Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,970,527 C→T 6.2% R256H (CGC→CAC)  PP_5236 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,970,5270CT 6.2% 294.2 / 5.4 97R256H (CGC→CAC) PP_5236hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (47/44);  new base T (4/2);  total (51/46)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01

CGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGG  >  NC_002947/5970381‑5970669
                                                                                                                                                  |                                                                                                                                               
cGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCgg                                                                                                                                               <  3:99114/149‑1 (MQ=255)
cGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCgg                                                                                                                                               <  7:55903/149‑1 (MQ=255)
       cttcACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcagca                                                                                                                                        <  2:355321/146‑1 (MQ=255)
       cTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCTAGCGGGgcag                                                                                                                                          >  1:94860/1‑147 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCTGCCCCGCTGGGGcggcagc                                                                                                                                      <  7:315433/148‑2 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGTGGGGCAGCAgg                                                                                                                                      <  1:242697/148‑1 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGcagcag                                                                                                                                       <  6:290511/147‑1 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAgg                                                                                                                                      <  4:14206/148‑1 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAgg                                                                                                                                      >  8:240286/1‑148 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAgg                                                                                                                                      <  8:361338/148‑1 (MQ=255)
             cTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGTCGCCCCAGCGGGGCAGCAGGTaca                                                                                                                                  >  8:154961/1‑149 (MQ=255)
                  cAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGc                                                                                                                              >  1:46951/1‑148 (MQ=255)
                   aGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGc                                                                                                                              >  6:201556/1‑147 (MQ=255)
                     ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAgg                                                                                                                          >  8:367812/1‑149 (MQ=255)
                     ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAgg                                                                                                                          <  5:26725/149‑1 (MQ=255)
                     ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAgg                                                                                                                          >  6:314254/1‑149 (MQ=255)
                           gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTgg                                                                                                                    <  2:46951/149‑1 (MQ=255)
                            cAGCTTCAGCCGGCCTTCGCCAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCACGGGCCTGGa                                                                                                                   >  6:6080/1‑149 (MQ=255)
                             aGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGAt                                                                                                                  <  7:367812/149‑1 (MQ=255)
                              gCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATc                                                                                                                 <  2:341751/149‑1 (MQ=255)
                                ttCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGc                                                                                                               >  2:82123/1‑149 (MQ=255)
                                   aGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                            <  3:309397/149‑1 (MQ=255)
                                    gCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCg                                                                                                           <  6:252709/149‑1 (MQ=255)
                                     ccGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCg                                                                                                           >  4:44749/1‑148 (MQ=255)
                                      cGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGGCCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGaa                                                                                                         <  1:69957/149‑1 (MQ=255)
                                      cGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCg                                                                                                           >  1:38539/1‑147 (MQ=255)
                                       ggCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAag                                                                                                        <  4:321389/149‑1 (MQ=255)
                                        gCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagc                                                                                                       <  8:55820/149‑1 (MQ=255)
                                         ccTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagca                                                                                                      <  5:290058/149‑1 (MQ=255)
                                          cTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagcag                                                                                                     >  2:322312/1‑149 (MQ=255)
                                              gACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCggg                                                                                                 >  8:360242/1‑149 (MQ=255)
                                                cAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCgggg                                                                                               <  2:118154/149‑1 (MQ=255)
                                                 aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCTCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggc                                                                                              >  2:70065/1‑149 (MQ=255)
                                                 aGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggc                                                                                              >  4:380296/1‑149 (MQ=255)
                                                   ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                            >  4:68844/1‑133 (MQ=255)
                                                   ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                            <  3:68844/133‑1 (MQ=255)
                                                   ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcgg                                                                                            >  2:207537/1‑149 (MQ=255)
                                                   ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcgg                                                                                            <  8:334338/149‑1 (MQ=255)
                                                   ccATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcgg                                                                                            >  4:166740/1‑149 (MQ=255)
                                                    cATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcggg                                                                                           <  6:168693/149‑1 (MQ=255)
                                                    cATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcggg                                                                                           <  5:6080/149‑1 (MQ=255)
                                                     aTCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCtt                                                                                                             <  1:130042/130‑1 (MQ=255)
                                                     aTCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCtt                                                                                                             >  2:130042/1‑130 (MQ=255)
                                                     aTCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAACGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcgggc                                                                                          >  6:85210/1‑149 (MQ=255)
                                                          gcgcagcgcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCa                                                                                     <  1:82123/149‑1 (MQ=255)
                                                            gcagcgcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAgc                                                                                   >  7:298028/1‑149 (MQ=255)
                                                              agcgcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAgcgc                                                                                 <  3:380296/149‑1 (MQ=255)
                                                                   aTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGgc                                                                            <  5:7082/149‑1 (MQ=255)
                                                                       cGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCa                                                                         <  7:258673/148‑1 (MQ=255)
                                                                       cGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCa                                                                         <  5:314254/148‑1 (MQ=255)
                                                                        gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGCCCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGc                                                                       >  4:229254/1‑149 (MQ=255)
                                                                        gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGc                                                                       >  2:232127/1‑149 (MQ=255)
                                                                              gCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑aa                                                                >  8:267524/1‑149 (MQ=255)
                                                                              gCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑a                                                                 >  4:203659/1‑148 (MQ=255)
                                                                              gCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGG‑aa                                                                >  2:281038/1‑149 (MQ=255)
                                                                                tCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑aa                                                                >  2:358226/1‑147 (MQ=255)
                                                                                     ccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACCCAGCAAGGGCCTGGATCGCTTCGAAGCCGCCGGGGCGGGCGATCCGCGCATGGCGCAGGACGT‑AATAGCGAg                                                         >  8:333725/1‑149 (MQ=255)
                                                                                      cAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAgg                                                        <  5:201556/149‑1 (MQ=255)
                                                                                      cAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAgg                                                        <  8:298028/149‑1 (MQ=255)
                                                                                                acaGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGttt                                              <  3:203659/149‑1 (MQ=255)
                                                                                                  aGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGtttt                                             >  7:184920/1‑148 (MQ=255)
                                                                                                         cATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTc                                      >  2:387301/1‑148 (MQ=255)
                                                                                                         cATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTc                                      >  2:1705/1‑148 (MQ=255)
                                                                                                         cATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTc                                      >  1:177979/1‑148 (MQ=255)
                                                                                                         cATTGACCAACAGCTAGAACGCATCAAGGCTGACGCCCCAGCGGGGCAGCAGGTACACCGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGAATGGCGCAGCCAGT‑AATATCGAGGGTCCAGGTTTTCATggac                                      >  3:242658/1‑146 (MQ=255)
                                                                                                          aTTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTTGTTTTCATGGTCGa                                    >  1:190229/1‑149 (MQ=255)
                                                                                                                cAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCt                              >  3:215416/1‑149 (MQ=255)
                                                                                                                 aaCAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCtt                             <  4:215416/149‑1 (MQ=255)
                                                                                                                  aCAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTc                                                  >  8:66813/1‑127 (MQ=255)
                                                                                                                  aCAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTc                                                  <  7:66813/127‑1 (MQ=255)
                                                                                                                   cAGCTCGAACGCATCAAGGCTGCCGCCTCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTg                            <  1:70065/149‑1 (MQ=255)
                                                                                                                    aGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                          <  8:184920/149‑1 (MQ=255)
                                                                                                                    aGCTCGAACGCATCAAGGCTGCCGCCCCAACGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                          <  5:85210/149‑1 (MQ=255)
                                                                                                                      cTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCa                        <  1:358226/149‑1 (MQ=255)
                                                                                                                      cTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGGCCTCGGTTTCATGGTCGCCACGCTTGcc                          >  2:82841/1‑147 (MQ=255)
                                                                                                                       tCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                          >  6:68342/1‑146 (MQ=255)
                                                                                                                        cGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                          >  6:243710/1‑145 (MQ=255)
                                                                                                                        cGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                          >  8:318652/1‑145 (MQ=255)
                                                                                                                               tgtgtacctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGcgcg               >  1:174032/8‑149 (MQ=255)
                                                                                                                                tccatgCTGCCGGCCCAGCGGTGCAGCAGGTACACAGCAAGGGCCTGGATGGCTTCTCAGCAGCCGGGTCGGGCGATCAGCGCATGGCTCAGCCAGT‑AATAGCGAGGGGCCTCGTTTTCATGGTCGACCCTCTTGCCCAGATAGCGCgg              <  4:242658/144‑1 (MQ=255)
                                                                                                                                tCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg              <  1:82841/149‑1 (MQ=255)
                                                                                                                                tCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg              >  2:277864/1‑149 (MQ=255)
                                                                                                                                 cAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg              >  7:54773/1‑148 (MQ=255)
                                                                                                                                  aaGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg              >  8:315807/1‑147 (MQ=255)
                                                                                                                                    ggCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGc          >  6:389083/1‑149 (MQ=255)
                                                                                                                                    ggCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGc          <  7:318652/149‑1 (MQ=255)
                                                                                                                                     cctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATgg                                        >  4:308883/2‑118 (MQ=255)
                                                                                                                                      ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa        >  4:27460/1‑149 (MQ=255)
                                                                                                                                      ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa        >  2:211583/1‑149 (MQ=255)
                                                                                                                                      cTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa        <  7:315807/149‑1 (MQ=255)
                                                                                                                                       tGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGAc       >  2:23054/1‑149 (MQ=255)
                                                                                                                                       tGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTCTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGAc       >  7:207595/1‑149 (MQ=255)
                                                                                                                                         ccgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGAc       <  2:38539/147‑1 (MQ=255)
                                                                                                                                         ccgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACt      <  2:177979/148‑1 (MQ=255)
                                                                                                                                           gccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACtgtg   >  3:105360/1‑149 (MQ=255)
                                                                                                                                            ccccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTgg  <  7:154961/149‑1 (MQ=255)
                                                                                                                                            ccccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTgg  <  5:68342/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGT‑AATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGG  >  NC_002947/5970381‑5970669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑