| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 445,105 | A→T | 12.8% | intergenic (+78/‑210) | PP_0367 → / → PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 445,105 | 0 | A | T | 12.8% | 119.0 / 9.5 | 53 | intergenic (+78/‑210) | PP_0367/PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base T (2/5); total (25/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
GAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAAC > NC_002947/444959‑445252 | gAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAAtt > 4:189648/1‑149 (MQ=255) gCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAAtttt < 3:189648/149‑1 (MQ=255) tCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTg > 8:251460/1‑148 (MQ=255) tCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAAGAGGCCCGACCAGACAAAATtgttgt > 7:174226/1‑149 (MQ=37) ggCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTc < 7:261096/148‑1 (MQ=255) aaGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCgg < 7:26976/149‑1 (MQ=255) aaGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGACCAGACAAAATTGTTGTCTGATCgg < 3:172705/149‑1 (MQ=38) aGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGACCAGACAAAATTGTTGTCTGATCgga < 5:69949/149‑2 (MQ=38) aGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGACCAGACAAAATTGTTGTCTGATCgga < 4:202749/149‑2 (MQ=38) ggTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc < 3:79293/149‑1 (MQ=255) aaGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTc < 3:366860/148‑1 (MQ=255) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCGTTCCCCCGGAATG‑GTGCGATCAGACAACAAATTTTTGGGGTGGGGGCGCTTcggcg > 4:33868/1‑146 (MQ=255) cATCCGACGACATGCTTGGCCGGATCATCGACTCCTACGCCTGATTACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg < 8:174226/148‑1 (MQ=17) cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCGGGCAAg < 4:177834/149‑1 (MQ=17) acgaTATGCTTGGCAGGATCATCGTCACCTACGCCTGAATACTTGCGTCATGCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCTATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGTCAAGcc < 2:210303/149‑1 (MQ=39) ggCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt < 6:22090/113‑1 (MQ=255) ggCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCt > 5:22090/1‑113 (MQ=255) tcatcGACACCTCCGCCTGAATACTTGCGCCATCCCCTATTTCCGCCCCTGCCTTGTCGGCGCGGGC‑TTGCCCGCTATTGCGCCCGACCACACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGCCAAGCCCGCTCCTACAAGGGTTtg < 2:123212/149‑1 (MQ=255) acacCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATAAGACAACAATTTTTTCTGGTGGGGCCTCTTAGCGGGCCATCCCGCGCCAACAAGGGTTTGttgcat > 5:126923/1‑149 (MQ=255) cTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTc < 7:200494/110‑1 (MQ=255) cTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTc > 8:200494/1‑110 (MQ=255) cGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAAAACCTCCGATAACACAACAAATTTTTATTGTAGGGCCACTTAGCGGGGAAGCCCGCAGCAAAAAAGGTTTGTGgcatggcatg > 1:118506/1‑148 (MQ=255) gCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGAc < 7:251460/149‑1 (MQ=255) gAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGAGAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCaa < 6:225343/110‑1 (MQ=255) gAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCaa > 5:225343/1‑110 (MQ=255) tCCCCTATTCCAGCGCCGCCCTGGGAGGAGCGGGC‑TGCCCCGCGAAGGCGTCCGCGCCTCCAACAATTTTGGCTGGGCGGGCCGCTGCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGt < 6:285565/149‑1 (MQ=255) cccggctgcctTGTAGGAGCGGTG‑AGGCCCGGTAATGGTGCCGATCCGCCAAAAATTTTGTCGGGGCGGGCGTCTTCGCGGGAACGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATg < 4:264385/143‑1 (MQ=255) cgcATGCCTTGTAGGAGCGGGC‑TTGCCCGCTAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCa < 2:118506/149‑1 (MQ=255) ctgcctTGTAGGAGCGGGC‑TTGCCCGCGATTGGGTCCTATCAGACAACAATTTTGTCTGGTGGGGCCTCTTCGCGGGAAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCa < 6:126923/149‑1 (MQ=38) gcattGTAGGAGCGGGC‑TTGCCCGCGAATGCGCCCGATCAGACAACAATTTTGTCGGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATa < 1:88468/146‑1 (MQ=255) cGGGC‑TTCCCCGCTATTGCGCCCGAACACAAAAAAATTTTGTCTGTGCGGCCCTCTTCCCGGGCAACCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa < 8:336129/149‑1 (MQ=255) ggC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCa > 5:19242/1‑130 (MQ=255) ggC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa > 1:176777/1‑147 (MQ=255) ggC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGCCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa > 1:106321/1‑147 (MQ=255) gC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa > 4:348018/1‑146 (MQ=255) cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATc < 1:363410/136‑1 (MQ=255) cccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATc > 2:363410/1‑136 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTTCAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATgg < 5:66801/121‑1 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTTCAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATgg > 6:66801/1‑121 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGAc < 6:363920/92‑1 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGAc > 5:363920/1‑92 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATa < 4:360991/128‑1 (MQ=255) ccGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATa > 3:360991/1‑128 (MQ=255) gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCt > 6:282056/1‑147 (MQ=255) gcgAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa < 3:348018/149‑1 (MQ=255) gAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGc > 2:338758/1‑149 (MQ=255) tCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACg > 2:92127/1‑148 (MQ=255) ccGACCAGACAAAATTGTTGTCTGATCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGt > 1:352383/1‑148 (MQ=38) tCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGtt > 3:384929/1‑149 (MQ=255) aGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa < 3:323484/133‑1 (MQ=255) aGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGa > 4:323484/1‑133 (MQ=255) acaacaATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACGATAGTCCATCCTAGACAGAAAGCTGAGCTAAAGGTAAGTTTCa > 8:66768/1‑148 (MQ=255) acaacaATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTATTCGTGGCGTAAATGGGACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCa > 6:298243/1‑148 (MQ=255) caacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg < 6:29701/131‑1 (MQ=255) caacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAg > 5:29701/1‑131 (MQ=255) aacaaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCaaa > 3:36887/1‑148 (MQ=255) aaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaac > 6:343515/1‑149 (MQ=255) aaTTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAaacaac > 6:262613/1‑149 (MQ=255) | GAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGC‑TTGCCCGCGAATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAACGTAAGTTTCAAACAAC > NC_002947/444959‑445252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |