Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,855,103 C→A 5.9% L375M (CTG→ATG) ‡ tcyP → sodium/cystine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,855,1030CA 5.9% 280.2 / 4.7 85L375M (CTG→ATG) ‡tcyPsodium/cystine symporter
Reads supporting (aligned to +/- strand):  ref base C (44/36);  new base A (2/3);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GCAATCGATCGCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGATCGACATGGG  >  NC_002947/5854961‑5855251
                                                                                                                                              |                                                                                                                                                    
gCAATCGATCGCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCtggtgg                                                                                                                                                <  1:346186/149‑1 (MQ=255)
gCAATCGATCGCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCtggtgg                                                                                                                                                >  2:346186/1‑149 (MQ=255)
  aaTCGATCGCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGc                                                                                                                                               >  8:301549/1‑148 (MQ=255)
   atcgatcgCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCAGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCAGGGTGGcga                                                                                                                                             <  2:158973/149‑3 (MQ=255)
       atcgCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCAGGGTGGcg                                                                                                                                              >  4:302191/1‑143 (MQ=255)
       atcgCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCAGGGTGGcg                                                                                                                                              <  3:302191/144‑2 (MQ=255)
        tcgCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTc                                                                                                                                        <  3:221431/149‑1 (MQ=255)
           ccAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACg                                                                                                                                     <  7:314773/149‑1 (MQ=255)
               cTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGa                                                                                                                                 <  3:123614/149‑1 (MQ=255)
                  cAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATGGCCACCAGGGTGGCGATc                                                                                                                                           >  6:309012/1‑136 (MQ=255)
                  cAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATGGCCACCAGGGTGGCGATc                                                                                                                                           <  5:309012/136‑1 (MQ=255)
                       cATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCa                                                                                                                         <  7:301549/149‑1 (MQ=255)
                        aTCGTTCGGGACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAg                                                                                                                        <  8:89773/149‑1 (MQ=255)
                        aTCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAg                                                                                                                        >  3:162936/1‑149 (MQ=255)
                        aTCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAg                                                                                                                        >  3:149415/1‑149 (MQ=255)
                              cGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGtt                                                                                                                   <  3:286080/148‑1 (MQ=255)
                               ggCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTg                                                                                                                  <  6:344249/148‑1 (MQ=255)
                                 caccacCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCg                                                                                                               <  3:286443/149‑1 (MQ=255)
                                  accaccATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAgggg                                                                                                                     <  5:86214/142‑1 (MQ=255)
                                  accaccATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAgggg                                                                                                                     >  6:86214/1‑142 (MQ=255)
                                    caccaTTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCg                                                                                                            >  7:12771/1‑149 (MQ=255)
                                     accaTTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGt                                                                                                           <  4:149415/149‑1 (MQ=255)
                                     accaTTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGt                                                                                                           <  4:162936/149‑1 (MQ=255)
                                     accaTTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGt                                                                                                           >  8:351782/1‑149 (MQ=255)
                                        aTTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTggg                                                                                                        >  2:4628/1‑149 (MQ=255)
                                         ttGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGggc                                                                                                       >  4:187588/1‑149 (MQ=255)
                                            gCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTg                                                                                                                           >  4:38302/1‑126 (MQ=255)
                                            gCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTg                                                                                                                           <  3:38302/126‑1 (MQ=255)
                                              cAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGcggcgg                                                                                                  >  4:182023/1‑149 (MQ=255)
                                               aGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGt                                                                                                 <  4:149567/149‑1 (MQ=255)
                                                         cgcCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCttt                                                                                        <  6:71836/148‑1 (MQ=255)
                                                           ccGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGcc                                                                                     >  8:168921/1‑149 (MQ=255)
                                                               gCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCgcgc                                                                                 <  8:275111/149‑1 (MQ=255)
                                                                ctctATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGt                                                                                                 >  3:332241/1‑132 (MQ=255)
                                                                ctctATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGt                                                                                                 <  4:332241/132‑1 (MQ=255)
                                                                  ctATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGa                                                                              >  5:341025/1‑149 (MQ=255)
                                                                   tATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGAt                                                                             >  8:163099/1‑149 (MQ=255)
                                                                   tATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGAt                                                                             >  6:393974/1‑149 (MQ=255)
                                                                     tCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATc                                                                            <  3:187588/148‑1 (MQ=255)
                                                                     tCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCg                                                                           >  2:108926/1‑149 (MQ=255)
                                                                        ggCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGtgc                                                                        >  5:94481/1‑149 (MQ=255)
                                                                        ggCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGt                                                                          <  7:310484/147‑1 (MQ=255)
                                                                        ggCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGt                                                                          >  8:310484/1‑147 (MQ=255)
                                                                         gCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGtgct                                                                       <  3:380288/149‑1 (MQ=255)
                                                                             tGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTgcca                                                                   <  8:12771/149‑1 (MQ=255)
                                                                               cTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTgccag                                                                  <  6:341025/148‑1 (MQ=255)
                                                                                  gCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCAt                                                              >  3:318782/1‑149 (MQ=255)
                                                                                  gCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCAt                                                              <  7:163099/149‑1 (MQ=255)
                                                                                    ggTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATgg                                                            <  6:94481/149‑1 (MQ=255)
                                                                                    ggTGATGCTCGCGACAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCAATgg                                                            >  6:202056/1‑149 (MQ=255)
                                                                                     gTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATggg                                                           >  3:293383/1‑149 (MQ=255)
                                                                                             cgcgCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTCCCGGCGTGCGCGGCGCTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGACATCGGCTTGcccg                                                   >  8:117893/1‑147 (MQ=255)
                                                                                              gcgcCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCgg                                                   <  5:277724/148‑1 (MQ=255)
                                                                                              gcgcCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGt                                                  >  4:41971/1‑149 (MQ=255)
                                                                                               cgcCAGCAGTAGGCATCGACACCTTCGACCCGGTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTg                                                 <  4:318782/149‑1 (MQ=255)
                                                                                               cgcCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTg                                                 <  4:382202/149‑1 (MQ=255)
                                                                                                gcCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTg                                                 >  5:35927/1‑148 (MQ=255)
                                                                                                   agcagTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAAc                                             >  7:108779/1‑149 (MQ=255)
                                                                                                   agcagTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAAc                                             >  3:24760/1‑149 (MQ=255)
                                                                                                    gcagTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACt                                            <  1:108926/149‑1 (MQ=255)
                                                                                                        tAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACtggtg                                        >  5:107028/1‑149 (MQ=255)
                                                                                                         aGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACtggtgg                                       >  4:382894/1‑149 (MQ=255)
                                                                                                             aTCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGtt                                   >  3:353302/1‑149 (MQ=255)
                                                                                                             aTCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGtt                                   >  2:211035/1‑149 (MQ=255)
                                                                                                                gACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGc                                 <  7:168921/148‑1 (MQ=255)
                                                                                                                 acacCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTg                               <  1:4628/149‑1 (MQ=255)
                                                                                                                  cacCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTACCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGa                              >  4:145762/1‑149 (MQ=255)
                                                                                                                   acCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTCCCGGTGGAACTGGTGGCGTTGCTGag                             >  8:288516/1‑148 (MQ=255)
                                                                                                                    ccTTCGACCCGCTGGGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGAtt                            >  7:334037/1‑149 (MQ=255)
                                                                                                                     cTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTgccag                                                                  >  4:114191/1‑110 (MQ=255)
                                                                                                                     cTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGAttt                           >  7:106518/1‑149 (MQ=255)
                                                                                                                     cTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGACACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGAttt                           >  3:74667/1‑149 (MQ=255)
                                                                                                                      ttCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCCCCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTc                          >  3:199853/1‑149 (MQ=255)
                                                                                                                      atCGACCCGCTGTGGATCGCCACACTGGTGGCCATCGTCACGCTGAGCTCTCCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGAACGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTc                          >  6:91968/2‑149 (MQ=255)
                                                                                                                        cGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCg                         <  4:255011/148‑1 (MQ=255)
                                                                                                                        cGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCg                         <  8:317258/148‑1 (MQ=255)
                                                                                                                         gACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCgg                        <  3:382894/148‑1 (MQ=255)
                                                                                                                         gACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTAGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGt                       >  7:292861/1‑149 (MQ=255)
                                                                                                                            ccGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGa                    <  6:35927/149‑1 (MQ=255)
                                                                                                                                    gATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGa            <  4:293383/149‑1 (MQ=255)
                                                                                                                                    gATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGa            >  4:67646/1‑149 (MQ=255)
                                                                                                                                      tCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGATc          >  8:252823/1‑149 (MQ=255)
                                                                                                                                           aCCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGc               >  2:367376/1‑139 (MQ=255)
                                                                                                                                           aCCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGc               <  1:367376/139‑1 (MQ=255)
                                                                                                                                           aCCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGATCGACAt     >  6:380981/1‑149 (MQ=255)
                                                                                                                                              cTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGATCGACATggg  <  7:38883/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GCAATCGATCGCCAGCTTCAGCGCATCGTTCGGCACCACCATTGGCCAGAACGGCTGCGCCGGGCTCTATCCGGCCATGCTGGCGGTGATGGTCGCGCCAGCAGTAGGCATCGACACCTTCGACCCGCTGTGGATCGCCACCCTGGTGGCCATCGTCACGCTGAGCTCTGCAGGGGTTGCCGGCGTGGGCGGCGGTGCCACCTTTGCCGCGCTGATCGTGCTGCCAGCCATGGGCTTGCCGGTGGAACTGGTGGCGTTGCTGATTTCGGTGGAGCCGCTGATCGACATGGG  >  NC_002947/5854961‑5855251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑