Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,855,824 C→T 6.4% V66M (GTG→ATG)  folA ← dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,855,8240CT 6.4% 231.9 / 4.0 78V66M (GTG→ATG) folAdihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (34/39);  new base T (2/3);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.16e-01

GCCTGCCCGTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCACGCG  >  NC_002947/5855676‑5855971
                                                                                                                                                    |                                                                                                                                                   
gcCTGCCCGTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCAc                                                                                                                                                     <  3:237177/149‑1 (MQ=255)
  cTGCCCGTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGa                                                                                                                                                   <  7:373308/149‑1 (MQ=255)
   tGCCCGTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGAt                                                                                                                                                  >  3:22421/1‑149 (MQ=255)
     cccGTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGAt                                                                                                                                                  <  6:108396/147‑1 (MQ=255)
        gTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGCCCACGATGtt                                                                                                                                               >  5:155951/1‑147 (MQ=255)
           cAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGTCTGGCGGCTGACCACGATGTTCAgcc                                                                                                                                          <  8:325731/149‑1 (MQ=255)
           cAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAgcc                                                                                                                                          <  7:359498/149‑1 (MQ=255)
           cAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAgcc                                                                                                                                          <  1:200069/149‑1 (MQ=255)
             gCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAgccgc                                                                                                                                        <  8:218341/149‑1 (MQ=255)
             gCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAgccgc                                                                                                                                        >  4:268282/1‑149 (MQ=255)
             gCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGAACATCGTGGTCAgccgc                                                                                                                                        <  8:356523/149‑1 (MQ=255)
               tGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGACTGACCACGATGTTCAgccgcc                                                                                                                                       >  7:102028/1‑148 (MQ=255)
                  gcgcCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAgg                                                                                                                                   <  1:129021/149‑1 (MQ=255)
                  gcgcCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAgg                                                                                                                                   <  6:166913/149‑1 (MQ=255)
                  gcgcCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAgg                                                                                                                                   <  4:334407/149‑1 (MQ=255)
                      ccccAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGc                                                                                                                               >  3:164607/1‑149 (MQ=255)
                        ccAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGAACATCGTGGTCAgccgc                                                                                                                                        >  5:248197/1‑138 (MQ=255)
                        ccAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGAACATCGTGGTCAgccgc                                                                                                                                        <  6:248197/138‑1 (MQ=255)
                            tcagcatcagcTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCACGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGgccgc                                                                                                                         >  3:68556/1‑149 (MQ=255)
                                catcagcTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCCGCCGCCCAGGCAGCGGCCGccc                                                                                                                       >  6:212781/1‑147 (MQ=255)
                                 atcagcTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAg                                                                                                                    >  4:226623/1‑149 (MQ=255)
                                     gcTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAg                                                                                                                <  6:126754/149‑1 (MQ=255)
                                     gcTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAg                                                                                                                >  4:342460/1‑149 (MQ=255)
                                         aCCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTcc                                                                                                             <  3:226623/148‑1 (MQ=255)
                                           cGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTCGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAg                                                                                                          >  2:302896/1‑149 (MQ=255)
                                                cccTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGa                                                                                                                 >  7:243099/1‑137 (MQ=255)
                                                cccTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGa                                                                                                                 <  8:243099/137‑1 (MQ=255)
                                                     cTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGcc                                                                                                >  8:352288/1‑149 (MQ=255)
                                                           cgcCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCatga                                                                                          <  7:37787/149‑1 (MQ=255)
                                                           cgcCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCatga                                                                                          <  4:22421/149‑1 (MQ=255)
                                                              ccACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCatgatg                                                                                        >  7:324416/1‑148 (MQ=255)
                                                              ccACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCAtgatga                                                                                       >  5:216751/1‑149 (MQ=255)
                                                               cACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATgatgat                                                                                      >  2:77802/1‑149 (MQ=255)
                                                                    cTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCt                                                                                 >  6:2541/1‑149 (MQ=255)
                                                                         cccGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGcc                                                                             >  1:110590/1‑148 (MQ=255)
                                                                          ccGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTCGCGCCCCATGAGGATCGGCTTGcc                                                                             >  4:77607/1‑147 (MQ=255)
                                                                            gCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAgg                                                                         <  2:306659/149‑1 (MQ=255)
                                                                              aCCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGggt                                                                       >  1:172737/1‑149 (MQ=255)
                                                                                cAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTgc                                                                                                   >  6:4338/1‑119 (MQ=255)
                                                                                cAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTgc                                                                                                   <  5:4338/119‑1 (MQ=255)
                                                                                cAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGggtgg                                                                     >  7:334222/1‑149 (MQ=255)
                                                                                  aCGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGgtggtg                                                                   <  2:110590/149‑1 (MQ=255)
                                                                                  aCGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGgtggtg                                                                   <  3:80493/149‑1 (MQ=255)
                                                                                     ccTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGcc                                                                <  5:2541/149‑1 (MQ=255)
                                                                                       tctTAAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACCATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGcc                                                                >  4:353532/1‑147 (MQ=255)
                                                                                         ttCAAGCGAGGCGAACACTTCGGCACCTTCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGACGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCtt                                                              <  4:383602/147‑1 (MQ=255)
                                                                                         ttCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCtt                                                              <  1:302896/147‑1 (MQ=255)
                                                                                         ttCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTg                                                             >  1:82113/1‑148 (MQ=255)
                                                                                          acAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCACGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGCCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCACGGTGGTGGCCTTg                                                             >  1:340695/2‑147 (MQ=255)
                                                                                           cAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGaa                                                           <  4:164607/148‑1 (MQ=255)
                                                                                           cAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGaa                                                           <  6:216751/148‑1 (MQ=255)
                                                                                                gAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGGCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACtt                                                     <  8:334222/149‑1 (MQ=255)
                                                                                                gAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACtt                                                     <  3:268282/149‑1 (MQ=255)
                                                                                                gAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACtt                                                     <  3:145998/149‑1 (MQ=255)
                                                                                                gAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACtt                                                     <  7:352288/149‑1 (MQ=255)
                                                                                                   gCGAACACTTCTGCACCTGCAAGTTCAAGCCCGGGCTGGCGGCTGACCACTATGTTCAGCCGCCCAGGCATCGGCCGCCCCAGCGAGTCCCAGGTCATGCGCCCCATGATGTTCGGCTTGCCCAGGGTGGTGGCCTTGAATTACTTGaa                                                  <  2:131591/149‑1 (MQ=255)
                                                                                                    cGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAg                                                 <  1:77802/149‑1 (MQ=255)
                                                                                                     gAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACtt                                                     >  7:50063/1‑144 (MQ=255)
                                                                                                     gAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACtt                                                     <  8:50063/144‑1 (MQ=255)
                                                                                                        cacTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTccc                                             <  5:267871/149‑1 (MQ=255)
                                                                                                           ttCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCg                                          >  8:173715/1‑149 (MQ=255)
                                                                                                               gCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTg                                                             >  7:172044/1‑126 (MQ=255)
                                                                                                               gCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTg                                                             <  8:172044/126‑1 (MQ=255)
                                                                                                                     gCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGc                                  >  1:146382/1‑147 (MQ=255)
                                                                                                                       aaGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAgg                              <  2:172737/149‑1 (MQ=255)
                                                                                                                         gTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAggg                             >  4:112498/1‑148 (MQ=255)
                                                                                                                          ttCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCa                           >  8:197587/1‑149 (MQ=255)
                                                                                                                          ttCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCa                           >  7:126090/1‑149 (MQ=255)
                                                                                                                              aGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGa                       <  8:5308/149‑1 (MQ=255)
                                                                                                                                    ggCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTc                 <  1:273050/149‑1 (MQ=255)
                                                                                                                                        ggcggcTGAACATCGTGGTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGAt              <  1:359637/148‑1 (MQ=255)
                                                                                                                                        ggcggcTGAACATCGTGGTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGAt              >  2:359637/1‑148 (MQ=255)
                                                                                                                                         gcggcTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGc            <  3:112498/149‑1 (MQ=255)
                                                                                                                                          cggcTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGcc           <  2:324820/149‑1 (MQ=255)
                                                                                                                                                  cACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCAcgc   >  3:42771/1‑149 (MQ=255)
                                                                                                                                                  cACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCAcgc   >  3:76128/1‑149 (MQ=255)
                                                                                                                                                  cACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGGGGCCTTGAAGTACTTCAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCAcgc   <  8:324416/149‑1 (MQ=255)
                                                                                                                                                   aCGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCAcgcg  >  3:229258/1‑149 (MQ=255)
                                                                                                                                                   aCGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCAcgcg  <  5:212781/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCTGCCCGTACAGCTGCGCGCCCCCAATCAGCATCAGCTCACCGACGCCCTGCTCGCGCGCCCACTGCTCGGCCCGCACCAACGCCTCTTCAAGCGAGGCGAACACTTCGGCACCTGCAAGTTCAAGCCCCGGCTGGCGGCTGACCACGATGTTCAGCCGCCCAGGCAGCGGCCGCCCCAGCGAGTCCCAGGTCTTGCGCCCCATGATGATCGGCTTGCCCAGGGTGGTGGCCTTGAAGTACTTGAAGTCCCCCGGCAAATGCCAGGGCATGGAGTTGTCGATGCCGATCACGCG  >  NC_002947/5855676‑5855971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑