Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,825,240 G→A 6.5% S78F (TCC→TTC)  trmB ← tRNA (guanosine(46)‑N7)‑methyltransferase TrmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,825,2400GA 6.5% 276.5 / 4.5 91S78F (TCC→TTC) trmBtRNA (guanosine(46)‑N7)‑methyltransferase TrmB
Reads supporting (aligned to +/- strand):  ref base G (45/40);  new base A (4/2);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATG  >  NC_002947/5825095‑5825383
                                                                                                                                                 |                                                                                                                                               
cACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAAt                                                                                                                                              <  5:311982/149‑1 (MQ=255)
   ttCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGATCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGc                                                                                                                                           >  6:374545/1‑149 (MQ=255)
     cGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGc                                                                                                                                           <  8:40376/147‑1 (MQ=255)
       aTGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCAt                                                                                                                                       <  6:375759/149‑1 (MQ=255)
           cATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTGGAAATGGccgct                                                                                                                                       <  1:144459/145‑4 (MQ=255)
           cATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTGGAAATGGccgct                                                                                                                                       >  2:144459/1‑142 (MQ=255)
           cATCGCAGTCATACACCCGCAGGTTTATCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGcc                                                                                                                                    >  1:9753/1‑148 (MQ=255)
               gCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCATGGGCCATTCCCTGCTGGAAATGGCCGCTGCCgcac                                                                                                                                <  6:125743/148‑4 (MQ=38)
               gCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAg                                                                                                                                <  3:188245/148‑1 (MQ=255)
               gCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGc                                                                                                                               <  3:251185/149‑1 (MQ=255)
                 aGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGcc                                                                                                                              >  5:333103/1‑148 (MQ=255)
                       acacCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGAtctc                                                                                                                        >  4:235218/1‑148 (MQ=255)
                        cacCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTcc                                                                                                                       <  6:323021/148‑1 (MQ=255)
                         acCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCaa                                                                                                                     <  2:293579/149‑1 (MQ=255)
                          cccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAg                                                                                                                    <  5:48994/149‑1 (MQ=255)
                          cccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAg                                                                                                                    <  2:363742/149‑1 (MQ=255)
                           ccGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAgg                                                                                                                   >  6:281052/1‑149 (MQ=255)
                            cGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGt                                                                                                                  >  6:378491/1‑149 (MQ=255)
                             gCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTg                                                                                                                 <  2:9753/149‑1 (MQ=255)
                               aGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCATCACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTgcg                                                                                                               >  1:279763/1‑149 (MQ=255)
                                  tttttCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCgg                                                                                                            >  3:263240/1‑149 (MQ=255)
                                     ttCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCgggg                                                                                                          <  3:372929/148‑1 (MQ=255)
                                      tCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggc                                                                                                         <  7:226915/148‑1 (MQ=255)
                                       cAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggcc                                                                                                        <  5:378491/148‑1 (MQ=255)
                                          ccccTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaac                                                                                                    >  5:128353/1‑149 (MQ=255)
                                            ccTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCTGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGGGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaacgg                                                                                                  <  6:1640/149‑1 (MQ=255)
                                            ccTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaacgg                                                                                                  >  8:345417/1‑149 (MQ=255)
                                             cTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGTGGggccgaacggc                                                                                                 <  3:235218/149‑1 (MQ=255)
                                             cTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGggccgaacggc                                                                                                 <  5:281052/149‑1 (MQ=255)
                                                   cAGCACGCCA‑TGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAacac                                                                                          >  4:125707/1‑149 (MQ=255)
                                                    aGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAacac                                                                                          <  4:263240/149‑1 (MQ=255)
                                                        cGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTgg                                                                                      >  6:295249/1‑149 (MQ=255)
                                                        cGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTgg                                                                                      <  2:225471/149‑1 (MQ=255)
                                                        cGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTgg                                                                                      >  2:380791/1‑149 (MQ=255)
                                                         gCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTACACACAGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCAAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCAGAACGGCCGAACACCTGGt                                                                                     >  1:372483/1‑149 (MQ=255)
                                                                agtagcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTc                                                                              >  8:131044/4‑149 (MQ=255)
                                                                agcagcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCTGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGACCGGCCGAACCCCTGGTCGTAGTc                                                                              >  8:212187/1‑149 (MQ=255)
                                                                agcagcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTc                                                                              >  6:220954/1‑149 (MQ=255)
                                                                 gcagcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTTCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTc                                                                              >  6:157542/1‑148 (MQ=255)
                                                                 gcagcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTc                                                                              >  6:112645/1‑148 (MQ=255)
                                                                  cagcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCa                                                                            >  4:369553/1‑149 (MQ=255)
                                                                   agcGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCAc                                                                           >  5:304499/1‑149 (MQ=255)
                                                                     cgcgcCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCg                                                                         >  7:43375/1‑149 (MQ=255)
                                                                      gcgcCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCgg                                                                        >  3:213183/1‑149 (MQ=255)
                                                                       cgcCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCggg                                                                       <  2:341571/149‑1 (MQ=255)
                                                                        gcCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGc                                                                      >  8:133539/1‑149 (MQ=255)
                                                                            acacCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATc                                                                  <  7:317394/149‑1 (MQ=255)
                                                                            acacCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATc                                                                  <  3:125707/149‑1 (MQ=255)
                                                                             cacCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCa                                                                 <  2:279763/149‑1 (MQ=255)
                                                                              acCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAg                                                                <  8:43375/149‑1 (MQ=255)
                                                                              acCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAg                                                                >  2:380163/1‑149 (MQ=255)
                                                                               cccGGGCGGTGCACTTCAACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGTTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTAtgatc                                                               <  2:372483/149‑5 (MQ=255)
                                                                                ccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                               >  8:272580/1‑148 (MQ=255)
                                                                                ccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                               >  3:282947/1‑148 (MQ=255)
                                                                                ccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                               >  5:12363/1‑148 (MQ=255)
                                                                                ccGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                               >  7:97577/1‑148 (MQ=255)
                                                                                ccGGGCGGTGCACTTCCACACCGAAGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGCTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                               >  6:211599/1‑148 (MQ=255)
                                                                                 cGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGc                                                               >  5:278403/1‑147 (MQ=255)
                                                                                      ggTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGc                                                        <  5:116646/149‑1 (MQ=255)
                                                                                        tGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAg                                                      >  5:115838/1‑149 (MQ=255)
                                                                                           aCTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGAt                                                   <  7:345417/149‑1 (MQ=255)
                                                                                                     cGATGAAATCCTGCTCAGGTGCGGCAGCCGCCATTTCCAGCAGGGAAAGGACCAAGCCGAAGCCGATCTCCAAGGTGCGAGGGGCCGAACGGCCGAACACCTGCACGAAGTCAACCCGGCTAACAGCCAGTGGCAGGATGTACAGCgtg                                         >  7:40441/1‑147 (MQ=255)
                                                                                                       aTGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGgcc                                       >  7:16812/1‑149 (MQ=255)
                                                                                                        tGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGgccg                                      >  1:271825/1‑149 (MQ=255)
                                                                                                           aaTCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGgccgcc                                    <  8:97577/148‑1 (MQ=255)
                                                                                                            aTCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCtt                                  >  5:312663/1‑149 (MQ=255)
                                                                                                             tCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTg                                 <  6:12363/149‑1 (MQ=255)
                                                                                                               cTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTTCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTgg                                <  5:157542/148‑1 (MQ=255)
                                                                                                                   tCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGGGGCAGGATGTACCGCGGGCCGCCGTgggcgag                           >  3:295316/1‑144 (MQ=255)
                                                                                                                     aGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCAAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGAc                         >  6:274399/1‑149 (MQ=255)
                                                                                                                        ttcGGCATGGGCCATTCCCTGCTGGAAATGGCCGCTGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACcgc                      >  8:161277/3‑149 (MQ=38)
                                                                                                                        tGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACcgc                      >  2:379597/1‑149 (MQ=255)
                                                                                                                           ggCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGtt                   <  8:16812/149‑1 (MQ=255)
                                                                                                                             catgggCCATTCCCTGCTGGAAATGGCCCATGCCGAAGCCGATCTCCAAGGTGAGCGGGGCCGAACGCCCTAACACCTGGTCGTAGTCCACCGGGCTATCAGACAGTGGCAGGATGTACAGCGGGCCCCCTTGATCCAGACCGCGATg                  >  8:78477/5‑148 (MQ=255)
                                                                                                                               tgggCCATTCCCTGCTGGAAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGCACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTg               >  1:156261/3‑149 (MQ=255)
                                                                                                                               gCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTg               <  4:282947/149‑1 (MQ=255)
                                                                                                                               gCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTg               <  2:271825/149‑1 (MQ=255)
                                                                                                                                  gCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGcc            <  6:304499/149‑1 (MQ=255)
                                                                                                                                   ccATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCt           >  7:296632/1‑149 (MQ=255)
                                                                                                                                   ccATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTAGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCt           >  6:62240/1‑149 (MQ=255)
                                                                                                                                    cATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCtt          >  3:213269/1‑149 (MQ=255)
                                                                                                                                     aTTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTc         >  5:244414/1‑149 (MQ=255)
                                                                                                                                      tttCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCg        <  6:312663/149‑1 (MQ=255)
                                                                                                                                      tttCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCg        <  4:213183/149‑1 (MQ=255)
                                                                                                                                        tCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCgg       <  5:295249/148‑1 (MQ=255)
                                                                                                                                        tCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCgg       <  1:380791/148‑1 (MQ=255)
                                                                                                                                          cagcagGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGTCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGTCCTTCGGTc     <  3:369553/148‑1 (MQ=255)
                                                                                                                                          cagcagGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGtt                   >  4:357206/1‑134 (MQ=255)
                                                                                                                                          cagcagGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGtt                   <  3:357206/134‑1 (MQ=255)
                                                                                                                                          cagcagGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCa    >  7:17975/1‑149 (MQ=255)
                                                                                                                                            gcagGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATg  <  6:115838/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CACTTCGATGGCATCGCAGTCATACACCCGCAGGTTTTTCAGCCCCTGGGTCAGCACGCCATTGAGCAGCGCGCCAACACCCGGGCGGTGCACTTCCACACCGATGAAATCCTGCTCAGGTGCGGCAGCGGCCATTTCCAGCAGGGAATGGCCCATGCCGAAGCCGATCTCCAAGGTGCGCGGGGCCGAACGGCCGAACACCTGGTCGTAGTCCACCGGGCTATCAGCCAGTGGCAGGATGTACAGCGGGCCGCCTTGGTCCAGACCGCGTTGCTGGCCTTCGGTCATG  >  NC_002947/5825095‑5825383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑