BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,984,832	G→A	7.0%	D96D (GAC→GAT)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,984,832	0	G	A	7.0%	312.1 / 8.2	102	D96D (GAC→GAT)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base G (51/43); new base A (2/5); total (53/49)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGC  >  NC_002947/5984685‑5984978

gTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGt                                                                                                                                                        <  6:238433/149‑1 (MQ=255)

gTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGt                                                                                                                                                        >  6:366760/1‑149 (MQ=255)

 ttGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTc                                                                                                                                                       <  6:388422/149‑1 (MQ=255)

   gAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCTGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCtt                                                                                                                                                     >  2:339172/1‑149 (MQ=255)

     aGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCg                                                                                                                                                   >  1:109564/1‑149 (MQ=255)

     aGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCg                                                                                                                                                   >  7:94940/1‑149 (MQ=255)

      ggTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGt                                                                                                                                                  >  8:263503/1‑149 (MQ=255)

       gTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGtt                                                                                                                                                 >  1:152754/1‑149 (MQ=255)

       gTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGtt                                                                                                                                                 >  7:4218/1‑149 (MQ=255)

       gTGACCGATGCCTCGGGCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGtt                                                                                                                                                 >  7:27213/1‑149 (MQ=255)

           ccGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTcc                                                                                                                                             <  7:128521/149‑1 (MQ=255)

                  aTCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGt                                                                                                                                      >  3:123113/1‑149 (MQ=255)

                  aTCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTAACGAAGTCTTCGTTGTCCCAGGCGt                                                                                                                                      >  3:373132/1‑149 (MQ=255)

                      gTCACCACGAACACTTCAAAACCTTCCGCCCGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGAt                                                                                                                                  <  8:344454/149‑1 (MQ=255)

                         accacGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCTGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTg                                                                                                                               <  1:339172/149‑1 (MQ=255)

                          ccacGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTgg                                                                                                                              <  5:64055/149‑1 (MQ=255)

                          ccacGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTgg                                                                                                                              <  5:141962/149‑1 (MQ=255)

                          ccacGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTgg                                                                                                                              >  8:161544/1‑149 (MQ=255)

                           cacGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACTCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGc                                                                                                                             >  7:81594/1‑149 (MQ=255)

                           cacGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGc                                                                                                                             >  7:272183/1‑149 (MQ=255)

                             cGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCg                                                                                                                           <  8:4218/149‑1 (MQ=255)

                             cGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCg                                                                                                                           <  6:75096/149‑1 (MQ=255)

                                    ttCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACggg                                                                                                                    >  8:38458/1‑149 (MQ=255)

                                    ttCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTAGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACggg                                                                                                                    >  7:9352/1‑149 (MQ=255)

                                     tCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGtt                                                                                                                                     >  8:4238/1‑131 (MQ=255)

                                     tCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGtt                                                                                                                                     <  7:4238/131‑1 (MQ=255)

                                        aagccTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAAt                                                                                                                >  7:206223/4‑149 (MQ=255)

                                        aaaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACgg                                                                                                                     <  5:380333/144‑1 (MQ=255)

                                        aaaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACgg                                                                                                                     >  6:380333/1‑144 (MQ=255)

                                        aaaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAAt                                                                                                                >  2:355845/1‑149 (MQ=255)

                                            cTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTAc                                                                                                            <  7:263503/149‑1 (MQ=255)

                                            cTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTAc                                                                                                            <  7:126159/149‑1 (MQ=255)

                                               ccGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACgg                                                                                                          <  7:336195/148‑1 (MQ=255)

                                                   cAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCAt                                                                                                     <  1:355845/149‑1 (MQ=255)

                                                    aGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATc                                                                                                    >  6:251158/1‑149 (MQ=255)

                                                      cgcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCg                                                                                                  <  4:123113/149‑1 (MQ=255)

                                                       gcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCgg                                                                                                 <  8:206223/149‑1 (MQ=255)

                                                          gACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGaa                                                                                              >  1:311527/1‑149 (MQ=255)

                                                           aCAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAAc                                                                                             <  3:224220/149‑1 (MQ=255)

                                                              gggTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGc                                                                                                                             <  3:328336/114‑1 (MQ=255)

                                                              gggTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGc                                                                                                                             >  4:328336/1‑114 (MQ=255)

                                                                   gggAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCttt                                                                                     <  8:272183/149‑1 (MQ=255)

                                                                     gAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTCGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCa                                                                                   <  8:9352/149‑1 (MQ=255)

                                                                      aaGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAg                                                                                  >  1:39401/1‑149 (MQ=255)

                                                                         gCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTc                                                                               >  5:125406/1‑149 (MQ=255)

                                                                         gCCACGCACACATCGGTCACCACACCGGCGAAGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTc                                                                               >  1:42708/1‑149 (MQ=255)

                                                                          ccACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCg                                                                              <  5:251158/149‑1 (MQ=255)

                                                                           cACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCgg                                                                             >  1:258316/1‑149 (MQ=255)

                                                                            aCGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCATGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                            <  5:31321/149‑1 (MQ=255)

                                                                               cacacaTCGGTCACCACTCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CAc                                                                      <  8:81594/149‑1 (MQ=255)

                                                                                 cacaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTCACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCa                                                                    <  4:373132/149‑1 (MQ=255)

                                                                                  acaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCa                                                                                   <  6:333138/136‑1 (MQ=255)

                                                                                  acaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATCTACGGCGCATCCGGGAACATCTCTTTCa                                                                                   >  5:333138/1‑136 (MQ=255)

                                                                                      cGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCgg‑g                                                             <  2:389177/149‑1 (MQ=255)

                                                                                      cGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCgg‑g                                                             >  6:305306/1‑149 (MQ=255)

                                                                                        gTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTc                                                                                    >  5:364263/1‑129 (MQ=255)

                                                                                        gTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTc                                                                                    <  6:364263/129‑1 (MQ=255)

                                                                                           accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCAtt                                                        >  3:381649/1‑149 (MQ=255)

                                                                                             cacaCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCg                                                      >  3:52780/1‑149 (MQ=255)

                                                                                              acacCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCgg                                                     <  2:42708/149‑1 (MQ=255)

                                                                                                  cGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCt                                                 >  3:205370/1‑149 (MQ=255)

                                                                                                  cGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCt                                                 >  5:140999/1‑149 (MQ=255)

                                                                                                  cGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCt                                                 >  4:35237/1‑149 (MQ=255)

                                                                                                     cGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGc                                              <  7:161544/149‑1 (MQ=255)

                                                                                                     cGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGc                                              <  8:27213/149‑1 (MQ=255)

                                                                                                      gatgatCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCt                                             >  6:260696/1‑149 (MQ=255)

                                                                                                           tCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAg                                        >  4:266994/1‑149 (MQ=255)

                                                                                                           tCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAg                                        >  4:261754/1‑149 (MQ=255)

                                                                                                             aGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCt                                      >  6:167555/1‑149 (MQ=255)

                                                                                                              gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                     >  4:107213/1‑149 (MQ=255)

                                                                                                              gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                     >  3:10022/1‑149 (MQ=255)

                                                                                                              gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                     >  2:368668/1‑149 (MQ=255)

                                                                                                                  cttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtgg                                 <  5:305306/149‑1 (MQ=255)

                                                                                                                   ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTggaggt                                >  7:208326/1‑149 (MQ=255)

                                                                                                                      cGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                              <  4:205370/148‑1 (MQ=255)

                                                                                                                      cGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                              <  2:2631/148‑1 (MQ=255)

                                                                                                                       ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAgg                            <  4:381649/149‑1 (MQ=255)

                                                                                                                       ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                             >  1:334892/1‑148 (MQ=255)

                                                                                                                        gCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                           <  1:368668/149‑1 (MQ=255)

                                                                                                                         cccGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                          <  5:260696/149‑1 (MQ=255)

                                                                                                                           cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                        >  7:315786/1‑149 (MQ=255)

                                                                                                                                   tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                    >  1:79639/6‑129 (MQ=11)

                                                                                                                                   tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                    <  2:79639/124‑1 (MQ=11)

                                                                                                                                    gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                 <  2:39401/147‑1 (MQ=255)

                                                                                                                                    gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                 <  7:38458/147‑1 (MQ=255)

                                                                                                                                     accgCCTAGACGAAATCCTCCTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc              <  8:261008/146‑1 (MQ=11)

                                                                                                                                     aTTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCACGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                      <  1:81514/142‑1 (MQ=255)

                                                                                                                                     aTTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                      >  2:81514/1‑141 (MQ=255)

                                                                                                                                         ccTTGACGAAATCCTCGTTGTCCCCGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg          <  6:214536/149‑1 (MQ=14)

                                                                                                                                          cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg          >  3:270087/1‑148 (MQ=255)

                                                                                                                                             gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGc                                                   <  3:53795/104‑1 (MQ=14)

                                                                                                                                             gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGc                                                   >  4:53795/1‑104 (MQ=14)

                                                                                                                                              aCGAACGCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt     <  2:152754/149‑1 (MQ=255)

                                                                                                                                               cGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCGTGc                                              >  4:330258/1‑107 (MQ=255)

                                                                                                                                               cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg    <  2:52431/149‑1 (MQ=255)

                                                                                                                                                gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTGGTGTTAGAGGA‑CCGGAGGCAATAGGGCCTTGATCGAAGCTGGTGGTGAGGATGTTCGGCAGGCCAAAGAAc       >  1:324330/1‑149 (MQ=18)

                                                                                                                                                gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                       <  6:100416/129‑1 (MQ=255)

                                                                                                                                                gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                       >  5:100416/1‑129 (MQ=255)

                                                                                                                                                 aaGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc  <  6:125406/149‑1 (MQ=255)

                                                                                                                                                  aGTCTTCGTTGTCACAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc  >  5:129721/1‑148 (MQ=255)

GTTGAAGGTGCCCGAGGCATCGGTCACCACGAACACTTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGC  >  NC_002947/5984685‑5984978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`