Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,747,458 A→T 6.0% A172A (GCT→GCA)  PP_5043 ← PhoPQ‑activated pathogenicity‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,747,4580AT 6.0% 204.4 / 2.8 67A172A (GCT→GCA) PP_5043PhoPQ‑activated pathogenicity‑like protein
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base T (2/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-01

CGTCAGCCATGCACTCCAACCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCACGGCAATGGC  >  NC_002947/5747314‑5747604
                                                                                                                                                |                                                                                                                                                  
cggcAGCCATGCACTCCACCCGCGTTTCGAGGCAGCGGTGCTGATGAACTGCTCGAGGCGGAGCTCGGGCAACGCGGCAGCCAGGAGGTCCATGCCCCTGCTGGCGCCAGCGGCGATGGGGACAGGCCGCGGCAGCGCCGGCGccgccg                                                                                                                                                >  8:267850/4‑149 (MQ=255)
 gTCAGCCATGCACTACAACCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGc                                                                                                                                               >  3:333010/1‑149 (MQ=255)
            aCTCCAACCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACg                                                                                                                                      >  1:23178/1‑147 (MQ=255)
            aCTCCAACCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCa                                                                                                                                    <  6:363160/149‑1 (MQ=255)
              tCCAACCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGc                                                                                                                                  >  7:148512/1‑149 (MQ=255)
              tCCAACAGCGTTTCGAGGCACAGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGATGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGc                                                                                                                                  >  7:315321/1‑149 (MQ=255)
                 aaCCGCGTTTCGGGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCaa                                                                                                                               >  8:69280/1‑149 (MQ=255)
                 aaCCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCaa                                                                                                                               >  7:349864/1‑149 (MQ=255)
                  aCCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAAt                                                                                                                              >  2:322215/1‑149 (MQ=255)
                        tttCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGcc                                                                                                                         >  6:266211/1‑148 (MQ=255)
                              ggCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTAt                                                                                                                  <  1:192048/149‑1 (MQ=255)
                                cACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATgg                                                                                                                <  2:23178/149‑1 (MQ=255)
                                cACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATgg                                                                                                                <  1:248857/149‑1 (MQ=255)
                                 aCCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATggg                                                                                                               >  3:143759/1‑149 (MQ=255)
                                        atgatgaACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACgg                                                                                                         <  7:228349/148‑1 (MQ=255)
                                         tgatgaACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGTGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGca                                                                                                       <  7:87741/149‑1 (MQ=255)
                                          gatgaACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGcat                                                                                                      >  3:264641/1‑149 (MQ=255)
                                            tgaACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGcatca                                                                                                    >  1:313849/1‑149 (MQ=255)
                                                   cTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTTGCGACATCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTcgc                                                                                             <  4:143759/149‑1 (MQ=255)
                                                   cTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGTCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTcgc                                                                                             <  8:315321/149‑1 (MQ=255)
                                                   cTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTc                                                                                               <  8:287447/147‑1 (MQ=255)
                                                    tCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTcgcg                                                                                            >  4:142575/1‑149 (MQ=255)
                                                    tCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTcgcg                                                                                            >  5:148845/1‑149 (MQ=255)
                                                        ggCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATc                                                                                        <  2:44091/149‑1 (MQ=255)
                                                                     cAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCAt                                                                           >  1:167483/1‑149 (MQ=255)
                                                                      aaCGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCAt                                                                           >  7:215674/1‑148 (MQ=255)
                                                                      aaCGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCAt                                                                           >  7:209969/1‑148 (MQ=255)
                                                                            tCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACgg                                                                      >  4:83093/1‑147 (MQ=255)
                                                                            tCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGaa                                                                    <  4:333010/149‑1 (MQ=255)
                                                                              aGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAgg                                                                  >  1:231211/1‑149 (MQ=255)
                                                                                ccATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGt                                                                 >  6:210359/1‑148 (MQ=255)
                                                                                   tGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTACATCACGGAAGGTcaca                                                             <  8:349864/149‑1 (MQ=255)
                                                                                      ggTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATAc                                                          <  3:83093/149‑1 (MQ=255)
                                                                                       gTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACt                                                         >  3:324713/1‑149 (MQ=255)
                                                                                       gTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACt                                                         >  5:52165/1‑149 (MQ=255)
                                                                                          cATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGt                                                      >  5:174325/1‑149 (MQ=255)
                                                                                            tGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCTCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTg                                                    <  5:196082/149‑1 (MQ=255)
                                                                                            tGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTg                                                    <  2:313849/149‑1 (MQ=255)
                                                                                              ccccTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTgg                                                   <  5:266211/148‑1 (MQ=255)
                                                                                              ccccTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTgg                                                   <  3:142575/148‑1 (MQ=255)
                                                                                               cccTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTggg                                                  >  6:60471/1‑148 (MQ=255)
                                                                                               ccatgctgGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTggg                                                  >  4:262394/4‑148 (MQ=255)
                                                                                                       gCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGc                                          <  5:210359/148‑1 (MQ=255)
                                                                                                       gCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGc                                          <  6:52165/148‑1 (MQ=255)
                                                                                                       gCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCt                                         <  7:267850/149‑1 (MQ=255)
                                                                                                         gACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTg                                        >  2:159623/1‑148 (MQ=255)
                                                                                                              cGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGAt                                  <  6:148845/149‑1 (MQ=255)
                                                                                                              cGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGCGCACATCGCTGATccct                                  >  7:361444/1‑146 (MQ=255)
                                                                                                                gCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGTACATCGCTGATCGATGc                                <  4:264641/149‑1 (MQ=255)
                                                                                                                   aTTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCACAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCca                              >  5:7991/1‑148 (MQ=255)
                                                                                                                    ttGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCcac                             >  7:282991/1‑148 (MQ=255)
                                                                                                                       gCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCgcgc                                                                                           <  4:168326/83‑1 (MQ=255)
                                                                                                                       gCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCgcgc                                                                                           >  3:168326/1‑83 (MQ=255)
                                                                                                                       gCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCacaaca                         <  1:322215/149‑1 (MQ=255)
                                                                                                                          caTGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTc                      <  4:324713/149‑1 (MQ=255)
                                                                                                                              cAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCtgtg                  >  4:207925/1‑149 (MQ=255)
                                                                                                                              cAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCtgt                   <  3:262394/148‑1 (MQ=255)
                                                                                                                               aGCGGCTGCGCCGGAGCTGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTtgt                                                                               >  8:39978/1‑87 (MQ=38)
                                                                                                                               aGCGGCTGCGCCGGAGCTGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTtgt                                                                               <  7:314496/87‑1 (MQ=38)
                                                                                                                               aGCGGCTGCGCCGGAGCTGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTtgt                                                                               >  8:314496/1‑87 (MQ=38)
                                                                                                                               aGCGGCTGCGCCGGAGCTGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTtgt                                                                               <  7:39978/87‑1 (MQ=38)
                                                                                                                               aGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCTGCCAATGCGCCCAGGTTTGGGCCACGGCATCATTCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTg                                                    <  7:247362/114‑1 (MQ=255)
                                                                                                                               aGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTgtgt                 <  2:167483/149‑1 (MQ=255)
                                                                                                                                     aGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCAc           <  2:231211/149‑1 (MQ=255)
                                                                                                                                        tCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCACgg         >  2:11712/1‑148 (MQ=255)
                                                                                                                                          cGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCACGGCaa      >  8:335321/1‑149 (MQ=255)
                                                                                                                                            gcgcAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCACGGCAATg    <  8:282991/149‑1 (MQ=255)
                                                                                                                                              gcAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCACGGCAATGGc  <  3:7234/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGTCAGCCATGCACTCCAACCGCGTTTCGAGGCACCGGTGATGATGAACTGCTCGAGGCGTAGCTCGGGCAACGCGTCAGCCATGAGGTCCATGCCCCTGCTGGCGACAGCGGCCATTGGCACATGCAGCGGCAGCTCCGGCGCAGCCGCTTCGTTACGCAGCCAATGCGCCCAGGTATGGGCCACGGCATCATCCTCGCGCATCGGCGCTTGTGCATCACGGAAGGTCACATACTGGTTGGGCACATCGCTGATCGATGCCACAACAGTCTGTGTCTGCACGGCAATGGC  >  NC_002947/5747314‑5747604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑