BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,984,865	A→G	22.3%	R85R (CGT→CGC)	ycaC‑II ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,984,865	0	A	G	22.3%	193.5 / 53.3	103	R85R (CGT→CGC)	ycaC‑II	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (40/40); new base G (11/12); total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 104 total aligned reads displayed.

TTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATT  >  NC_002947/5984721‑5985013

ttCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACggg                                                                                                                                                       >  8:38458/1‑149 (MQ=255)

ttCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTAGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACggg                                                                                                                                                       >  7:9352/1‑149 (MQ=255)

    aagccTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAAt                                                                                                                                                   >  7:206223/4‑149 (MQ=255)

    aaaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACgg                                                                                                                                                        <  5:380333/144‑1 (MQ=255)

    aaaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACgg                                                                                                                                                        >  6:380333/1‑144 (MQ=255)

    aaaCCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAAt                                                                                                                                                   >  2:355845/1‑149 (MQ=255)

        cTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTAc                                                                                                                                               <  7:126159/149‑1 (MQ=255)

        cTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTAc                                                                                                                                               <  7:263503/149‑1 (MQ=255)

           ccGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACgg                                                                                                                                             <  7:336195/148‑1 (MQ=255)

               cAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCAt                                                                                                                                        <  1:355845/149‑1 (MQ=255)

                aGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATc                                                                                                                                       >  6:251158/1‑149 (MQ=255)

                  cgcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCg                                                                                                                                     <  4:123113/149‑1 (MQ=255)

                   gcCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCgg                                                                                                                                    <  8:206223/149‑1 (MQ=255)

                      gACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGaa                                                                                                                                 >  1:311527/1‑149 (MQ=255)

                       aCAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAAc                                                                                                                                <  3:224220/149‑1 (MQ=255)

                               gggAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCttt                                                                                                                        <  8:272183/149‑1 (MQ=255)

                                 gAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTCGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCa                                                                                                                      <  8:9352/149‑1 (MQ=255)

                                  aaGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAg                                                                                                                     >  1:39401/1‑149 (MQ=255)

                                     gCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTc                                                                                                                  >  5:125406/1‑149 (MQ=255)

                                     gCCACGCACACATCGGTCACCACACCGGCGAAGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTc                                                                                                                  >  1:42708/1‑149 (MQ=255)

                                      ccACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCg                                                                                                                 <  5:251158/149‑1 (MQ=255)

                                       cACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCgg                                                                                                                >  1:258316/1‑149 (MQ=255)

                                        aCGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCATGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCggg                                                                                                               <  5:31321/149‑1 (MQ=255)

                                           cacacaTCGGTCACCACTCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CAc                                                                                                         <  8:81594/149‑1 (MQ=255)

                                             cacaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTCACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCa                                                                                                       <  4:373132/149‑1 (MQ=255)

                                              acaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCa                                                                                                                      <  6:333138/136‑1 (MQ=255)

                                              acaTCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATCTACGGCGCATCCGGGAACATCTCTTTCa                                                                                                                      >  5:333138/1‑136 (MQ=255)

                                                  cGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCgg‑g                                                                                                <  2:389177/149‑1 (MQ=255)

                                                  cGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCgg‑g                                                                                                >  6:305306/1‑149 (MQ=255)

                                                    gTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTc                                                                                                                       >  5:364263/1‑129 (MQ=255)

                                                    gTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTc                                                                                                                       <  6:364263/129‑1 (MQ=255)

                                                       accacACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCAtt                                                                                           >  3:381649/1‑149 (MQ=255)

                                                         cacaCCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCg                                                                                         >  3:52780/1‑149 (MQ=255)

                                                          acacCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCgg                                                                                        <  2:42708/149‑1 (MQ=255)

                                                              cGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCt                                                                                    >  5:140999/1‑149 (MQ=255)

                                                              cGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCt                                                                                    >  4:35237/1‑149 (MQ=255)

                                                              cGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCt                                                                                    >  3:205370/1‑149 (MQ=255)

                                                                 cGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGc                                                                                 <  7:161544/149‑1 (MQ=255)

                                                                 cGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGc                                                                                 <  8:27213/149‑1 (MQ=255)

                                                                      atCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCg                                                                              >  5:182692/1‑147 (MQ=255)

                                                                       tCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                           >  4:266994/1‑149 (MQ=255)

                                                                       tCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAg                                                                           >  4:261754/1‑149 (MQ=255)

                                                                         aGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCt                                                                         >  6:167555/1‑149 (MQ=255)

                                                                          gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                        >  4:107213/1‑149 (MQ=255)

                                                                          gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                        >  3:10022/1‑149 (MQ=255)

                                                                          gTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtg                                                                        >  2:368668/1‑149 (MQ=255)

                                                                              cttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtggtgg                                                                    <  5:305306/149‑1 (MQ=255)

                                                                               ttgcGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTggaggt                                                                   >  7:208326/1‑149 (MQ=255)

                                                                                  cGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                 <  4:205370/148‑1 (MQ=255)

                                                                                  cGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGa                                                                 <  2:2631/148‑1 (MQ=255)

                                                                                   ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAgg                                                               <  4:381649/149‑1 (MQ=255)

                                                                                   ggCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAg                                                                >  1:334892/1‑148 (MQ=255)

                                                                                    gCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGa                                                              <  1:368668/149‑1 (MQ=255)

                                                                                     cccGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGAt                                                             <  5:260696/149‑1 (MQ=255)

                                                                                       cGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATgg                                                           >  7:315786/1‑149 (MQ=255)

                                                                                               tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                                                       >  1:79639/6‑129 (MQ=11)

                                                                                               tcaccgCCTTGACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCtgg                                                                       <  2:79639/124‑1 (MQ=11)

                                                                                                gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                    <  2:39401/147‑1 (MQ=255)

                                                                                                gATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAg                                                    <  7:38458/147‑1 (MQ=255)

                                                                                                 accgCCTAGACGAAATCCTCCTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGcc                                                 <  8:261008/146‑1 (MQ=11)

                                                                                                 aTTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCACGCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTc                                                         <  1:81514/142‑1 (MQ=255)

                                                                                                     ccTTGACGAAATCCTCGTTGTCCCCGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg                                             <  6:214536/149‑1 (MQ=14)

                                                                                                      cTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAg                                             >  3:270087/1‑148 (MQ=255)

                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGc                                                                                      <  3:53795/104‑1 (MQ=14)

                                                                                                         gACGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGc                                                                                      >  4:53795/1‑104 (MQ=14)

                                                                                                          aCGAACGCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACtt                                        <  2:152754/149‑1 (MQ=255)

                                                                                                           cGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCGTGc                                                                                 >  4:330258/1‑107 (MQ=255)

                                                                                                           cGAAATCCTCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTg                                       <  2:52431/149‑1 (MQ=255)

                                                                                                            gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTGGTGTTAGAGGA‑CCGGAGGCAATAGGGCCTTGATCGAAGCTGGTGGTGAGGATGTTCGGCAGGCCAAAGAAc                                          >  1:324330/1‑149 (MQ=18)

                                                                                                            gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                          >  5:100416/1‑129 (MQ=255)

                                                                                                            gAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGt                                                          <  6:100416/129‑1 (MQ=255)

                                                                                                             aaGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                     <  6:125406/149‑1 (MQ=255)

                                                                                                              aGTCTTCGTTGTCACAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGc                                     >  5:129721/1‑148 (MQ=255)

                                                                                                               atcctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCa                                   <  7:69621/145‑1 (MQ=255)

                                                                                                                 cctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                                   >  3:180980/3‑131 (MQ=255)

                                                                                                                 cctCGTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAgg                                                   <  4:180980/129‑1 (MQ=255)

                                                                                                                     gTTGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTc                               <  5:324430/147‑1 (MQ=255)

                                                                                                                      ttGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGcc                            >  1:149483/1‑149 (MQ=255)

                                                                                                                      ttGTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGcc                            >  3:20122/1‑149 (MQ=255)

                                                                                                                        gTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGccc                           <  2:109564/148‑1 (MQ=255)

                                                                                                                        gTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGccc                           >  1:7861/1‑148 (MQ=255)

                                                                                                                        gTCCCAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGccc                           >  5:330133/1‑148 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                       >  6:291486/1‑149 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                       <  2:311527/149‑1 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                       >  3:48654/1‑149 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                       >  4:192123/1‑149 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAgg                       >  7:194435/1‑149 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                        <  4:152974/148‑1 (MQ=255)

                                                                                                                           ccAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAg                        <  2:351151/148‑1 (MQ=255)

                                                                                                                            cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                      >  3:75535/1‑149 (MQ=255)

                                                                                                                            cAGGCGTTGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGc                      >  6:372938/1‑149 (MQ=255)

                                                                                                                             aGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGcc                     <  6:140999/149‑1 (MQ=255)

                                                                                                                                cGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGc                  <  6:182692/149‑1 (MQ=255)

                                                                                                                                  ttGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCAc                <  5:366760/149‑1 (MQ=255)

                                                                                                                                  ttGATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCAc                <  5:372920/149‑1 (MQ=255)

                                                                                                                                    gATCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCATCACgt              >  5:211444/1‑149 (MQ=255)

                                                                                                                                     aTCTGGCCAGGGCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgt              <  7:320704/148‑1 (MQ=255)

                                                                                                                                          gCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACgttg            <  6:129721/145‑1 (MQ=255)

                                                                                                                                             gggACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCTGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGTACGTTGTTCTTGa     <  1:351950/149‑1 (MQ=255)

                                                                                                                                                aCGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAAtt  <  5:167555/149‑1 (MQ=255)

TTCAAAACCTTCCGCCAGCGCCGACAGGGTCGGGAAGGCCACGCACACATCGGTCACCACACCGGCGATGATCAGTTGCTTGCGGCCCGTGGCCTTGATTGCCTTGACGAAGTCTTCGTTGTCCCAGGCGTTGATCTGGCCGGGACGGGCAATGTACGGCGCATCCGGGAACATCTCTTTCAGCTCGGG‑‑‑CACCA‑GCGG‑GCCATTCGGGCCTTGCTCGAAGCTGGTGGTGAGGATGGTCGGCAGGCCAAAGAACTTGGCCAGGTCGCCCAAGGCCAGCACGTTGTTCTTGAATT  >  NC_002947/5984721‑5985013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`