 (MQ=255)                                                                         tCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATg                                                                             <  3:313315/149‑1 (MQ=255)                                                                          cGGTAGTTCAGCC‑TGCGCCAGGCCGGTGGTCTGTTGGTGTGTCCATTCGCGGGCGCGGTCGCCCCCACTGGTACTGCGCAAGCGTTGAAGCTTGCGAGACACCTGTGGGAGGGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGc                                                                            <  7:155470/149‑1 (MQ=255)                                                                                ttCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTcc                                                                       >  8:206184/1‑148 (MQ=255)                                                                                     cc‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACAGCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGAtct                                                                 <  2:258396/149‑1 (MQ=255)                                                                                           gCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTTAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGt                                                             <  4:164785/148‑1 (MQ=255)                                                                                            ccAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTgg                                                           >  4:129706/1‑149 (MQ=255)                                                                                            ccAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTgg                                                           <  8:218237/149‑1 (MQ=255)                                                                                                    gtggtCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcg                                                   >  3:70388/1‑149 (MQ=255)                                                                                                     tggtCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTcgc                                                  <  3:129706/149‑1 (MQ=255)                                                                                                            gTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAGCGGCCGCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGt                                           >  1:197251/1‑149 (MQ=255)                                                                                                            gTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCGCCTCACTGCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATggg                                           >  5:165103/1‑148 (MQ=255)                                                                                                            gTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGt                                           >  6:44865/1‑149 (MQ=255)                                                                                                            gTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGt                                           >  4:10983/1‑149 (MQ=255)                                                                                                               gtgtgtCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCg                                        >  2:65143/1‑149 (MQ=255)                                                                                                                   gtCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTcc                                    >  7:357448/1‑149 (MQ=255)                                                                                                                   gtCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTcc                                    >  2:51443/1‑149 (MQ=255)                                                                                                                       aTTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                 >  2:355598/1‑148 (MQ=255)                                                                                                                       aTTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                 >  6:126978/1‑148 (MQ=255)                                                                                                                       aTTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTGCCAGATCTCGGTGGCATATTCGAGACTGGTGCGGTAcgac                                 >  6:220390/1‑148 (MQ=255)                                                                                                                       aTTCGCGGGCGCGCTCGCT‑CCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTCGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCcgac                                 >  8:210593/1‑147 (MQ=255)                                                                                                                        ttCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCg                                        <  5:148228/140‑1 (MQ=255)                                                                                                                        ttCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCg                                        >  6:148228/1‑140 (MQ=255)                                                                                                                             gggCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTg                                          >  1:208923/1‑133 (MQ=255)                                                                                                                             gggCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTg                                          <  2:208923/133‑1 (MQ=255)                                                                                                                               gcgcgcTCGCTCCCACAGGTACAGCCCAATCCTTCAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCTCGAACGGCCTCACCTCAATCCAATGCCTTCCAGTTCTCGTTGGCATATTCTCCTTTGTTGCGGTCCGACGAGAACCAg                        <  6:165103/149‑1 (MQ=255)                                                                                                                                 gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACgag                              >  7:15732/1‑141 (MQ=255)                                                                                                                                 gcgcTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACgag                              <  8:15732/141‑1 (MQ=255)                                                                                                                                   gcccgctcCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCACTCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGccc                     >  7:209922/4‑148 (MQ=18)                                                                                                                                    ctcgctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCAt                   >  8:119084/1‑149 (MQ=255)                                                                                                                                     tcgctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATc                  >  5:239103/1‑149 (MQ=255)                                                                                                                                       gctcCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGAGTTCCCGCGAACGGACTAACCTCAATCCAATGCCGTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCAGACGAGAACCAGCACATccg                >  5:276760/1‑149 (MQ=255)                                                                                                                                         tcCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGAGCGAGCGCGCCCGCGAATGGACACACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtg              >  5:111876/1‑149 (MQ=17)                                                                                                                                         tcCCACAGGTGTCTCGCAAGCTTCAAGGCTTGCGCTGTACCTGTGGGAGCGAGCGCGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtg              >  5:256017/1‑149 (MQ=18)                                                                                                                                             acaGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATccgtgccgt          >  2:376175/1‑149 (MQ=255)                                                                                                                                                  tACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTc      >  3:155752/1‑148 (MQ=255)                                                                                                                                                  tACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCa     <  8:99991/149‑1 (MQ=255)                                                                                                                                                     aGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATa  >  7:48674/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                    ACGCAGTTTACAGGGAGAGTGACGGGCTAGCGAGTGATGAGACTCGCCACGGCTCTCAGACGGCACAATAGGTCGGTAGTTCAGCC‑TTCGCCAGGCCGGTGGTCTGGTTGTGTGTCCATTCGCGGGCGCGCTCGCTCCCACAGGTACAGCGCAAGCCTTGAAGCTTGCGAGACACCTGTGGGAGCGGGCGTGCCCGCGAACGGCCTCACCTCAATCCAATGCCTTCCAGATCTCGGTGGCATATTCGCGAATGGTGCGGTCCGACGAGAACCAGCCCATCCGTGCCGTGTTCAATA  >  NC_002947/5743204‑5743499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑