Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,857,965 C→G 5.1% R172G (CGG→GGG)  PP_5134 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,857,9650CG 5.1% 246.8 / 3.8 78R172G (CGG→GGG) PP_5134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (36/38);  new base G (2/2);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

TGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGTTT  >  NC_002947/5857822‑5858113
                                                                                                                                               |                                                                                                                                                    
tgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCtt                                                                                                                                                 <  2:110307/149‑1 (MQ=255)
 gATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCCGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                                >  8:218562/1‑149 (MQ=255)
  atagATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTTGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGAACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                                <  5:231811/145‑1 (MQ=255)
  aTCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                                <  6:114159/148‑1 (MQ=255)
  aTCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                                <  5:230704/148‑1 (MQ=255)
   tCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTggg                                                                                                                                              >  1:25351/1‑149 (MQ=255)
    cGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCATGGGc                                                                                                                                             >  2:111545/1‑149 (MQ=255)
      aTGCGCGCTAGGCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCACCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCtt                                                                                                                                           <  3:13169/149‑1 (MQ=255)
        gcgcgcGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCATCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTc                                                                                                                                          <  5:25218/148‑1 (MQ=255)
         cgcgcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCAc                                                                                                                                        <  4:68033/149‑1 (MQ=255)
         cgcgcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCAc                                                                                                                                        <  6:3036/149‑1 (MQ=255)
            gcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCa                                                                                                                                     >  2:77026/1‑149 (MQ=255)
            gcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCa                                                                                                                                     >  5:115807/1‑149 (MQ=255)
            gcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCTTCa                                                                                                                                         <  8:48110/145‑1 (MQ=255)
            gcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCTTCa                                                                                                                                         >  7:48110/1‑145 (MQ=255)
               agagggTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAt                                                                                                                                    <  1:337811/143‑1 (MQ=255)
               aGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTg                                                                                                                                  <  5:108030/149‑1 (MQ=255)
                  cGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTa                                                                                                                               >  3:68401/1‑149 (MQ=255)
                          gCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGa                                                                                                                       <  8:35470/149‑1 (MQ=255)
                            cAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGa                                                                                                                       >  5:72586/1‑147 (MQ=255)
                            cAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATCGGTACGGTTCGACa                                                                                                                     <  7:334182/149‑1 (MQ=255)
                            cAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGcaa                                                                                                                                                 <  3:174008/121‑3 (MQ=255)
                            cAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGcaa                                                                                                                                                 >  4:174008/1‑119 (MQ=255)
                             aaGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAg                                                                                                                    >  7:206080/1‑149 (MQ=255)
                                tACCGCGACTAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCGCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGt                                                                                                                 <  2:218499/149‑1 (MQ=255)
                                  ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGACGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTgg                                                                                                               <  5:102416/149‑1 (MQ=255)
                                         gaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                         <  7:180765/148‑1 (MQ=255)
                                         gaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                         >  8:180765/1‑148 (MQ=255)
                                           aCTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                         >  5:36795/1‑146 (MQ=255)
                                               gAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGa                                                                                                  >  1:263096/1‑149 (MQ=255)
                                                aaGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGAt                                                                                                 <  8:119459/149‑1 (MQ=255)
                                                 aGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATg                                                                                                <  1:77026/149‑1 (MQ=255)
                                                   tGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                              <  1:195362/149‑1 (MQ=255)
                                                   tGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                              <  6:115807/149‑1 (MQ=255)
                                                    gCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                              >  8:18182/1‑148 (MQ=255)
                                                       ggCAAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCCAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTAGACAGCGTGGCCCCGCCAGAGGATGGTGAgc                                                                                          >  4:72499/1‑149 (MQ=255)
                                                        gCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAgc                                                                                          <  1:228930/148‑1 (MQ=255)
                                                            aCTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGt                                                                                     >  6:35376/1‑149 (MQ=255)
                                                             cTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGtt                                                                                    >  5:169503/1‑149 (MQ=255)
                                                               gCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAgg                                                                                                   >  3:390018/1‑132 (MQ=255)
                                                               gCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAgg                                                                                                   <  4:390018/132‑1 (MQ=255)
                                                                cGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGttt                                                                                   <  4:122057/147‑1 (MQ=255)
                                                                cGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGACCCCGCCAGAGGATGGTGAGCGCCGTATg                                                                                  >  2:165143/1‑148 (MQ=255)
                                                                 ggCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTa                                                                                <  2:25351/149‑1 (MQ=255)
                                                                   cAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTAc                                                                               >  1:386547/1‑148 (MQ=255)
                                                                      gCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGaaa                                                                           <  7:18182/149‑1 (MQ=255)
                                                                             ctgcCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgc                                                                    <  1:154147/149‑1 (MQ=255)
                                                                              tgcCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgcg                                                                   >  7:19264/1‑149 (MQ=255)
                                                                                ccGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgct                                                                 >  7:71323/1‑149 (MQ=255)
                                                                                ccGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgct                                                                 <  2:263096/149‑1 (MQ=255)
                                                                                 cGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctg                                                                >  6:29261/1‑149 (MQ=255)
                                                                                     gCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctgctgc                                                            >  3:38875/1‑149 (MQ=255)
                                                                                       tCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGt                                                                                 >  2:178780/1‑126 (MQ=255)
                                                                                       tCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGt                                                                                 <  1:178780/126‑1 (MQ=255)
                                                                                        cAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTgg                                                         <  4:68401/149‑1 (MQ=255)
                                                                                              cgtcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACTGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAc                                                    <  6:72586/148‑1 (MQ=255)
                                                                                                tcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgcc                                                 >  8:150023/1‑149 (MQ=255)
                                                                                                    cagcagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgcccgcc                                             <  2:259441/149‑1 (MQ=255)
                                                                                                    cagcagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgcccgcc                                             >  3:370872/1‑149 (MQ=255)
                                                                                                        agccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGgc                                         <  6:36795/149‑1 (MQ=255)
                                                                                                         gccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGgcg                                        >  1:293557/1‑149 (MQ=255)
                                                                                                                 cgcgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACg                                >  3:377281/1‑149 (MQ=255)
                                                                                                                   cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                              <  8:71323/149‑1 (MQ=255)
                                                                                                                   cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                              <  4:377281/149‑1 (MQ=255)
                                                                                                                   cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                              <  4:370872/149‑1 (MQ=255)
                                                                                                                    gAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCt                             <  8:206080/149‑1 (MQ=255)
                                                                                                                       ccTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAGCGGCTGAt                          >  4:281186/1‑149 (MQ=255)
                                                                                                                       ccTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGAt                          >  4:6786/1‑149 (MQ=255)
                                                                                                                        cTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATc                         >  5:312915/1‑149 (MQ=255)
                                                                                                                         tGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcg                        >  5:181327/1‑149 (MQ=255)
                                                                                                                           aaTGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcgac                      <  5:35376/149‑1 (MQ=255)
                                                                                                                             tGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATcgacga                    <  1:111545/149‑1 (MQ=255)
                                                                                                                                 gTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACcag                >  4:176745/1‑149 (MQ=255)
                                                                                                                                 gTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACcag                >  6:397888/1‑149 (MQ=255)
                                                                                                                                        ccTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGcac         <  7:150023/149‑1 (MQ=255)
                                                                                                                                        ccTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGATGCAACGGCTGATCGACGACCAGCAGGca          >  3:139205/1‑148 (MQ=255)
                                                                                                                                         cTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGca          >  3:96292/1‑147 (MQ=255)
                                                                                                                                         cTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGca          >  1:69581/1‑147 (MQ=255)
                                                                                                                                               cGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGACGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGttt  <  5:397888/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
TGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCGTTT  >  NC_002947/5857822‑5858113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑